EC 2.3.1.47 - 8-amino-7-oxononanoate synthase

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IntEnz Enzyme Nomenclature
EC 2.3.1.47

Names

Accepted name:
8-amino-7-oxononanoate synthase
Other names:
7-keto-8-aminopelargonic acid synthetase
7-keto-8-aminopelargonic synthetase
8-amino-7-oxopelargonate synthase
8-amino-7-ketopelargonate synthase
AONS
7-KAP synthetase
bioF (gene name)
Systematic name:
6-carboxyhexanoyl-[acyl-carrier protein]:L-alanine C-carboxyhexanoyltransferase (decarboxylating)

Reaction

Cofactor

Comments:

A pyridoxal-phosphate protein. The enzyme catalyses the decarboxylative condensation of L-alanine and pimeloyl-[acyl-carrier protein], a key step in the pathway for biotin biosynthesis. Pimeloyl-CoA can be used with lower efficiency [5].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00518
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008710
CAS Registry Number: 9075-61-0
UniProtKB/Swiss-Prot: (303) [show] [UniProt]

References

  1. Eisenberg, M.A. and Star, C.
    Synthesis of 7-oxo-8-aminopelargonic acid, a biotin vitamer, in cell-free extracts of Escherichia coli biotin auxotrophs.
    J. Bacteriol. 96: 1291-1297 (1968). [PMID: 4879561]
  2. Alexeev, D., Alexeeva, M., Baxter, R.L., Campopiano, D.J., Webster, S.P. and Sawyer, L.
    The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme.
    J. Mol. Biol. 284: 401-419 (1998). [PMID: 9813126]
  3. Ploux, O., Breyne, O., Carillon, S. and Marquet, A.
    Slow-binding and competitive inhibition of 8-amino-7-oxopelargonate synthase, a pyridoxal-5'-phosphate-dependent enzyme involved in biotin biosynthesis, by substrate and intermediate analogs. Kinetic and binding studies.
    Eur. J. Biochem. 259: 63-70 (1999). [PMID: 9914476]
  4. Webster, S.P., Alexeev, D., Campopiano, D.J., Watt, R.M., Alexeeva, M., Sawyer, L. and Baxter, R.L.
    Mechanism of 8-amino-7-oxononanoate synthase: spectroscopic, kinetic, and crystallographic studies.
    Biochemistry 39: 516-528 (2000). [PMID: 10642176]
  5. Lin, S., Hanson, R. E., Cronan, J. E.
    Biotin synthesis begins by hijacking the fatty acid synthetic pathway.
    Nat. Chem. Biol. 6: 682-688 (2010). [PMID: 20693992]

[EC 2.3.1.47 created 1976, modified 2013]