EC 220.127.116.11 - Stem bromelain
IntEnz Enzyme Nomenclature
- Broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg┼NHMec amongst small molecule substrates.
The most abundant of the cysteine endopeptidases of the stem of the pineapple plant, Ananas comosus. Distinct from the bromelain found in the pineapple fruit (EC 18.104.22.168). Scarcely inhibited by chicken cystatin and also very slowly inactivated by E-64. In peptidase family C1 (papain family). Formerly EC 22.214.171.124 and included in 126.96.36.199.
Links to other databases
Cysteine proteinases.In: Neuberger, A. and Brocklehurst, K. (Eds.)
New Comprehensive Biochemistryvol. 16, Elsevier, Amsterdam, 1987, 39-158
Ananain: a novel cysteine proteinase found in pineapple stem.Arch. Biochem. Biophys. 267: 262-270 (1988). [PMID: 3196029]
Stem bromelain: amino acid sequence and implications for weak binding to cystatin.FEBS Lett. 247: 419-429 (1989). [PMID: 2714443]
The cysteine proteinases of the pineapple plant.Biochem. J. 266: 869-875 (1990). [PMID: 2327970]
[EC 188.8.131.52 created 1965 as EC 184.108.40.206, transferred 1972 to EC 220.127.116.11, part transferred 1992 to EC 18.104.22.168]