EC - Site-specific DNA-methyltransferase (cytosine-N4-specific)

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IntEnz Enzyme Nomenclature


Accepted name:
site-specific DNA-methyltransferase (cytosine-N4-specific)
Other names:
m4C-forming MTase
modification methylase
restriction-modification system
N4-cytosine-specific DNA methylase
S-adenosyl-L-methionine:DNA-cytosine 4-N-methyltransferase
Systematic name:
S-adenosyl-L-methionine:DNA-cytosine N4-methyltransferase



This is a large group of enzymes, most of which, with enzymes of similar site specificity listed as EC type 1 site-specific deoxyribonuclease, EC type II site-specific deoxyribonuclease or EC type III site-specific deoxyribonuclease, form so-called 'restriction-modification systems'. A complete listing of all of these enzymes has been produced by R.J. Roberts and is available on-line at the REBASE website.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00088
Structural data: CSA , EC2PDB
Gene Ontology: GO:0015667
UniProtKB/Swiss-Prot: (22) [show] [UniProt]


  1. Kessler, C. and Manta, V.
    Specificity of restriction endonucleases and DNA modification methyltransferases. A review (Edition 3).
    Gene 92: 1-248 (1990). [PMID: 2172084]
  2. Klimasauskas, S., Timinskas, A., Menkevicius, S., Butkiene, D., Butkus, V. and Janulaitis, A.
    Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases.
    Nucleic Acids Res. 17: 9823-9832 (1989). [PMID: 2690010]
  3. Roberts, R.J.
    Restriction enzymes and their isoschizomers.
    Nucleic Acids Res. 18: 2331-2365 (1990). [PMID: 2159140]
  4. Yuan, R.
    Structure and mechanism of multifunctional restriction endonucleases.
    Annu. Rev. Biochem. 50: 285-319 (1981). [PMID: 6267988]
  5. Roberts, R.J. and Macelis, D.
    REBASE—restriction enzymes and methylases.
    Nucleic Acids Res. 29: 268-269 (2001). [PMID: 11125108]
  6. Roberts, R.J., Belfort, M., Bestor, T., Bhagwat, A.S., Bickle, T.A., Bitinaite, J., Blumenthal, R.M., Degtyarev, S.Kh., Dryden, D.T., Dybvig, K., Firman, K., Gromova, E.S., Gumport, R.I., Halford, S.E., Hattman, S., Heitman, J., Hornby, D.P., Janulaitis, A., Jeltsch, A., Josephsen, J., Kiss, A., Klaenhammer, T.R., Kobayashi, I., Kong, H., Krüger, D.H., Lacks, S., Marinus, M.G., Miyahara, M., Morgan, R.D., Murray, N.E., Nagaraja, V., Piekarowicz, A., Pingoud, A., Raleigh, E., Rao, D.N., Reich, N., Repin, V.E., Selker, E.U., Shaw, P.C., Stein, D.C., Stoddard, B.L., Szybalski, W., Trautner, T.A., Van Etten, J.L., Vitor, J.M., Wilson, G.G. and Xu, S.Y.
    A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.
    Nucleic Acids Res. 31: 1805-1812 (2003). [PMID: 12654995]

[EC created 1992]