EC 1.1.1.261 - sn-glycerol-1-phosphate dehydrogenase

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IntEnz Enzyme Nomenclature
EC 1.1.1.261

Names

Accepted name:
sn-glycerol-1-phosphate dehydrogenase
Other names:
glycerol-1-phosphate dehydrogenase [NAD(P)+]
sn-glycerol-1-phosphate:NAD+ 2-oxidoreductase
G-1-P dehydrogenase
Gro1PDH
AraM
Systematic name:
sn-glycerol-1-phosphate:NAD(P)+ 2-oxidoreductase

Reactions

Cofactor

Comments:

This enzyme is found primarily as a Zn2+-dependent form in archaea but a Ni2+-dependent form has been found in Gram-positive bacteria [7]. The Zn2+-dependent metalloenzyme is responsible for the formation of archaea-specific sn-glycerol-1-phosphate, the first step in the biosynthesis of polar lipids in archaea^. It is the enantiomer of sn-glycerol 3-phosphate, the form of glycerophosphate found in bacteria and eukaryotes. The other enzymes involved in the biosynthesis of polar lipids in archaea are EC 2.5.1.41 (phosphoglycerol geranylgeranyltransferase) and EC 2.5.1.42 (geranylgeranylglycerol-phosphate geranylgeranyltransferase), which together alkylate the hydroxy groups of glycerol 1-phosphate to give unsaturated archaetidic acid, which is acted upon by EC 2.7.7.67 (CDP-archaeol synthase) to form CDP-unsaturated archaeol. The final step in the pathway involves the addition of L-serine, with concomitant removal of CMP, leading to the production of unsaturated archaetidylserine [5]. Activity of the enzyme is stimulated by K+ [2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050492
CAS Registry Number: 204594-18-3
UniProtKB/Swiss-Prot: (68) [show] [UniProt]

References

  1. Nishihara, M. and Koga, Y.
    sn-glycerol-1-phosphate dehydrogenase in Methanobacterium thermoautotrophicum: key enzyme in biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of archaebacteria.
    J. Biochem. 117: 933-935 (1995). [PMID: 8586635]
  2. Nishihara, M. and Koga, Y.
    Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of archaea.
    J. Biochem. 122: 572-576 (1997). [PMID: 9348086]
  3. Nishihara, M., Koga, Y.
    Erratum report: Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea.
    J. Biochem. 123: 194 (1998).
  4. Koga, Y., Kyuragi, T., Nishihara, M. and Sone, N.
    Did archaeal and bacterial cells arise independently from noncellular precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines of descent.
    J. Mol. Evol. 46: 54-63 (1998). [PMID: 9419225]
  5. Morii, H., Nishihara, M. and Koga, Y.
    CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase in the methanogenic archaeon Methanothermobacter thermoautotrophicus.
    J. Biol. Chem. 275: 36568-36574 (2000). [PMID: 10960477]
  6. Han, J.S. and Ishikawa, K.
    Active site of Zn2+-dependent sn-glycerol-1-phosphate dehydrogenase from Aeropyrum pernix K1.
    Archaea 1: 311-317 (2005). [PMID: 15876564]
  7. Guldan, H., Sterner, R. and Babinger, P.
    Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni2+-dependent AraM from Bacillus subtilis.
    Biochemistry 47: 7376-7384 (2008). [PMID: 18558723]

[EC 1.1.1.261 created 2000, modified 2009]