EC - Crossover junction endodeoxyribonuclease

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
crossover junction endodeoxyribonuclease
Other names:
Hje endonuclease
Holliday junction endonuclease CCE1
Holliday junction resolvase
Holliday junction-cleaving endonuclease
Holliday junction-resolving endoribonuclease
RusA Holliday junction resolvase
RusA endonuclease
RuvC endonuclease
SpCCe1 Holliday junction resolvase
crossover junction endoribonuclease
cruciform-cutting endonuclease
endo X3
endonuclease RuvC
endonuclease VII
endonuclease X3
resolving enzyme CCE1
Systematic name:



The enzyme from Saccharomyces cerevisiae has no endonuclease or exonuclease activity on single-stranded or double-stranded DNA molecules that do not contain Holliday junctions.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC01025
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008821
CAS Registry Number: 99676-43-4
UniProtKB/Swiss-Prot: (571) [show] [UniProt]


  1. Symington, L.S. and Kolodner, R.
    Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions.
    Proc. Natl. Acad. Sci. USA 82: 7247-7251 (1985). [PMID: 9308179]
  2. Shida, T., Iwasaki, H., Saito, A., Kyogoku, Y. and Shinagawa, H.
    Analysis of substrate specificity of the RuvC holliday junction resolvase with synthetic Holliday junctions.
    J. Biol. Chem. 271: 26105-26109 (1996). [PMID: 8824253]
  3. Shah, R., Cosstick, R. and West, S.C.
    The RuvC protein dimer resolves Holliday junctions by a dual incision mechanism that involves base-specific contacts.
    EMBO J. 16: 1464-1472 (1997). [PMID: 9135161]
  4. Fogg, J.M., Schofield, M.J., White, M.F. and Lilley, D.M.
    Sequence and functional-group specificity for cleavage of DNA junctions by RuvC of Escherichia coli.
    Biochemistry 38: 11349-11358 (1999). [PMID: 10471285]
  5. Middleton, C.L., Parker, J.L., Richard, D.J., White, M.F. and Bond, C.S.
    Crystallization and preliminary X-ray diffraction studies of Hje, a Holliday junction resolving enzyme from Sulfolobus solfataricus.
    Acta Crystallogr. D Biol. Crystallogr. 59: 171-173 (2003). [PMID: 12499561]

[EC created 1989, modified 2003]