DNA polymerase III, beta sliding clamp processivity factor complex

Species: Escherichia coli (strain K12); 83333
Accession number: EBI-6468862

Summary


Systematic Name:

dnaN dimer

Synonyms:
beta sliding clamp processivity factor processivity factor
beta processivity factor sliding beta-clamp
sliding clamp beta clamp
Bioactive entity
Protein
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Gene
DNA
RNA


Function:

Homodimer

Forms a homodimer that is loaded onto DNA at the replication fork by the clamp loader complex (EBI-6467993) and tethers the DNA polymerase III (EBI-6467979) to DNA. At least 3 beta homodimers seem to be present at each replications folk; two are tethered to the two active polymerase core subcomplexes while a third appears to be a little further away, possibly still attached to the end of an Okasaki fragment prior to primer removal and ligation of the gap.

Properties:

One beta homodimer interacts with the alpha subunit of each DNA polymerase III core subcomplex (EBI-6467979) and with the delta subunit of the clamp loader complex (EBI-6467993).


Participants

ID Name Description Stoichiometry Biological Role Interactor Type Other Features
P0A988
dnaN DNA polymerase III subunit beta 2 enzyme protein
binding region P0A988 [1-366]
binding region P0A988 [1-125] [272-273]
binding region P0A988 [242-242] [36-36] [362-362] [274-278] [177-177]

Cross References

Type Database Identifier Description
biological process gene ontology GO:0006271 DNA strand elongation involved in DNA replication
molecular function gene ontology GO:0003677 DNA binding
cellular component gene ontology GO:0044775 DNA polymerase III, beta sliding clamp processivity factor complex
evidence ontology ECO:0000353
molecular function gene ontology GO:0030337 DNA polymerase processivity factor activity
Experimental evidence that interaction occurs intact EBI-1031818
Experimental evidence that interaction occurs intact EBI-6421487
additional information pubmed 20413500
identical object in an external resource wwpdb 2pol
identical object in an external resource wwpdb 1jql
identical object in an external resource wwpdb 1jqj
identical object in an external resource intenz 2.7.7.7
Curated by: IntAct