EC - Diphthine synthase

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IntEnz Enzyme Nomenclature


Accepted name:
diphthine synthase
Other names:
S-adenosyl-L-methionine:elongation factor 2 methyltransferase [ambiguous]
diphthine methyltransferase [ambiguous]
S-adenosyl-L-methionine:2-(3-carboxy-3-aminopropyl)-L-histidine methyltransferase [ambiguous]
Dph5 [ambiguous]
Systematic name:
S-adenosyl-L-methionine:2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] methyltransferase (diphthine-[translation elongation factor 2]-forming)



This archaeal enzyme produces the trimethylated product diphthine, which is converted into diphthamide by EC, diphthine—ammonia ligase. Different from the eukaryotic enzyme, which produces diphthine methyl ester (cf. EC In the archaeon Pyrococcus horikoshii the enzyme acts on His600 of elongation factor 2.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004164
CAS Registry Number: 114514-25-9
UniProtKB/Swiss-Prot: (51) [show] [UniProt]


  1. Zhu, X., Kim, J., Su, X., Lin, H.
    Reconstitution of diphthine synthase activity in vitro.
    Biochemistry 49: 9649-9657 (2010). [PMID: 20873788]

[EC created 1990, modified 2013, modified 2015]