EC 2.4.1.132 - GDP-Man:Man1GlcNAc2-PP-dolichol α-1,3-mannosyltransferase

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IntEnz Enzyme Nomenclature
EC 2.4.1.132

Names

Accepted name:
GDP-Man:Man1GlcNAc2-PP-dolichol α-1,3-mannosyltransferase
Other names:
Alg2 mannosyltransferase [ambiguous]
ALG2 (gene name) [ambiguous]
mannosyltransferase II
glycolipid 3-α-mannosyltransferase
GDP-mannose:glycolipid 3-α-D-mannosyltransferase
GDP-Man:Man1GlcNAc2-PP-Dol α-1,3-mannosyltransferase
Systematic name:
GDP-D-mannose:D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol 3-α-mannosyltransferase

Reaction

Comments:

The biosynthesis of asparagine-linked glycoproteins utilizes a dolichyl diphosphate-linked glycosyl donor, which is assembled by the series of membrane-bound glycosyltransferases that comprise the dolichol pathway. Alg2 mannosyltransferase from Saccharomyces cerevisiae carries out an α1,3-mannosylation of D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol, followed by an α1,6-mannosylation (cf. EC 2.4.1.257), to form the first branched pentasaccharide intermediate of the dolichol pathway [1,2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004378
CAS Registry Number: 81181-76-2
UniProtKB/Swiss-Prot: (13) [show] [UniProt]

References

  1. Kampf, M., Absmanner, B., Schwarz, M., Lehle, L.
    Biochemical characterization and membrane topology of Alg2 from Saccharomyces cerevisiae as a bifunctional α1,3- and 1,6-mannosyltransferase involved in lipid-linked oligosaccharide biosynthesis.
    J. Biol. Chem. 284: 11900-11912 (2009). [PMID: 19282279]
  2. O'Reilly, M. K., Zhang, G., Imperiali, B.
    In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
    Biochemistry 45: 9593-9603 (2006). [PMID: 16878994]

[EC 2.4.1.132 created 1984, modified 2011, modified 2012]