EC 2 - Transferases
EC 2.3 - Acyltransferases
EC 2.3.3 - Acyl groups converted into alkyl groups on transfer
EC 2.3.3.14 - Homocitrate synthase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 2.3.3.14
Names
Accepted name:
homocitrate synthase
Other
names:
2-hydroxybutane-1,2,4-tricarboxylate 2-oxoglutarate-lyase (CoA-acetylating)
acetyl-coenzyme A:2-ketoglutarate C-acetyl transferase
homocitrate synthetase
HCS
acetyl-coenzyme A:2-ketoglutarate C-acetyl transferase
homocitrate synthetase
HCS
Systematic name:
acetyl-CoA:2-oxoglutarate C-acetyltransferase (thioester-hydrolysing, carboxymethyl forming)
Reaction
- acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
Comments:
Belongs in the α-aminoadipate pathway of lysine synthesis, along with EC 4.2.1.36, homoaconitate hydratase. The enzyme also acts with oxaloacetate as substrate, but more slowly [2,3].
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
DIAGRAM
,
DIAGRAM
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
,
MetaCyc
,
UniPathway
Protein domains and families:
PROSITE:PDOC00643
,
PROSITE:PDOC50991
Gene Ontology:
GO:0004410
CAS Registry Number:
9075-60-9
References
-
Enzymatic formation of homocitric acid, an intermediate in lysine biosynthesis.Biochem. Biophys. Res. Commun. 14 : 262-267 (1964). [PMID: 5836514]
-
Characterization of bacterial homocitrate synthase involved in lysine biosynthesis.FEBS Lett. 522 : 35-40 (2002). [PMID: 12095615]
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Kinetic mechanism of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae.Biochemistry 43 : 11790-11795 (2004). [PMID: 15362863]
[EC 2.3.3.14 created 1972 as EC 4.1.3.21, transferred 2002 to EC 2.3.3.14]