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What is this view?

Binary interactions

In this tab, we display the list of interactions that you have selected using one of our search features. Despite the fact that our data are annotated to accurately reflect the interactions reported in scientific literature, the data is shown in this view as binary interactions. Whenever the data was reported as a co-complex involving more than two molecules, we store it as such in the IntAct database and post-process it so the portal can show it as binary interaction. This post-processing is the Spoke Expansion model (connects bait to all preys):



sourceExp

At any moment you can choose to display the expansion column in this view in order to see which interaction are spoke expanded and which are not.

Description of what has changed

  • We have added more download options to allow users to retrieve their interaction set using more standard formats such as PSI-MI XML and PSIMITAB (version 2.5, 2.6 or 2.7) but also XGMML, RDF and Biopax (level 2 and 3).
  • We have now four different table views : minimal(molecule names and interaction AC), basic (minimal + molecule links, interaction detection method, negative), standard(minimal + molecule species, confidences, publication details, experiment details), expanded (standard + more experiment details) and complete (all mitab 2.7 columns).

Configuring the view to your need"

In the header of the interaction table you will find a button: ‘Change Column Display’ that will show you all the columns/Table views available and allow you to update the current selected set.

Downloading the data into Standard formats"

In the header of the interaction table you will find a drop down list that contains all the formats currently supported when downloading the interaction data. Select one of them and click the export button next to the list. Please note that PSI-MI XML is only available when the interaction set is no bigger than 1000 interactions.

Opening the interaction details"

Clicking on the magnifying glass in the first column of the interaction table will open the details of the corresponding interaction in the Interaction Details tab, giving you access to more details of the manually curated record.

What is this view?

Browsing (Browse Tab)

This tab is meant to give you access to more content based on the currently selected set of interactions. Please note that linking to third party resources will only include up to 200 molecules , if you exceed this number you will see the warning icon (This number has been reduced to 125 molecules for mRNA expression). Now let’s look at the features available to you:

Limiting the scope of the current dataset with the Uniprot Taxonomy ontology

Allows users to browse the Uniprot Taxonomy hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the GO ontology

Allows users to browse the GO hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Limiting the scope of the current dataset with the ChEBI ontology

Allows users to browse the ChEBI hierarchy as a tree and select terms in order to narrow down their dataset. Once a term is selected, you are taken back to the interaction tab to review your dataset.

Bulk linking to third party resources by using involved proteins

  • Proteins by Reactome pathway: Sends your proteins to the Reactome SkyPainter that will show you the pathways in which these molecules are know to play a role.
  • Proteins by Chromosomal location: Sends your list of proteins to Ensembl’s Karyotype viewer and overlays the proteins on the chromosomes.
  • Proteins by mRNA expression: Sends your set of proteins to the ArrayExpress Atlas that will show the known gene expression based on experimental studies.
What is this view?

Searching Interactions (Search Tab)

As you can see in this tab we are now trying to give you more targeted choice to do your queries, please note that the examples provided in this tab are live links so you can simply click them to see the resulting interactions sets.

Using the Quick Search

In this search panel you are free to type anything that might relate to interactions, whether it is properties of their interactor (gene name, identifiers, GO term…) or more specific to the interaction like publication, authors, experimental detection method, ...

Some examples:

  • Try the query: imatinib
    This is a drug for which we have curated a number of interactions.
    Once you press the search button you should be taken to the Interaction Tab that lists 130 binary interactions.
    If you want to construct more complex queries we recommend you take a look at the Molecular Interaction Query Language, accessible from the quick search panel.
  • Try the query: species:yeast AND type:"direct interaction"
    This query selects all interactions involving yeast interactors that have been shown to have direct interactions. If you customize the column display of the interaction tab, you will see that not only “direct interaction” have been selected but also children terms in the PSI-MI ontology.

Using the Ontology Search

Open the Rearch Tab. This panel is specialised to give you an easy access to ontology search. So far you can search on 4 ontologies:

  • Gene Ontology
  • InterPro
  • PSI-MI
  • ChEBI

Whenever you start typing a query in this search panel, the system will search as you type and propose a list of matching controlled vocabulary terms. You can then select one of them and select matching interactions.

For example, type: cancer
You will be presented with a few choices, please note that each term is followed by the count of matching interactions in the IntAct database.

Select a term with the mouse or using the keyboard cursor keys and you will be taken to the interaction tab.

Searching the Compound chemical structure

In this panel you will be able to draw all or part of a chemical structure and search for chemical compounds. If you get any matched, you can then see all interactions involving them.

First you have to open up the chemical search panel so that the applet can load, it might take a few seconds. Then you can start drawing your structure, for instance:

Once you have drawn your structure, select Similarity and press Search. You should be presented with a list of matching compound. Now choose one molecule and click the link: IntAct interactions. You will be taken to the interaction tab to review the data.

Complex Expansion

Binary interactions generated by co-complex expansion

Why should you care about complex expansion ?

Some experimental methods such as Tandem Affinity Purification do generate molecular interactions that can involve more than 2 molecules. Despite the fact that IntAct curation team do capture the molecular interaction as they were reported in the corresponding experiment, when you search using the intact web site, the results of your query is always shown as set of binary interactions (i.e. 2 molecules). We would like to draw your attention on the fact that whenever the reported interaction was a co-complex we do apply an expansion algorithm that transform this n-ary interaction into a set of binary interactions. While none of these agorithms is perfect and will very likely generate some false positive interactions, it is useful to present the data in a consistent manner. Bear in mind that we will strive to differentiate in the search results which interactions are a real experimental binary from expanded ones.

Existing expansion algorithm

There are several known algorithm allowing to transform an n-ary interaction into a set of binaries. The illustration below present the two well known expansion model and illustrates why they can be incorrect.



sourceExp

  • Spoke expansion: Links the bait molecule to all prey molecules. If N is the count of molecule in the complex, it generated N-1 binary interactions.
  • Matrix expansion: Links all molecule to all other molecule present in the complex. If N is the count of molecule in the complex, it generated (N*(N-1))/2 binary interactions.

Now the issue (as illustrated at the bottom right of the diagram above) with these two models lies in the fact that the real complex might not be articulated around the experimental bait but instead, this bait might be linked to a smaller complex, hence most binary interaction generated by spoke and matrix expansion result in false positive.



PSICQUIC

How is the number of interactions in other databases obtained?

PSICQUIC is a standard way to access molecular interaction databases across which it repeats the same query. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the PSICQUIC View, where you can browse the results in those other resources.

The services currently active are:

Check the PSICQUIC site for more information.

IMEx

What is the significance of the IMEx dataset?"

IMEx is a network of databases which have agreed to supply a non-redundant set of data expertly manually annotated to the same consistent detailed standard which, as such, represents a high-quality subset of the data each individually provides. The number of databases providing data may vary, depending on the status of their services and only those that are active are used in this query. By clicking on the number of interactions you will be redirected to the IMEx View, where you can browse the results in those other resources.

The services currently active are:

Check the IMEx site for more information.

What is this view?

Representation of Experimental Features

This section shows the graphical representation of experimental features, where each participant is represented as a white rectangle with a black border and a line for each hundredth amino acid. All available features are attached to their associated participant and their categories are represented in the right side of the legend. The left side of the legend dynamically shows the range statuses occuring in the shown interaction. These are the possible range statuses:

sourceExp

Interacting with the widget

Hover over a feature to see more information in a tooltip.
sourceExp

To display a single interacting region click on it and click again to display all interacting regions.
Displaying all interacting regionsDisplaying one interacting region
sourceExpsourceExp
What is this view?

Dynamic molecular interaction data

This section shows the graphical representation of dynamic molecular interactions. By default it displays all the interactions from one experiment using radio buttons to allow users to highlight interactions in different variable conditions.

Publication

PubMed Id: 16606699

Journal: Genome Res. (1088-9051)

Year of Publication: 2006

Title: Large-scale identification of protein-protein interaction of Escherichia coli K-12.

Author List: Arifuzzaman M., Maeda M., Itoh A., Nishikata K., Takita C., Saito R., Ara T., Nakahigashi K., Huang HC., Hirai A., Tsuzuki K., Nakamura S., Altaf-Ul-Amin M., Oshima T., Baba T., Yamamoto N., Kawamura T., Ioka-Nakamichi T., Kitagawa M., Tomita M., Kanaya S., Wada C., Mori H.

Cross References:

Database
Identifier
Secondary identifier
Qualifier
-

Experiment (1952 interactions)

Accession: EBI-1112582

Name: arifuzzaman-2006-1

Host organism: Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911)

Organism: Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911)

Accession: EBI-852879

Name: ecow3

Description: Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911)

Cross References:

Database
Identifier
Secondary identifier
Qualifier
strain k12 / w3110 / atcc 2732

Interaction Detection Method: pull down

Interaction detection method: pull down
Accession: EBI-1223

Name: pull down

Description: pull down

Cross References:
Database
Identifier
Secondary identifier
Qualifier
pubmed
14755292
-
primary-reference
psi-mi
MI:0096
-
identity


Annotations:
Topic
Text
definition
A specific affinity chromatography method where a molecule of interest (bait) is bound to a column, often via an affinity tag expressed as a protein fusion (GST, HIS tag and others) or chemically linked to the bait molecule . The molecule may be expressed or synthesised and purified first, often in an heterologous system, bound to the matrix at high concentration and then challenged with a solution or cellular extract containing the candidate partner molecules. Alternatively, a multi-molecular complex may be adsorbed to the resin and the retained binding molecules subsequently identified.


Participant Identification Method: ms participant

Participant identification method: ms participant
Accession: EBI-608794

Name: ms participant

Description: Identification by mass spectrometry

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0427
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
identification by mass spectrometry.


Cross References:

Database
Identifier
Secondary identifier
Qualifier
-

Annotations:

Topic
Text
hmori@gtc.naist.jp
Protein identifiers provided in the paper were mapped to UniProtKB accession numbers using an index of GenoBase identifiers to UniProtKB accession numbers. The difference in baits presented in the paper and those presented in the entry is due to the absence of a GenoBase protein identifier to UniProtKB accession number mapping in the index used.
Cell extracts were loaded onto a Nickel (Ni2+) columand equilibrated with buffer I (50 mM Sodium phosphate (pH 7.0), 200 mM NaCl, Proteinase inhibitor), washed three times with 1 mL of buffer 2 (20 mM imidazole and 0.05% n-octyl-beta-glucoside), and proteins were eluted with buffer 3 (50 mM Tris-HCl (pH 6.8), 2% SDS, 0.1% bromophenol blue, 10% glycerol, 100 mM DTT, 6 M Urea and 250mM imidazole). Eluted proteins were analyzed by 7.5 to 15% gradient SDS-PAGE followed by Coomassie Brilliant Blue (CBB-R250) staining. Separated proteins were excised from the gel and identified by standard mass spectrometry techniques.

Interaction

Accession: EBI-1141261

Name: yecj-atpd

Description: -

Type: association

Interaction type: association
Accession: EBI-1813191

Name: association

Description: association

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0914
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Interaction between molecules that may participate in formation of one, but possibly more, physical complexes. Often describes a set of molecules that are co-purified in a single pull-down or coimmunoprecipitation but might participate in formation of distinct physical complexes sharing a common bait.


Participants (12)

Legend:
A
Annotation and Cross Reference  
P
Experimental Parameter   
S
Stoichiometry   
F
Experimental Feature
C
Participant Confidence
#
Name
Links
Primary Identifier
Aliases
Description
Species
Expression system
Experimental role
Biological role
Interactor type
More...
1
EBI-1126091
 logo
yecJ
b4537
JW1891
Uncharacterized protein YecJ
Escherichia coli (strain K12)
Organism Details

Accession: EBI-1395259

Name: ecoli

Description: Escherichia coli (strain K12)

Cross References:

Database
Identifier
Secondary identifier
Qualifier
strain k12
-

Expressed In Details

Accession:

Name:

-
bait
Experimental role: bait
Accession: EBI-49

Name: bait

Description: bait

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0496
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
No records found.


unspecified role
Biological role: unspecified role
Accession: EBI-77781

Name: unspecified role

Description: unspecified role

Cross References:
Database
Identifier
Secondary identifier
Qualifier
psi-mi
MI:0499
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
Role not specified or not applicable to the data.


protein
Interactor type: protein
Accession: EBI-619654

Name: protein

Description: protein

Cross References:
Database
Identifier
Secondary identifier
Qualifier
so
SO:0000358
-
see-also
psi-mi
MI:0326
-
identity
pubmed
14755292
-
primary-reference


Annotations:
Topic
Text
definition
A linear polymer of amino acids joined by peptide bonds in a specific sequence.


A
Participant: Uncharacterized protein YecJ

Participant: Uncharacterized protein YecJ

Accession: EBI-1141262

Name: EBI-1126091

P
Participant: Uncharacterized protein YecJ

Participant: Uncharacterized protein YecJ

Accession: EBI-1141262

Name: EBI-1126091

Parameters:

Type
Value
Unit
Base
Exponent
Uncertainty
No records found.


S
Participant: Uncharacterized protein YecJ

Participant: Uncharacterized protein YecJ

Accession: EBI-1141262

Name: EBI-1126091

Stoichiometry: 1.0

F
Participant: Uncharacterized protein YecJ

Participant: Uncharacterized protein YecJ

Accession: EBI-1141262

Name: EBI-1126091

Features:

Legend:
A
Annotation and Cross Reference  
Feature Name
Feature Type
Detection Method
Range positions
More...
region
  • ?-?
  • A
    Feature: EBI-1156604

    Feature: region

    Accession: EBI-1156604

    Name: region


    C
    Participant: Uncharacterized protein YecJ

    Participant: Uncharacterized protein YecJ

    Accession: EBI-1141262

    Name: EBI-1126091

    Confidences:

    Type
    Value
    No records found.

    2
    EBI-1113934
     logo
    ykgF
    b0307
    JW0300
    Uncharacterized electron transport protein YkgF
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Uncharacterized electron transport protein YkgF

    Participant: Uncharacterized electron transport protein YkgF

    Accession: EBI-1141263

    Name: EBI-1113934

    P
    Participant: Uncharacterized electron transport protein YkgF

    Participant: Uncharacterized electron transport protein YkgF

    Accession: EBI-1141263

    Name: EBI-1113934

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Uncharacterized electron transport protein YkgF

    Participant: Uncharacterized electron transport protein YkgF

    Accession: EBI-1141263

    Name: EBI-1113934

    Stoichiometry: 1.0

    F
    Participant: Uncharacterized electron transport protein YkgF

    Participant: Uncharacterized electron transport protein YkgF

    Accession: EBI-1141263

    Name: EBI-1113934

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Uncharacterized electron transport protein YkgF

    Participant: Uncharacterized electron transport protein YkgF

    Accession: EBI-1141263

    Name: EBI-1113934

    Confidences:

    Type
    Value
    No records found.

    3
    EBI-368783
     logo
    atpD
    papB
    uncD

    [+4]
    ATP synthase subunit beta
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: ATP synthase subunit beta

    Participant: ATP synthase subunit beta

    Accession: EBI-1141264

    Name: EBI-368783

    P
    Participant: ATP synthase subunit beta

    Participant: ATP synthase subunit beta

    Accession: EBI-1141264

    Name: EBI-368783

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: ATP synthase subunit beta

    Participant: ATP synthase subunit beta

    Accession: EBI-1141264

    Name: EBI-368783

    Stoichiometry: 1.0

    F
    Participant: ATP synthase subunit beta

    Participant: ATP synthase subunit beta

    Accession: EBI-1141264

    Name: EBI-368783

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: ATP synthase subunit beta

    Participant: ATP synthase subunit beta

    Accession: EBI-1141264

    Name: EBI-368783

    Confidences:

    Type
    Value
    No records found.

    4
    EBI-1115771
     logo
    yahG
    b0321
    JW0313
    Uncharacterized protein YahG
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Uncharacterized protein YahG

    Participant: Uncharacterized protein YahG

    Accession: EBI-1141265

    Name: EBI-1115771

    P
    Participant: Uncharacterized protein YahG

    Participant: Uncharacterized protein YahG

    Accession: EBI-1141265

    Name: EBI-1115771

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Uncharacterized protein YahG

    Participant: Uncharacterized protein YahG

    Accession: EBI-1141265

    Name: EBI-1115771

    Stoichiometry: 1.0

    F
    Participant: Uncharacterized protein YahG

    Participant: Uncharacterized protein YahG

    Accession: EBI-1141265

    Name: EBI-1115771

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Uncharacterized protein YahG

    Participant: Uncharacterized protein YahG

    Accession: EBI-1141265

    Name: EBI-1115771

    Confidences:

    Type
    Value
    No records found.

    5
    EBI-548960
     logo
    nadE
    efg
    ntrL

    [+4]
    NH(3)-dependent NAD(+) synthetase
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: NH(3)-dependent NAD(+) synthetase

    Participant: NH(3)-dependent NAD(+) synthetase

    Accession: EBI-1141266

    Name: EBI-548960

    P
    Participant: NH(3)-dependent NAD(+) synthetase

    Participant: NH(3)-dependent NAD(+) synthetase

    Accession: EBI-1141266

    Name: EBI-548960

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: NH(3)-dependent NAD(+) synthetase

    Participant: NH(3)-dependent NAD(+) synthetase

    Accession: EBI-1141266

    Name: EBI-548960

    Stoichiometry: 1.0

    F
    Participant: NH(3)-dependent NAD(+) synthetase

    Participant: NH(3)-dependent NAD(+) synthetase

    Accession: EBI-1141266

    Name: EBI-548960

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: NH(3)-dependent NAD(+) synthetase

    Participant: NH(3)-dependent NAD(+) synthetase

    Accession: EBI-1141266

    Name: EBI-548960

    Confidences:

    Type
    Value
    No records found.

    6
    EBI-547573
     logo
    cspB
    b1557
    JW1549
    Cold shock-like protein CspB
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Cold shock-like protein CspB

    Participant: Cold shock-like protein CspB

    Accession: EBI-1141267

    Name: EBI-547573

    P
    Participant: Cold shock-like protein CspB

    Participant: Cold shock-like protein CspB

    Accession: EBI-1141267

    Name: EBI-547573

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Cold shock-like protein CspB

    Participant: Cold shock-like protein CspB

    Accession: EBI-1141267

    Name: EBI-547573

    Stoichiometry: 1.0

    F
    Participant: Cold shock-like protein CspB

    Participant: Cold shock-like protein CspB

    Accession: EBI-1141267

    Name: EBI-547573

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Cold shock-like protein CspB

    Participant: Cold shock-like protein CspB

    Accession: EBI-1141267

    Name: EBI-547573

    Confidences:

    Type
    Value
    No records found.

    7
    EBI-1126099
     logo
    ybbV
    b0510
    JW0498
    Uncharacterized protein YbbV
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Uncharacterized protein YbbV

    Participant: Uncharacterized protein YbbV

    Accession: EBI-1141268

    Name: EBI-1126099

    P
    Participant: Uncharacterized protein YbbV

    Participant: Uncharacterized protein YbbV

    Accession: EBI-1141268

    Name: EBI-1126099

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Uncharacterized protein YbbV

    Participant: Uncharacterized protein YbbV

    Accession: EBI-1141268

    Name: EBI-1126099

    Stoichiometry: 1.0

    F
    Participant: Uncharacterized protein YbbV

    Participant: Uncharacterized protein YbbV

    Accession: EBI-1141268

    Name: EBI-1126099

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Uncharacterized protein YbbV

    Participant: Uncharacterized protein YbbV

    Accession: EBI-1141268

    Name: EBI-1126099

    Confidences:

    Type
    Value
    No records found.

    8
    EBI-549949
     logo
    gltX
    Glutamyl-tRNA synthetase
    JW2395

    [+1]
    Glutamate--tRNA ligase
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Glutamate--tRNA ligase

    Participant: Glutamate--tRNA ligase

    Accession: EBI-1141269

    Name: EBI-549949

    P
    Participant: Glutamate--tRNA ligase

    Participant: Glutamate--tRNA ligase

    Accession: EBI-1141269

    Name: EBI-549949

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Glutamate--tRNA ligase

    Participant: Glutamate--tRNA ligase

    Accession: EBI-1141269

    Name: EBI-549949

    Stoichiometry: 1.0

    F
    Participant: Glutamate--tRNA ligase

    Participant: Glutamate--tRNA ligase

    Accession: EBI-1141269

    Name: EBI-549949

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Glutamate--tRNA ligase

    Participant: Glutamate--tRNA ligase

    Accession: EBI-1141269

    Name: EBI-549949

    Confidences:

    Type
    Value
    No records found.

    9
    EBI-1118363
     logo
    fliI
    fla AIII
    flaC

    [+2]
    Flagellum-specific ATP synthase
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Flagellum-specific ATP synthase

    Participant: Flagellum-specific ATP synthase

    Accession: EBI-1141270

    Name: EBI-1118363

    P
    Participant: Flagellum-specific ATP synthase

    Participant: Flagellum-specific ATP synthase

    Accession: EBI-1141270

    Name: EBI-1118363

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Flagellum-specific ATP synthase

    Participant: Flagellum-specific ATP synthase

    Accession: EBI-1141270

    Name: EBI-1118363

    Stoichiometry: 1.0

    F
    Participant: Flagellum-specific ATP synthase

    Participant: Flagellum-specific ATP synthase

    Accession: EBI-1141270

    Name: EBI-1118363

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Flagellum-specific ATP synthase

    Participant: Flagellum-specific ATP synthase

    Accession: EBI-1141270

    Name: EBI-1118363

    Confidences:

    Type
    Value
    No records found.

    10
    EBI-562720
     logo
    ybfO
    b0703
    JW0692
    Putative uncharacterized protein YbfO
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Putative uncharacterized protein YbfO

    Participant: Putative uncharacterized protein YbfO

    Accession: EBI-1141271

    Name: EBI-562720

    P
    Participant: Putative uncharacterized protein YbfO

    Participant: Putative uncharacterized protein YbfO

    Accession: EBI-1141271

    Name: EBI-562720

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Putative uncharacterized protein YbfO

    Participant: Putative uncharacterized protein YbfO

    Accession: EBI-1141271

    Name: EBI-562720

    Stoichiometry: 1.0

    F
    Participant: Putative uncharacterized protein YbfO

    Participant: Putative uncharacterized protein YbfO

    Accession: EBI-1141271

    Name: EBI-562720

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Putative uncharacterized protein YbfO

    Participant: Putative uncharacterized protein YbfO

    Accession: EBI-1141271

    Name: EBI-562720

    Confidences:

    Type
    Value
    No records found.

    11
    EBI-542419
     logo
    pepD
    pepH
    b0237

    [+7]
    Cytosol non-specific dipeptidase
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Cytosol non-specific dipeptidase

    Participant: Cytosol non-specific dipeptidase

    Accession: EBI-1141272

    Name: EBI-542419

    P
    Participant: Cytosol non-specific dipeptidase

    Participant: Cytosol non-specific dipeptidase

    Accession: EBI-1141272

    Name: EBI-542419

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Cytosol non-specific dipeptidase

    Participant: Cytosol non-specific dipeptidase

    Accession: EBI-1141272

    Name: EBI-542419

    Stoichiometry: 1.0

    F
    Participant: Cytosol non-specific dipeptidase

    Participant: Cytosol non-specific dipeptidase

    Accession: EBI-1141272

    Name: EBI-542419

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Cytosol non-specific dipeptidase

    Participant: Cytosol non-specific dipeptidase

    Accession: EBI-1141272

    Name: EBI-542419

    Confidences:

    Type
    Value
    No records found.

    12
    EBI-1126106
     logo
    yjbL
    b4047
    JW4007
    Uncharacterized protein YjbL
    Escherichia coli (strain K12)
    Organism Details

    Accession: EBI-1395259

    Name: ecoli

    Description: Escherichia coli (strain K12)

    Cross References:

    Database
    Identifier
    Secondary identifier
    Qualifier
    strain k12
    -

    Expressed In Details

    Accession:

    Name:

    -
    prey
    Experimental role: prey
    Accession: EBI-58

    Name: prey

    Description: prey

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0498
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Molecule experimentally identified as being captured by a given bait.


    unspecified role
    Biological role: unspecified role
    Accession: EBI-77781

    Name: unspecified role

    Description: unspecified role

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    psi-mi
    MI:0499
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    Role not specified or not applicable to the data.


    protein
    Interactor type: protein
    Accession: EBI-619654

    Name: protein

    Description: protein

    Cross References:
    Database
    Identifier
    Secondary identifier
    Qualifier
    so
    SO:0000358
    -
    see-also
    psi-mi
    MI:0326
    -
    identity
    pubmed
    14755292
    -
    primary-reference


    Annotations:
    Topic
    Text
    definition
    A linear polymer of amino acids joined by peptide bonds in a specific sequence.


    A
    Participant: Uncharacterized protein YjbL

    Participant: Uncharacterized protein YjbL

    Accession: EBI-1141273

    Name: EBI-1126106

    P
    Participant: Uncharacterized protein YjbL

    Participant: Uncharacterized protein YjbL

    Accession: EBI-1141273

    Name: EBI-1126106

    Parameters:

    Type
    Value
    Unit
    Base
    Exponent
    Uncertainty
    No records found.


    S
    Participant: Uncharacterized protein YjbL

    Participant: Uncharacterized protein YjbL

    Accession: EBI-1141273

    Name: EBI-1126106

    Stoichiometry: 1.0

    F
    Participant: Uncharacterized protein YjbL

    Participant: Uncharacterized protein YjbL

    Accession: EBI-1141273

    Name: EBI-1126106

    Features:

    Legend:
    A
    Annotation and Cross Reference  
    Feature Name
    Feature Type
    Detection Method
    Range positions
    More...
    No records found.

    C
    Participant: Uncharacterized protein YjbL

    Participant: Uncharacterized protein YjbL

    Accession: EBI-1141273

    Name: EBI-1126106

    Confidences:

    Type
    Value
    No records found.


    Graphical Representation of Experimental Features