EC 2.5.1.60 - Protein geranylgeranyltransferase type II

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IntEnz Enzyme Nomenclature
EC 2.5.1.60

Names

Accepted name:
protein geranylgeranyltransferase type II
Other names:
GGTaseII
Rab geranylgeranyltransferase
RabGGTase
type II protein geranyl-geranyltransferase
Systematic name:
geranylgeranyl-diphosphate:protein-cysteine geranyltransferase

Reaction

Comments:

This enzyme, along with protein farnesyltransferase (EC 2.5.1.58) and protein geranylgeranyltransferase type I (EC 2.5.1.59), constitutes the protein prenyltransferase family of enzymes. Attaches geranylgeranyl groups to two C-terminal cysteines in Ras-related GTPases of a single family, the Rab family (Ypt/Sec4 in lower eukaryotes) that terminate in XXCC, XCXC and CCXX motifs. Reaction is entirely dependent on the Rab substrate being bound to Rab escort protein (REP). Post-translational modification with the geranylgeranyl moiety is essential for Rab GTPases to be able to control the processes of membrane docking and fusion [5].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00703
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004663
UniProtKB/Swiss-Prot: (23) [show] [UniProt]

References

  1. Casey, P.J. and Seabra, M.C.
    Protein prenyltransferases.
    J. Biol. Chem. 271: 5289-5292 (1996). [PMID: 8621375]
  2. Wilson, A.L., Erdman, R.A., Castellano, F. and Maltese, W.A.
    Prenylation of Rab8 GTPase by type I and type II geranylgeranyl transferases.
    Biochem. J. 333: 497-504 (1998). [PMID: 9677305]
  3. Zhang, H., Seabra, M.C. and Deisenhofer, J.
    Crystal structure of Rab geranylgeranyltransferase at 2.0 Å resolution.
    Structure Fold. Des. 8: 241-251 (2000). [PMID: 10745007]
  4. Thomä, N.H., Niculae, A., Goody, R.S. and Alexandrov, K.
    Double prenylation by RabGGTase can proceed without dissociation of the mono-prenylated intermediate.
    J. Biol. Chem. 276: 48631-48636 (2001). [PMID: 11591706]
  5. Rak, A., Niculae, A., Kalinin, A., Thomä, N.H., Sidorovitch, V., Goody, R.S. and Alexandrov, K.
    In vitro assembly, purification, and crystallization of the Rab geranylgeranyl transferase:substrate complex.
    Protein Expr. Purif. 25: 23-30 (2002). [PMID: 12071695]
  6. Gibbs, R.A.
    Prenyl transfer and the enzymes of terpenoid and steroid biosynthesis.
    In: Sinnott, M. (Ed.) Comprehensive Biological Catalysis. A Mechanistic Reference. vol. 1, Academic Press, San Diego, 1998, 31-118

[EC 2.5.1.60 created 2003]