EC 2.4.1.131 - GDP-Man:Man3GlcNAc2-PP-dolichol α-1,2-mannosyltransferase

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IntEnz Enzyme Nomenclature
EC 2.4.1.131

Names

Accepted name:
GDP-Man:Man3GlcNAc2-PP-dolichol α-1,2-mannosyltransferase
Other names:
GDP-mannose:glycolipid 1,2-α-D-mannosyltransferase
ALG11
ALG11 mannosyltransferase
LEW3 (gene name)
At2G40190 (gene name)
gmd3 (gene name)
galactomannan deficiency protein 3
glycolipid 2-α-mannosyltransferase
GDP-mannose:glycolipid 2-α-D-mannosyltransferase
GDP-Man:Man3GlcNAc2-PP-Dol α-1,2-mannosyltransferase
Systematic name:
GDP-α-D-mannose:D-Man-α-(1→3)-[D-Man-α-(1→6)]-D-Man-β-(1→4)-D-GlcNAc-β-(1→4)-D-GlcNAc-diphosphodolichol 2-α-D-mannosyltransferase

Reaction

Comments:

The biosynthesis of asparagine-linked glycoproteins (N-linked protein glycosylation) utilizes a dolichyl diphosphate-linked glycosyl donor, which is assembled by the series of membrane-bound glycosyltransferases that comprise the dolichol pathway. ALG11 mannosyltransferase from Saccharomyces cerevisiae carries out two sequential steps in the formation of the lipid-linked core oligosaccharide, adding two mannose residues in α(1→2) linkages to the nascent oligosaccharide.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004377
CAS Registry Number: 74506-43-7
UniProtKB/Swiss-Prot: (18) [show] [UniProt]

References

  1. O'Reilly, M. K., Zhang, G., Imperiali, B.
    In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
    Biochemistry 45: 9593-9603 (2006). [PMID: 16878994]
  2. Absmanner, B., Schmeiser, V., Kampf, M., Lehle, L.
    Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae, an α1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide.
    Biochem. J. 426: 205-217 (2010). [PMID: 19929855]
  3. Schutzbach, J.S., Springfield, J.D. and Jensen, J.W.
    The biosynthesis of oligosaccharide-lipids. Formation of an α-1,2-mannosyl-mannose linkage.
    J. Biol. Chem. 255: 4170-4175 (1980). [PMID: 6154707]

[EC 2.4.1.131 created 1984, modified 2011, modified 2012]