EC 1.14.20.1 - Deacetoxycephalosporin-C synthase

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IntEnz Enzyme Nomenclature
EC 1.14.20.1

Names

Accepted name:
deacetoxycephalosporin-C synthase
Other names:
DAOCS
penicillin N expandase
DAOC synthase
Systematic name:
penicillin-N,2-oxoglutarate:oxygen oxidoreductase (ring-expanding)

Reaction

Comments:

Forms part of the penicillin biosynthesis pathway.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00165 , PROSITE:PDOC51471
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050599
CAS Registry Number: 85746-10-7
UniProtKB/Swiss-Prot:

References

  1. Cantwell, C., Beckmann, R., Whiteman, P., Queener, S.W. and Abraham, E.P.
    Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants - construction of a new fungal biosynthetic-pathway.
    Proc. R. Soc. Lond. B Biol. Sci. 248: 283-289 (1992). [PMID: 1354366]
  2. Lee, H.J., Lloyd, M.D., Harlos, K., Clifton, I.J., Baldwin, J.E. and Schofield, C.J.
    Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).
    J. Mol. Biol. 308: 937-948 (2001). [PMID: 11352583]
  3. Yeh, W.K., Ghag, S.K. and Queener, S.W.
    Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C. Function, evolution, refolding, and enzyme engineering.
    Ann. N.Y. Acad. Sci. 672: 396-408 (1992).
  4. Valegård, K., van Scheltinga, A.C.T., Lloyd, M.D., Hara, T., Ramaswamy, S., Perrakis, A., Thompson, A., Lee, H.-J., Baldwin, J.E., Schofield, C.J., Hajdu, J. and Andersson, I.
    Structure of a cephalosporin synthase.
    Nature 394: 805-809 (1998). [PMID: 9723623]
  5. Dotzlaf, J.E. and Yeh, W.K.
    Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus.
    J. Biol. Chem. 264: 10219-10227 (1989). [PMID: 2656705]

[EC 1.14.20.1 created 2002]