ESCRT I complex

Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 559292
Accession number: EBI-1268517

Summary


Systematic Name:

MVB12:SRN2:STP22:VPS28

Synonyms:
ESCRT-I complex ESCRT1 complex Endosomal sorting complex required for transport-I complex.
Function:

The ESCRT machinery is required for the downregulation of cell-surface receptors and for the final membrane scission step during endocytosis. The ESCRT-I protein complex directs the lysosomal degradation of ubiquinated transmembrane proteins. Consists of a headpiece attached to a stalk, the latter being important for the stability and assembly of the complex, and may act as a spacer regulating the disposition of the cargo. Is recruited to the multivesicular body (MVB) vesicles by ESCRT-0 (EBI-2307938) which localizes to the endosome by binding to the lipid phosphatidylinositol 3-phosphate. On the endosomal membrane both ESCRT-I and ESCRT-0 bind to ubiquitinated endosomal cargo. A docked model places the stalk parallel to the plane of the membrane, but not in direct contact with it, spatially organizing interactions of membrane-bound structures by imposing physical restrictions on their distances relative to each other.

Properties:

MW = 350kDa. The core complex is 18nM long, the stalk being 13nM.

Complex Assembly:

Heterotetramer


Participants

ID Name Description Stochiometry Biological Role Interactor Type Linked Features Other Features
P25604
EBI-411625
STP22 Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease 1 unspecified role protein
P42939
EBI-23478
MVB12 Multivesicular body sorting factor 12 1 unspecified role protein
Q02767
EBI-20387
VPS28 Vacuolar protein sorting-associated protein 28 1 unspecified role protein
Q99176
EBI-18076
SRN2 Protein SRN2 1 unspecified role protein

Cross References

Type Database Identifier Description
evidence ontology ECO:0000353
biological process gene ontology GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular component gene ontology GO:0000813 ESCRT I complex
Experimental evidence that interaction occurs intact EBI-1267663
Experimental evidence that interaction occurs intact EBI-795587
additional information pubmed 17135292
additional information pubmed 17145965
additional information pubmed 17442384
identical object in an external resource wwpdb 2p22