EC - Holo-[acyl-carrier-protein] synthase

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IntEnz Enzyme Nomenclature


Accepted name:
holo-[acyl-carrier-protein] synthase
Other names:
acyl carrier protein holoprotein (holo-ACP) synthetase
acyl carrier protein synthetase
coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine transferase
holo-ACP synthase
holo-ACP synthetase
acyl carrier protein synthase
P-pant transferase
CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
4'-phosphopantetheinyl transferase
αaminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase
Systematic name:
CoA-[4'-phosphopantetheine]:apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase




Requires Mg2+. All polyketide synthases, fatty-acid synthases and non-ribosomal peptide synthases require post-translational modification of their constituent acyl-carrier-protein (ACP) domains to become catalytically active. The inactive apo-proteins are converted into their active holo-forms by transfer of the 4'-phosphopantetheinyl moiety of CoA to the sidechain hydroxy group of a conserved serine residue in each ACP domain [3]. The enzyme from human can activate both the ACP domain of the human cytosolic multifunctional fatty acid synthase and that associated with human mitochondria as well as peptidyl-carrier and acyl-carrier-proteins from prokaryotes [6]. Removal of the 4-phosphopantetheinyl moiety from holo-ACP is carried out by EC, [acyl-carrier-protein] phosphodiesterase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008897
CAS Registry Number: 37278-30-1
UniProtKB/Swiss-Prot: (581) [show] [UniProt]


  1. Elovson, J. and Vagelos, P.R.
    Acyl carrier protein. X. Acyl carrier protein synthetase.
    J. Biol. Chem. 243: 3603-3611 (1968). [PMID: 4872726]
  2. Prescott, D.J. and Vagelos, P.R.
    Acyl carrier protein.
    Adv. Enzymol. Relat. Areas Mol. Biol. 36: 269-311 (1972). [PMID: 4561013]
  3. Lambalot, R.H., Gehring, A.M., Flugel, R.S., Zuber, P., LaCelle, M., Marahiel, M.A., Reid, R., Khosla, C. and Walsh, C.T.
    A new enzyme superfamily - the phosphopantetheinyl transferases.
    Chem. Biol. 3: 923-936 (1996). [PMID: 8939709]
  4. Walsh, C.T., Gehring, A.M., Weinreb, P.H., Quadri, L.E.N. and Flugel, R.S.
    Post-translational modification of polyketide and nonribosomal peptide synthases.
    Curr. Opin. Chem. Biol. 1: 309-315 (1997). [PMID: 9667867]
  5. Mootz, H.D., Finking, R. and Marahiel, M.A.
    4'-phosphopantetheine transfer in primary and secondary metabolism of Bacillus subtilis.
    J. Biol. Chem. 276: 37289-37298 (2001). [PMID: 11489886]
  6. Joshi, A.K., Zhang, L., Rangan, V.S. and Smith, S.
    Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity.
    J. Biol. Chem. 278: 33142-33149 (2003). [PMID: 12815048]

[EC created 1972, modified 2006]