EC 2.3.3.14 - Homocitrate synthase

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IntEnz Enzyme Nomenclature
EC 2.3.3.14

Names

Accepted name:
homocitrate synthase
Other names:
2-hydroxybutane-1,2,4-tricarboxylate 2-oxoglutarate-lyase (CoA-acetylating)
acetyl-coenzyme A:2-ketoglutarate C-acetyl transferase
homocitrate synthetase
HCS
Systematic name:
acetyl-CoA:2-oxoglutarate C-acetyltransferase (thioester-hydrolysing, carboxymethyl forming)

Reaction

Comments:

Belongs in the α-aminoadipate pathway of lysine synthesis, along with EC 4.2.1.36, homoaconitate hydratase. The enzyme also acts with oxaloacetate as substrate, but more slowly [2,3].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00643 , PROSITE:PDOC50991
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004410
CAS Registry Number: 9075-60-9
UniProtKB/Swiss-Prot: (34) [show] [UniProt]

References

  1. Strassman, M. and Ceci, L.N.
    Enzymatic formation of homocitric acid, an intermediate in lysine biosynthesis.
    Biochem. Biophys. Res. Commun. 14: 262-267 (1964). [PMID: 5836514]
  2. Wulandari, A.P., Miyazaki, J., Kobashi, N., Nishiyama, M., Hoshino, T. and Yamane, H.
    Characterization of bacterial homocitrate synthase involved in lysine biosynthesis.
    FEBS Lett. 522: 35-40 (2002). [PMID: 12095615]
  3. Andi, B., West, A.H. and Cook, P.F.
    Kinetic mechanism of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae.
    Biochemistry 43: 11790-11795 (2004). [PMID: 15362863]

[EC 2.3.3.14 created 1972 as EC 4.1.3.21, transferred 2002 to EC 2.3.3.14]