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Ribonucleotide-diphosphate reductase variant 1

Species: Saccharomyces cerevisiae; 559292
Accession number: EBI-1396664

Summary


Systematic Name:

2xRNR1:RNR2:RNR4

Synonyms:
Ribonucleotide-diphosphate reductase Ribonucleotide reductase
RNR 2'-deoxyribonucleoside-diphosphate:oxidized-thioredoxin 2'-oxidoreductase
Ribonucleoside 5'-diphosphate reductase Ribonucleoside diphosphate reductase
Ribonucleotide diphosphate reductase
Bioactive entity
Protein
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Gene
DNA
RNA


Function:

Heterotetramer

Catalyzes the reduction of ribonucleotides to the corresponding deoxyribonucleotides, an essential step in the de novo synthesis of monomeric precursors for DNA replication and repair. The catalytically active form is an alpha2beta2 tetramer. The homodimeric alpha subunit, called R1, houses the active site, composed of redox-active disulfides, and binding sites for allosteric effectors, ribonucleoside diphosphates. The beta subunit, called R2, contains a di-iron cluster that in its reduced state reacts with dioxygen to form a stable tyrosyl radical (Y*) and a di-iron(III) cluster. This essential Y* is proposed to generate a thiyl radical, located on a cysteine residue in the R1 active site, that initiates ribonucleotide reduction. The enzyme is allosterically controlled by relative levels of dNTPs. This variant is found in all cell states, a related variant (EBI-1396793) is only found in damaged or growth-arrested cells.

Properties:

Sedimention coefficient = 9.7 S


Participants

ID Name Description Stoichiometry Biological Role Interactor Type Other Features
P21524
RNR1 Ribonucleoside-diphosphate reductase large chain 1 2 unspecified role protein
binding region P21524 [1-888]
P09938
RNR2 Ribonucleoside-diphosphate reductase small chain 1 1 unspecified role protein
P49723
RNR4 Ribonucleoside-diphosphate reductase small chain 2 1 unspecified role protein

Cross References

Type Database Identifier Description
Experimental evidence that interaction occurs intact EBI-1031866
additional information pubmed 16537480
additional information pubmed 16537479
molecular function gene ontology GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
biological process gene ontology GO:0009262 deoxyribonucleotide metabolic process
cellular component gene ontology GO:0005971 ribonucleoside-diphosphate reductase complex
molecular function gene ontology GO:0005524 ATP binding
Experimental evidence that interaction occurs intact EBI-1396608
evidence ontology ECO:0000353
identical object in an external resource intenz 1.17.4.1
identical object in an external resource wwpdb 1JK0
Curated by: IntAct