EC 220.127.116.11 - Type I site-specific deoxyribonuclease
IntEnz Enzyme Nomenclature
adenosine triphosphate-dependent deoxyribonuclease
deoxyribonuclease (ATP- and S-adenosyl-L-methionine-dependent)
deoxyribonuclease (adenosine triphosphate-hydrolyzing)
type 1 site-specific deoxyribonuclease
type I restriction enzyme
- Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed.
This is a large group of enzymes which, together with those now listed as EC 18.104.22.168 (type II site-specific deoxyribonuclease) and EC 22.214.171.124 (type III site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC 3.1.23 and EC 3.1.24. They have an absolute requirement for ATP (or dATP) and S-adenosyl-L-methionine. They recognize specific short DNA sequences and cleave at sites remote from the recognition sequence. They are multifunctional proteins that also catalyse the reactions of EC 126.96.36.199 [site-specific DNA-methyltransferase (adenine-specific)] and EC 188.8.131.52 [site-specific DNA-methyltransferase (cytosine-specific)], with similar site specificity. A complete listing of all of these enzymes has been produced by R.J. Roberts and is available at http://rebase.neb.com/rebase/.
Links to other databases
Restriction enzymes and their isoschizomers.Nucleic Acids Res. 18: 2331-2365 (1990). [PMID: 2159140]
REBASE—restriction enzymes and methylases.Nucleic Acids Res. 29: 268-269 (2001). [PMID: 11125108]
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.Nucleic Acids Res. 31: 1805-1812 (2003). [PMID: 12654995]
[EC 184.108.40.206 created 1984 from EC 3.1.23 and EC 3.1.24]