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What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 19022416

Journal: J Proteomics (1874-3919)

Year of Publication: 2009

Title: Proteomics analysis of immuno-precipitated synaptic protein complexes.

Author List: Klemmer P., Smit AB., Li KW.

Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Experiment (13 interactions)

Accession: EBI-3647574

Name: klemmer-2009-1

Host organism: Mus musculus brain cortex

Organism: Mus musculus brain cortex

Accession: EBI-973600

Name: mouse-brain-cortex

Description: Mus musculus brain cortex

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse

Interaction Detection Method: anti bait coip

Interaction detection method: anti bait coip
Accession: EBI-976

Name: anti bait coip

Description: anti bait coimmunoprecipitation

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0006
-
identity
pubmed
7708014
-
primary-reference


Annotations:
Topic
Text
definition
A specific antibody for the molecule of interest (bait) is available, this is used to generate a high affinity resin to capture the endogenous bait present in a sample.


Participant Identification Method: sequence tag

Participant identification method: sequence tag
Accession: EBI-81

Name: sequence tag

Description: sequence tag identification

Cross References:
Database
Identifier
Secondary identifier
Qualifier
pubmed
11805837
-
see-also
psi-mi
MI:0102
-
identity
pubmed
11752590
-
method reference
pubmed
10967324
-
primary-reference


Annotations:
Topic
Text
definition
This approach leads to protein identification by combining mass measurement and short amino acid sequence information obtained by tandem mass spectrometry. This information is then used to automatically find the best match in a sequence database. A mixture of peptides derived from a protease digestion is analysed by nanoelectrospray LC-MS/MS (Liquid Chromatography Tandem Mass Spectrometer or nanoESI MS/MS) mass spectrometry. Electrospray mass spectrometry cannot be applied to dilute samples and is affected by high salt. As a consequence peptides, normally extracted from acrylamide gels by in situ proteolysis, are desalted and concentrated on a microcolumn followed by elution into a capillary used for nanoelectrospray tandem mass spectrometry. A first mass spectrum (Normal mass spectrum or Q1 mass spectrum) gives information about the masses of all the peptides. Peptides observed in the normal mass spectrum are isolated in turn and dissociated into fragments by collision with gas molecules within the mass spectrometer. Some of the fragments obtained from a peptide constitute a nested set, differing by one amino acid, and the mass difference between them allows assignment of a partial sequence. The masses of the fragments define the position of the partial sequence in the peptide. Together with the cleavage specificity of the protease used to cleave the protein, and mass information such sequence tag provides much higher search specificity to match the a database entry. The procedure is repeated with several peptides from the digest, resulting in multiple identifications of the same protein or identification of several proteins from the peptide mixture. Unknown proteins can easily be identified by using the high specificity of the peptide sequence tag for searches in most sequence databases including EST or genome databases.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-
-

Annotations:

Topic
Text
imex curation
Mouse cortex is homogenised in 2% triton centrifuge to obtain synatosomal mass pellet at 16,000. this was subject to immunoprecipitation. The proteins that were pulled down were then subject to tryptic digest and analyzed by MaldiTof MS/MS.
Sets of contaminants due to e.g. non-specific binding to beads such as serum proteins, keratin, mitochondrial and metabolic proteins, and several other non-neuronal proteins, such as myelin basic protein occurred in structurally distinct protein complexes, and appeared also in IP samples from which the antibody used failed to pull down the target protein. All of these proteins were not considered as synaptic proteins and were excluded from further analysis.
Only protein-protein interactions
-

Interaction

Accession: EBI-3647771

Name: git1-actg1

Description: -

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0914
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-

Annotations:

Topic
Text
Table 7
Ubiquitin was found to be part of the complex but no single unique AC could be associated with it, hence, this protein was not included in the complex.

Participants (14)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
1
EBI-645933
 logo
dastyLogo
G protein-coupled receptor kinase-interactor 1
GRK-interacting protein 1
Git1
ARF GTPase-activating protein GIT1
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally treated to capture its interacting partners.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: ARF GTPase-activating protein GIT1

Participant: ARF GTPase-activating protein GIT1

Accession: EBI-3647796

Name: EBI-645933

P
Participant: ARF GTPase-activating protein GIT1

Participant: ARF GTPase-activating protein GIT1

Accession: EBI-3647796

Name: EBI-645933

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: ARF GTPase-activating protein GIT1

Participant: ARF GTPase-activating protein GIT1

Accession: EBI-3647796

Name: EBI-645933

Stoichiometry: 0.0

F
Participant: ARF GTPase-activating protein GIT1

Participant: ARF GTPase-activating protein GIT1

Accession: EBI-3647796

Name: EBI-645933

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: ARF GTPase-activating protein GIT1

Participant: ARF GTPase-activating protein GIT1

Accession: EBI-3647796

Name: EBI-645933

Confidences:

Type
Value
No records found.

2
EBI-3647772
 logo
dastyLogo
Mfap1
Microfibrillar-associated protein 1
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Microfibrillar-associated protein 1

Participant: Microfibrillar-associated protein 1

Accession: EBI-3647778

Name: EBI-3647772

P
Participant: Microfibrillar-associated protein 1

Participant: Microfibrillar-associated protein 1

Accession: EBI-3647778

Name: EBI-3647772

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Microfibrillar-associated protein 1

Participant: Microfibrillar-associated protein 1

Accession: EBI-3647778

Name: EBI-3647772

Stoichiometry: 0.0

F
Participant: Microfibrillar-associated protein 1

Participant: Microfibrillar-associated protein 1

Accession: EBI-3647778

Name: EBI-3647772

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Microfibrillar-associated protein 1

Participant: Microfibrillar-associated protein 1

Accession: EBI-3647778

Name: EBI-3647772

Confidences:

Type
Value
No records found.

3
EBI-775592
 logo
dastyLogo
p140Cap
SNAP-25-interacting protein
Srcin1

[+3]
SRC kinase signaling inhibitor 1
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: SRC kinase signaling inhibitor 1

Participant: SRC kinase signaling inhibitor 1

Accession: EBI-3647779

Name: EBI-775592

P
Participant: SRC kinase signaling inhibitor 1

Participant: SRC kinase signaling inhibitor 1

Accession: EBI-3647779

Name: EBI-775592

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: SRC kinase signaling inhibitor 1

Participant: SRC kinase signaling inhibitor 1

Accession: EBI-3647779

Name: EBI-775592

Stoichiometry: 0.0

F
Participant: SRC kinase signaling inhibitor 1

Participant: SRC kinase signaling inhibitor 1

Accession: EBI-3647779

Name: EBI-775592

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: SRC kinase signaling inhibitor 1

Participant: SRC kinase signaling inhibitor 1

Accession: EBI-3647779

Name: EBI-775592

Confidences:

Type
Value
No records found.

4
EBI-397460
 logo
dastyLogo
Calm1
Calm
Cam1

[+7]
Calmodulin
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Calmodulin

Participant: Calmodulin

Accession: EBI-3647780

Name: EBI-397460

P
Participant: Calmodulin

Participant: Calmodulin

Accession: EBI-3647780

Name: EBI-397460

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Calmodulin

Participant: Calmodulin

Accession: EBI-3647780

Name: EBI-397460

Stoichiometry: 0.0

F
Participant: Calmodulin

Participant: Calmodulin

Accession: EBI-3647780

Name: EBI-397460

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Calmodulin

Participant: Calmodulin

Accession: EBI-3647780

Name: EBI-397460

Confidences:

Type
Value
No records found.

5
EBI-3647776
 logo
dastyLogo
Afg3l2
AFG3-like protein 2
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: AFG3-like protein 2

Participant: AFG3-like protein 2

Accession: EBI-3647781

Name: EBI-3647776

P
Participant: AFG3-like protein 2

Participant: AFG3-like protein 2

Accession: EBI-3647781

Name: EBI-3647776

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: AFG3-like protein 2

Participant: AFG3-like protein 2

Accession: EBI-3647781

Name: EBI-3647776

Stoichiometry: 0.0

F
Participant: AFG3-like protein 2

Participant: AFG3-like protein 2

Accession: EBI-3647781

Name: EBI-3647776

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: AFG3-like protein 2

Participant: AFG3-like protein 2

Accession: EBI-3647781

Name: EBI-3647776

Confidences:

Type
Value
No records found.

6
EBI-351301
 logo
dastyLogo
Actg1
Actg
Gamma-actin
Actin, cytoplasmic 2
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Actin, cytoplasmic 2

Participant: Actin, cytoplasmic 2

Accession: EBI-3647782

Name: EBI-351301

P
Participant: Actin, cytoplasmic 2

Participant: Actin, cytoplasmic 2

Accession: EBI-3647782

Name: EBI-351301

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Actin, cytoplasmic 2

Participant: Actin, cytoplasmic 2

Accession: EBI-3647782

Name: EBI-351301

Stoichiometry: 0.0

F
Participant: Actin, cytoplasmic 2

Participant: Actin, cytoplasmic 2

Accession: EBI-3647782

Name: EBI-351301

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Actin, cytoplasmic 2

Participant: Actin, cytoplasmic 2

Accession: EBI-3647782

Name: EBI-351301

Confidences:

Type
Value
No records found.

7
EBI-2553564
 logo
dastyLogo
Tubb4b
Tubb2c
Tubulin beta-2C chain
Tubulin beta-4B chain
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Tubulin beta-4B chain

Participant: Tubulin beta-4B chain

Accession: EBI-3647784

Name: EBI-2553564

P
Participant: Tubulin beta-4B chain

Participant: Tubulin beta-4B chain

Accession: EBI-3647784

Name: EBI-2553564

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Tubulin beta-4B chain

Participant: Tubulin beta-4B chain

Accession: EBI-3647784

Name: EBI-2553564

Stoichiometry: 0.0

F
Participant: Tubulin beta-4B chain

Participant: Tubulin beta-4B chain

Accession: EBI-3647784

Name: EBI-2553564

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Tubulin beta-4B chain

Participant: Tubulin beta-4B chain

Accession: EBI-3647784

Name: EBI-2553564

Confidences:

Type
Value
No records found.

8
EBI-911297
 logo
dastyLogo
Calcium regulator SV2A
Sv2a
Kiaa0736

[+1]
Synaptic vesicle glycoprotein 2A
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Synaptic vesicle glycoprotein 2A

Participant: Synaptic vesicle glycoprotein 2A

Accession: EBI-3647785

Name: EBI-911297

P
Participant: Synaptic vesicle glycoprotein 2A

Participant: Synaptic vesicle glycoprotein 2A

Accession: EBI-3647785

Name: EBI-911297

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Synaptic vesicle glycoprotein 2A

Participant: Synaptic vesicle glycoprotein 2A

Accession: EBI-3647785

Name: EBI-911297

Stoichiometry: 0.0

F
Participant: Synaptic vesicle glycoprotein 2A

Participant: Synaptic vesicle glycoprotein 2A

Accession: EBI-3647785

Name: EBI-911297

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Synaptic vesicle glycoprotein 2A

Participant: Synaptic vesicle glycoprotein 2A

Accession: EBI-3647785

Name: EBI-911297

Confidences:

Type
Value
No records found.

9
EBI-3647590
 logo
dastyLogo
Tuba1c
Tuba6
Alpha-tubulin 6

[+2]
Tubulin alpha-1C chain
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Tubulin alpha-1C chain

Participant: Tubulin alpha-1C chain

Accession: EBI-3647786

Name: EBI-3647590

P
Participant: Tubulin alpha-1C chain

Participant: Tubulin alpha-1C chain

Accession: EBI-3647786

Name: EBI-3647590

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Tubulin alpha-1C chain

Participant: Tubulin alpha-1C chain

Accession: EBI-3647786

Name: EBI-3647590

Stoichiometry: 0.0

F
Participant: Tubulin alpha-1C chain

Participant: Tubulin alpha-1C chain

Accession: EBI-3647786

Name: EBI-3647590

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Tubulin alpha-1C chain

Participant: Tubulin alpha-1C chain

Accession: EBI-3647786

Name: EBI-3647590

Confidences:

Type
Value
No records found.

10
EBI-698498
 logo
dastyLogo
PIP2-dependent ARF1 GAP
ADP-ribosylation factor-directed GTPase-activating protein 1
Development and differentiation-enhancing factor 1

[+5]
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Accession: EBI-3647787

Name: EBI-698498

P
Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Accession: EBI-3647787

Name: EBI-698498

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Accession: EBI-3647787

Name: EBI-698498

Stoichiometry: 0.0

F
Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Accession: EBI-3647787

Name: EBI-698498

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Participant: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Accession: EBI-3647787

Name: EBI-698498

Confidences:

Type
Value
No records found.

11
EBI-3647663
 logo
dastyLogo
Lyz2
Lyz
Lyzs

[+2]
Lysozyme C-2
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Lysozyme C-2

Participant: Lysozyme C-2

Accession: EBI-3647788

Name: EBI-3647663

P
Participant: Lysozyme C-2

Participant: Lysozyme C-2

Accession: EBI-3647788

Name: EBI-3647663

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Lysozyme C-2

Participant: Lysozyme C-2

Accession: EBI-3647788

Name: EBI-3647663

Stoichiometry: 0.0

F
Participant: Lysozyme C-2

Participant: Lysozyme C-2

Accession: EBI-3647788

Name: EBI-3647663

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Lysozyme C-2

Participant: Lysozyme C-2

Accession: EBI-3647788

Name: EBI-3647663

Confidences:

Type
Value
No records found.

12
EBI-457317
 logo
dastyLogo
p21-activated kinase 3
Beta-PAK
CDC42/RAC effector kinase PAK-B

[+4]
Serine/threonine-protein kinase PAK 3
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Serine/threonine-protein kinase PAK 3

Participant: Serine/threonine-protein kinase PAK 3

Accession: EBI-3647789

Name: EBI-457317

P
Participant: Serine/threonine-protein kinase PAK 3

Participant: Serine/threonine-protein kinase PAK 3

Accession: EBI-3647789

Name: EBI-457317

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Serine/threonine-protein kinase PAK 3

Participant: Serine/threonine-protein kinase PAK 3

Accession: EBI-3647789

Name: EBI-457317

Stoichiometry: 0.0

F
Participant: Serine/threonine-protein kinase PAK 3

Participant: Serine/threonine-protein kinase PAK 3

Accession: EBI-3647789

Name: EBI-457317

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Serine/threonine-protein kinase PAK 3

Participant: Serine/threonine-protein kinase PAK 3

Accession: EBI-3647789

Name: EBI-457317

Confidences:

Type
Value
No records found.

13
EBI-3647774
 logo
dastyLogo
Pum1
Kiaa0099
Pumilio homolog 1
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Pumilio homolog 1

Participant: Pumilio homolog 1

Accession: EBI-3647790

Name: EBI-3647774

P
Participant: Pumilio homolog 1

Participant: Pumilio homolog 1

Accession: EBI-3647790

Name: EBI-3647774

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Pumilio homolog 1

Participant: Pumilio homolog 1

Accession: EBI-3647790

Name: EBI-3647774

Stoichiometry: 0.0

F
Participant: Pumilio homolog 1

Participant: Pumilio homolog 1

Accession: EBI-3647790

Name: EBI-3647774

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Pumilio homolog 1

Participant: Pumilio homolog 1

Accession: EBI-3647790

Name: EBI-3647774

Confidences:

Type
Value
No records found.

14
EBI-642580
 logo
dastyLogo
PAK-interacting exchange factor beta
Beta-Pix
p85SPR

[+3]
Rho guanine nucleotide exchange factor 7
Mus musculus
Organism Details

Accession: EBI-1329

Name: mouse

Description: Mus musculus

Cross References:

Database
Identifier
Secondary identifier
Qualifier
mouse
-

Expressed In Details

Accession:

Name:

-
prey
Experimental role: prey
Accession: EBI-58

Name: prey

Description: prey

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0498
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Molecule experimentally identified as being captured by a given bait.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference
so
SO:0000358
-
see-also


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Rho guanine nucleotide exchange factor 7

Participant: Rho guanine nucleotide exchange factor 7

Accession: EBI-3647791

Name: EBI-642580

P
Participant: Rho guanine nucleotide exchange factor 7

Participant: Rho guanine nucleotide exchange factor 7

Accession: EBI-3647791

Name: EBI-642580

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Rho guanine nucleotide exchange factor 7

Participant: Rho guanine nucleotide exchange factor 7

Accession: EBI-3647791

Name: EBI-642580

Stoichiometry: 0.0

F
Participant: Rho guanine nucleotide exchange factor 7

Participant: Rho guanine nucleotide exchange factor 7

Accession: EBI-3647791

Name: EBI-642580

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
No records found.

C
Participant: Rho guanine nucleotide exchange factor 7

Participant: Rho guanine nucleotide exchange factor 7

Accession: EBI-3647791

Name: EBI-642580

Confidences:

Type
Value
No records found.