EC 2.4.1.150 - N-acetyllactosaminide β-1,6-N-acetylglucosaminyltransferase

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IntEnz Enzyme Nomenclature
EC 2.4.1.150

Names

Accepted name:
N-acetyllactosaminide β-1,6-N-acetylglucosaminyltransferase
Other names:
N-acetylglucosaminyltransferase
Galβ1→4GlcNAc-R β1→6 N-acetylglucosaminyltransferase
uridine diphosphoacetylglucosamine-acetyllactosaminide β1→6-acetylglucosaminyltransferase
UDP-N-acetyl-D-glucosamine:β-D-galactosyl-1,4-N-acetyl-D-glucosaminide β-1,6-N-acetyl-D-glucosaminyltransferase
GCNT2 (gene name)
GCNT3 (gene name)
IGnT
I-branching β1,6-N-acetylglucosaminyltransferase
Systematic name:
UDP-N-acetyl-α-D-glucosamine:β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminide 6-β-N-acetylglucosaminyltransferase (configuration-inverting)

Reactions

Comments:

The enzyme acts on poly-N-acetyllactosamine [glycan chains of β-D-galactosyl-(1→4)-N-acetyl-D-glucosamine units connected by β(1,3) linkages] attached to proteins or lipids. It transfers a GlcNAc residue by β(1,6) linkage to galactosyl residues close to non-reducing terminals, introducing a branching pattern known as I branching.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008109
CAS Registry Number: 85638-40-0
UniProtKB/Swiss-Prot: (15) [show] [UniProt]

References

  1. Van den Eijnden, D.H., Winterwerp, H., Smeeman, P. and Schiphorst, W.E.C.M.
    Novikoff ascites tumor cells contain N-acetyllactosaminide β1→3 and β1→6 N-acetylglucosaminyltransferase activity.
    J. Biol. Chem. 258: 3435-3437 (1983). [PMID: 6219989]
  2. Basu, M., Basu, S.
    Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta 1-3- and beta 1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma.
    J. Biol. Chem. 259: 12557-12562 (1984). [PMID: 6238026]
  3. Piller, F., Cartron, J. P., Maranduba, A., Veyrieres, A., Leroy, Y., Fournet, B.
    Biosynthesis of blood group I antigens. Identification of a UDP-GlcNAc:GlcNAc beta 1-3Gal(-R) beta 1-6(GlcNAc to Gal) N-acetylglucosaminyltransferase in hog gastric mucosa.
    J. Biol. Chem. 259: 13385-13390 (1984). [PMID: 6490658]
  4. Bierhuizen, M. F., Maemura, K., Kudo, S., Fukuda, M.
    Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.
    Glycobiology 5: 417-425 (1995). [PMID: 7579796]
  5. Ujita, M., McAuliffe, J., Suzuki, M., Hindsgaul, O., Clausen, H., Fukuda, M. N., Fukuda, M.
    Regulation of I-branched poly-N-acetyllactosamine synthesis. Concerted actions by I-extension enzyme, I-branching enzyme, and beta1,4-galactosyltransferase I.
    J. Biol. Chem. 274: 9296-9304 (1999). [PMID: 10092606]
  6. Yeh, J. C., Ong, E., Fukuda, M.
    Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.
    J. Biol. Chem. 274: 3215-3221 (1999). [PMID: 9915862]

[EC 2.4.1.150 created 1984 (EC 2.4.1.164 created 1989, incorporated 2016), modified 2017]