EC 126.96.36.199 - Chitosanase
IntEnz Enzyme Nomenclature
- Endohydrolysis of β-(1→4)-linkages between D-glucosamine residues in a partly acetylated chitosan
A whole spectrum of chitosanases are now known (for more details, see http://pages.usherbrooke.ca/rbrzezinski/). They can hydrolyse various types of links in chitosan. The only constant property is the endohydrolysis of GlcN-GlcN links, which is common to all known chitosanases. One known chitosanase is limited to this link recognition , while the majority can also recognize GlcN-GlcNAc links or GlcNAc-GlcN links but not both. They also do not recognize GlcNAc-GlcNAc links in partly acetylated chitosan.
Links to other databases
Purification and mode of action of a chitosanase from Penicillium islandicum.J. Gen. Microbiol. 126: 151-165 (1981).
Crystal structure of chitosanase from Bacillus circulans MH-K1 at 1.6-Å resolution and its substrate recognition mechanism.J. Biol. Chem. 274: 30818-30825 (1999). [PMID: 10521473]
Action pattern of Bacillus sp. No. 7-M chitosanase on partially N-acetylated chitosan.Biosci. Biotechnol. Biochem. 56: 448-453 (1992). [PMID: 1368330]
X-ray structure of an anti-fungal chitosanase from Streptomyces N174.Nat. Struct. Biol. 3: 155-162 (1996). [PMID: 8564542]
[EC 188.8.131.52 created 1990, modified 2004]