EC 220.127.116.11 - C-terminal processing peptidase
IntEnz Enzyme Nomenclature
photosystem II D1 protein processing peptidase
- The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala┼Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala. In the plant chloroplast, the enzyme removes the C-terminal extension of the D1 polypeptide of photosystem II.
Proteolytic processing of the D1 protein of photosystem II is necessary to allow the light-driven assembly of the tetranuclear manganese cluster, which is responsible for photosynthetic water oxidation. The recognition of the substrate is mediated by a PDZ domain, a small protein module that promotes protein-protein interactions by binding to internal or C-terminal sequences of their partner proteins. Type example of peptidase family S41.
Links to other databases
Tsp protease.In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.) Handbook of Proteolytic Enzymes, Academic Press, London, 1998, 460-461
Substrate recognition through a PDZ domain in tail-specific protease.Biochemistry 39: 3149-3155 (2000). [PMID: 10715137]
Crystal structures of the photosystem II D1 C-terminal processing protease.Nat. Struct. Biol. 7: 749-753 (2000). [PMID: 10966643]
[EC 18.104.22.168 created 2001]