EC 18.104.22.168 - Fruit bromelain
IntEnz Enzyme Nomenclature
fruit bromelain FA2
- Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg┼NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-NHMec (c.f. stem bromelain).
From the pineapple plant, Ananas comosus. Scarcely inhibited by chicken cystatin. Another cysteine endopeptidase, with similar action on small molecule substrates, pinguinain (formerly EC 22.214.171.124), is obtained from the related plant, Bromelia pinguin, but pinguinain differs from fruit bromelain in being inhibited by chicken cystatin . In peptidase family C1 (papain family). Formerly EC 126.96.36.199 and included in EC 188.8.131.52.
Links to other databases
Antigenic determinant common to four kinds of thiol proteases of plant origin.J. Biochem. (Tokyo) 74: 635-637 (1973). [PMID: 4127920]
Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2.J. Biochem. (Tokyo) 79: 1223-1234 (1976). [PMID: 956152]
Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains.J. Biochem. (Tokyo) 98: 219-228 (1985). [PMID: 4044551]
The cysteine proteinases of the pineapple plant.Biochem. J. 266: 869-875 (1990). [PMID: 2327970]
[EC 184.108.40.206 created 1965 as EC 220.127.116.11, transferred 1972 to EC 18.104.22.168, part transferred 1992 to EC 22.214.171.124]