EC 2.3.1.97 - Glycylpeptide N-tetradecanoyltransferase

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IntEnz Enzyme Nomenclature
EC 2.3.1.97

Names

Accepted name:
glycylpeptide N-tetradecanoyltransferase
Other names:
myristoyl-CoA-protein N-myristoyltransferase
myristoyl-coenzyme A:protein N-myristoyl transferase
myristoylating enzymes
peptide N-myristoyltransferase
protein N-myristoyltransferase
peptide N-tetradecanoyltransferase
tetradecanoyl-CoA:glycylpeptide N-tetradecanoyltransferase
Systematic name:
tetradecanoyl-CoA:N-terminal glycyl-[protein] N-tetradecanoyltransferase

Reaction

Comments:

The enzyme catalyses the transfer of myristic acid from myristoyl-CoA to the amino group of the N-terminal glycine residue in a variety of eukaryotic proteins. It uses an ordered Bi Bi reaction in which myristoyl-CoA binds to the enzyme prior to the binding of the peptide substrate, and CoA release precedes the release of the myristoylated peptide. The enzyme from yeast is profoundly affected by amino acids further from the N-terminus, and is particularly stimulated by a serine residue at position 5.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00752
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004379
CAS Registry Number: 110071-61-9
UniProtKB/Swiss-Prot: (35) [show] [UniProt]

References

  1. Guertin, D., Gris-Miron, L. and Riendeau, D.
    Identification of a 51-kilodalton polypeptide fatty acyl chain acceptor in soluble extracts from mouse cardiac tissue.
    Biochem. Cell Biol. 64: 1249-1255 (1986). [PMID: 3566958]
  2. Heuckeroth, R.O., Towler, D.A., Adams, S.P., Glaser, L. and Gordon, J.I.
    11-(Ethylthio)undecanoic acid. A myristic acid analogue of altered hydrophobicity which is functional for peptide N-myristoylation with wheat germ and yeast acyltransferase.
    J. Biol. Chem. 263: 2127-2133 (1988). [PMID: 3123489]
  3. Towler, D. A., Adams, S. P., Eubanks, S. R., Towery, D. S., Jackson-Machelski, E., Glaser, L., Gordon, J. I.
    Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase.
    Proc. Natl. Acad. Sci. U.S.A. 84: 2708-2712 (1987). [PMID: 3106975]
  4. McIlhinney, R. A., Young, K., Egerton, M., Camble, R., White, A., Soloviev, M.
    Characterization of human and rat brain myristoyl-CoA:protein N-myristoyltransferase: evidence for an alternative splice variant of the enzyme.
    Biochem. J. 333 ( Pt 3): 491-495 (1998). [PMID: 9677304]
  5. Farazi, T. A., Waksman, G., Gordon, J. I.
    Structures of Saccharomyces cerevisiae N-myristoyltransferase with bound myristoylCoA and peptide provide insights about substrate recognition and catalysis.
    Biochemistry 40: 6335-6343 (2001). [PMID: 11371195]

[EC 2.3.1.97 created 1989, modified 1990]