ESCRT I complex
Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 559292
Accession number: EBI-1268517
|ESCRT-I complex||ESCRT1 complex||Endosomal sorting complex required for transport-I complex.|
The ESCRT machinery is required for the downregulation of cell-surface receptors and for the final membrane scission step during endocytosis. The ESCRT-I protein complex directs the lysosomal degradation of ubiquinated transmembrane proteins. Consists of a headpiece attached to a stalk, the latter being important for the stability and assembly of the complex, and may act as a spacer regulating the disposition of the cargo. Is recruited to the multivesicular body (MVB) vesicles by ESCRT-0 (EBI-2307938) which localizes to the endosome by binding to the lipid phosphatidylinositol 3-phosphate. On the endosomal membrane both ESCRT-I and ESCRT-0 bind to ubiquitinated endosomal cargo. A docked model places the stalk parallel to the plane of the membrane, but not in direct contact with it, spatially organizing interactions of membrane-bound structures by imposing physical restrictions on their distances relative to each other.
MW = 350kDa. The core complex is 18nM long, the stalk being 13nM.
|ID||Name||Description||Stochiometry||Biological Role||Interactor Type||Linked Features||Other Features|
|STP22||Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease||1||unspecified role||protein|
|MVB12||Multivesicular body sorting factor 12||1||unspecified role||protein|
|VPS28||Vacuolar protein sorting-associated protein 28||1||unspecified role||protein|
|SRN2||Protein SRN2||1||unspecified role||protein|
|biological process||gene ontology||GO:0043162||ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|
|cellular component||gene ontology||GO:0000813||ESCRT I complex|
|Experimental evidence that interaction occurs||intact||EBI-1267663|
|Experimental evidence that interaction occurs||intact||EBI-795587|
|identical object in an external resource||wwpdb||2p22|