EC 2.4.1.14 - Sucrose-phosphate synthase

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IntEnz Enzyme Nomenclature
EC 2.4.1.14

Names

Accepted name:
sucrose-phosphate synthase
Other names:
SPS
UDP-glucose—fructose-phosphate glucosyltransferase
UDPglucose:D-fructose-6-phosphate 2-α-D-glucosyltransferase
sucrose 6-phosphate synthase
sucrose phosphate synthetase
sucrose phosphate-uridine diphosphate glucosyltransferase
sucrosephosphate—UDP glucosyltransferase
uridine diphosphoglucose-fructose phosphate glucosyltransferase
UDP-glucose-fructose-phosphate glucosyltransferase
UDP-glucose:D-fructose-6-phosphate 2-α-D-glucosyltransferase
Systematic name:
UDP-α-D-glucose:D-fructose-6-phosphate 2-α-D-glucosyltransferase (configuration-retaining)

Reaction

Cofactor

Comments:

Requires Mg2+ or Mn2+ for maximal activity [2]. The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates [2]. The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not [2]. While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible [3]. The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , NIST 74 , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0046524
CAS Registry Number: 9030-06-2
UniProtKB/Swiss-Prot: (19) [show] [UniProt]

References

  1. Mendicino, J.
    Sucrose phosphate synthesis in wheat germ and green leaves.
    J. Biol. Chem. 235: 3347-3352 (1960). [PMID: 13769376]
  2. Curatti, L., Folco, E., Desplats, P., Abratti, G., Limones, V., Herrera-Estrella, L. and Salerno, G.
    Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.
    J. Bacteriol. 180: 6776-6779 (1998). [PMID: 9852031]
  3. Huber, S.C. and Huber, J.L.
    Role and regulation of sucrose-phosphate synthase in higher plants.
    Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 431-444 (1996). [PMID: 15012296]
  4. Cumino, A., Curatti, L., Giarrocco, L. and Salerno, G.L.
    Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution.
    FEBS Lett. 517: 19-23 (2002). [PMID: 12062401]
  5. Chua, T.K., Bujnicki, J.M., Tan, T.-C., Huynh, F., Patel, B.K. and Sivaraman, J.
    The structure of sucrose phosphate synthase from Halothermothrix orenii reveals its mechanism of action and binding mode.
    Plant Cell 20: 1059-1072 (2008). [PMID: 18424616]

[EC 2.4.1.14 created 1961, modified 2008]