EC 3.2.1.81 - β-agarase

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IntEnz Enzyme Nomenclature
EC 3.2.1.81

Names

Accepted name:
β-agarase
Other names:
agarase [ambiguous]
AgaA
AgaB
endo-β-agarase
agarose 3-glycanohydrolase [incorrect]
Systematic name:
agarose 4-glycanohydrolase

Reaction

Comments:

Also acts on porphyran, but more slowly [1]. This enzyme cleaves the β-(1→4) linkages of agarose in a random manner with retention of the anomeric-bond configuration, producing β-anomers that give rise progressively to α-anomers when mutarotation takes place [6]. The end products of hydrolysis are neoagarotetraose and neoagarohexaose in the case of AgaA from the marine bacterium Zobellia galactanivorans, and neoagarotetraose and neoagarobiose in the case of AgaB [6].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033916
CAS Registry Number: 37288-57-6
UniProtKB/Swiss-Prot: (11) [show] [UniProt]

References

  1. Duckworth, M. and Turvey, J.R.
    The action of a bacterial agarase on agarose, porphyran and alkali-treated porphyran.
    Biochem. J. 113: 687-692 (1969). [PMID: 5386190]
  2. Allouch, J., Jam, M., Helbert, W., Barbeyron, T., Kloareg, B., Henrissat, B. and Czjzek, M.
    The three-dimensional structures of two β-agarases.
    J. Biol. Chem. 278: 47171-47180 (2003). [PMID: 12970344]
  3. Ohta, Y., Nogi, Y., Miyazaki, M., Li, Z., Hatada, Y., Ito, S. and Horikoshi, K.
    Enzymatic properties and nucleotide and amino acid sequences of a thermostable β-agarase from the novel marine isolate, JAMB-A94.
    Biosci. Biotechnol. Biochem. 68: 1073-1081 (2004). [PMID: 15170112]
  4. Ohta, Y., Hatada, Y., Nogi, Y., Miyazaki, M., Li, Z., Akita, M., Hidaka, Y., Goda, S., Ito, S. and Horikoshi, K.
    Enzymatic properties and nucleotide and amino acid sequences of a thermostable β-agarase from a novel species of deep-sea Microbulbifer.
    Appl. Microbiol. Biotechnol. 64: 505-514 (2004). [PMID: 15088129]
  5. Sugano, Y., Terada, I., Arita, M., Noma, M., Matsumoto, T.
    Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107.
    Appl. Environ. Microbiol. 59: 1549-1554 (1993). [PMID: 8517750]
  6. Jam, M., Flament, D., Allouch, J., Potin, P., Thion, L., Kloareg, B., Czjzek, M., Helbert, W., Michel, G. and Barbeyron, T.
    The endo-β-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours.
    Biochem. J. 385: 703-713 (2005). [PMID: 15456406]

[EC 3.2.1.81 created 1972, modified 2006]