EC 1.6.3.1 - NAD(P)H oxidase (H2O2-forming)

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IntEnz Enzyme Nomenclature
EC 1.6.3.1

Names

Accepted name:
NAD(P)H oxidase (H2O2-forming)
Other names:
NADPH oxidase
THOX2
ThOX
dual oxidase
p138tox
thyroid NADPH oxidase
thyroid oxidase
thyroid oxidase 2
NAD(P)H:oxygen oxidoreductase
NAD(P)H oxidase
Systematic name:
NAD(P)H:oxygen oxidoreductase (H2O2-forming)

Reactions

Cofactors

Comments:

Requires FAD, heme and calcium. When calcium is present, this transmembrane glycoprotein generates H2O2 by transfering electrons from intracellular NAD(P)H to extracellular molecular oxygen. The electron bridge within the enzyme contains one molecule of FAD and probably two heme groups. This flavoprotein is expressed at the apical membrane of thyrocytes, and provides H2O2 for the thyroid peroxidase-catalysed biosynthesis of thyroid hormones.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00394
Structural data: CSA , EC2PDB
Gene Ontology: GO:0016174
CAS Registry Number: 77106-92-4
UniProtKB/Swiss-Prot:

References

  1. Moreno, J.C., Bikker, H., Kempers, M.J., van Trotsenburg, A.S., Baas, F., de Vijlder, J.J., Vulsma, T. and Ris-Stalpers, C.
    Inactivating mutations in the gene for thyroid oxidase 2 (THOX2) and congenital hypothyroidism.
    N. Engl. J. Med. 347: 95-102 (2002). [PMID: 12110737]
  2. De Deken, X., Wang, D., Dumont, J.E. and Miot, F.
    Characterization of ThOX proteins as components of the thyroid H2O2-generating system.
    Exp. Cell Res. 273: 187-196 (2002). [PMID: 11822874]
  3. De Deken, X., Wang, D., Many, M.C., Costagliola, S., Libert, F., Vassart, G., Dumont, J.E. and Miot, F.
    Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family.
    J. Biol. Chem. 275: 23227-23233 (2000). [PMID: 10806195]
  4. Dupuy, C., Ohayon, R., Valent, A., Noel-Hudson, M.S., Deme, D. and Virion, A
    Purification of a novel flavoprotein involved in the thyroid NADPH oxidase. Cloning of the porcine and human cDNAs.
    J. Biol. Chem. 274: 37265-37269 (1999). [PMID: 10601291]
  5. Leseney, A.M., Deme, D., Legue, O., Ohayon, R., Chanson, P., Sales, J.P., Pires de Carvalho, D., Dupuy, C. and Virion, A.
    Biochemical characterization of a Ca2+/NAD(P)H-dependent H2O2 generator in human thyroid tissue.
    Biochimie 81: 373-380 (1999). [PMID: 10401672]
  6. Dupuy, C., Virion, A., Ohayon, R., Kaniewski, J., Deme, D. and Pommier, J.
    Mechanism of hydrogen peroxide formation catalyzed by NADPH oxidase in thyroid plasma membrane.
    J. Biol. Chem. 266: 3739-3743 (1991). [PMID: 1995628]

[EC 1.6.3.1 created 2003, modified 2012]