EC 2.4.1.212 - Hyaluronan synthase

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IntEnz Enzyme Nomenclature
EC 2.4.1.212

Names

Accepted name:
hyaluronan synthase
Other names:
spHAS
seHAS
HAS
Alternating UDP-α-N-acetyl-D-glucosamine:β-D-glucuronosyl-(1→3)-[nascent hyaluronan] 4-N-acetyl-β-D-glucosaminyltransferase and UDP-α-D-glucuronate:N-acetyl-β-D-glucosaminyl-(1→4)-[nascent hyaluronan] 3-β-D-glucuronosyltransferase
Systematic name:
Alternating UDP-N-acetyl-α-D-glucosamine:β-D-glucuronosyl-(1→3)-[nascent hyaluronan] 4-N-acetyl-β-D-glucosaminyltransferase and UDP-α-D-glucuronate:N-acetyl-β-D-glucosaminyl-(1→4)-[nascent hyaluronan] 3-β-D-glucuronosyltransferase (configuration-inverting)

Reactions

Cofactor

Comments:

The enzyme from Streptococcus Group A and Group C requires Mg2+. The enzyme adds GlcNAc to nascent hyaluronan when the non-reducing end is GlcA, but it adds GlcA when the non-reducing end is GlcNAc [3]. The enzyme is highly specific for UDP-GlcNAc and UDP-GlcA; no copolymerization is observed if either is replaced by UDP-Glc, UDP-Gal, UDP-GalNAc or UDP-GalA. Similar enzymes have been found in a variety of organisms.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050501
UniProtKB/Swiss-Prot: (22) [show] [UniProt]

References

  1. DeAngelis, P.L., Papaconstantinou, J. and Weigel, P.H.
    Molecular cloning, identification and sequence of the hyaluronan synthase gene from Group A Streptococcus pyogenes.
    J. Biol. Chem. 268: 19181-19184 (1993). [PMID: 8366070]
  2. Jing, W. and DeAngelis, P.L.
    Dissection of the two transferase activities of the Pasteurella multocida hyaluronan synthase: two active sites exist in one polypeptide.
    Glycobiology 10: 883-889 (2000). [PMID: 10988250]
  3. DeAngelis, P.L.
    Molecular directionality of polysaccharide polymerization by the Pasteurella multocida hyaluronan synthase.
    J. Biol. Chem. 274: 26557-26562 (1993). [PMID: 10473619]
  4. Tlapak-Simmons, V.L., Baron, C.A. and Weigel, P.H.
    Characterization of the purified hyaluronan synthase from Streptococcus equisimilis.
    Biochemistry 43: 9234-9242 (2004). [PMID: 15248781]

[EC 2.4.1.212 created 2001, modified 2007]