EC 2.5.1.59 - Protein geranylgeranyltransferase type I

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IntEnz Enzyme Nomenclature
EC 2.5.1.59

Names

Accepted name:
protein geranylgeranyltransferase type I
Other names:
GGTase-I
GGTaseI
Systematic name:
geranylgeranyl-diphosphate:protein-cysteine geranyltransferase

Reaction

Cofactor

Comments:

This enzyme, along with protein farnesyltransferase (EC 2.5.1.58) and protein geranylgeranyltransferase type II (EC 2.5.1.60), constitutes the protein prenyltransferase family of enzymes. Catalyses the formation of a thioether linkage between the C-1 atom of the geranylgeranyl group and a cysteine residue fourth from the C-terminus of the protein. These protein acceptors have the C-terminal sequence CA1A2X, where the terminal residue, X, is preferably leucine; serine, methionine, alanine or glutamine makes the protein a substrate for EC 2.5.1.58. The enzymes are relaxed in specificity for A1, but cannot act if A2 is aromatic. Known targets of this enzyme include most γ-subunits of heterotrimeric G proteins and Ras-related GTPases such as members of the Ras and Rac/Rho families. A zinc metalloenzyme. The Zn2+ is required for peptide, but not for isoprenoid, substrate binding.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00703
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004662
UniProtKB/Swiss-Prot: (19) [show] [UniProt]

References

  1. Casey, P.J. and Seabra, M.C.
    Protein prenyltransferases.
    J. Biol. Chem. 271: 5289-5292 (1996). [PMID: 8621375]
  2. Zhang, F.L. and Casey, P.J.
    Influence of metal ions on substrate binding and catalytic activity of mammalian protein geranylgeranyltransferase type-I.
    Biochem. J. 320: 925-932 (1996). [PMID: 9003382]
  3. Gibbs, R.A.
    Prenyl transfer and the enzymes of terpenoid and steroid biosynthesis.
    In: Sinnott, M. (Ed.) Comprehensive Biological Catalysis. A Mechanistic Reference. vol. 1, Academic Press, San Diego, 1998, 31-118

[EC 2.5.1.59 created 2003]