elongator holoenzyme complex

Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 559292
Accession number: EBI-1254644

Summary


Systematic Name:

IKI3:ELP2:ELP3:ELP4:IKI1:ELP6

Synonyms:
elongator complex RNA polymerase II elongator complex
Function:

Histone acetyltransferase component of the chromatin-associated, hyper-phosphorylated form of RNAPII which acts to acetylate the nucleosome in front of elongation machinery, so promoting RNA polymerase elongation, to polarize exocytosis, and to form modified wobble uridines in tRNA. Binds to both naked and nucleosomal DNA and acetylates histones H3, preferentially at Lys-14, and H4, preferentially at Lys-8. Required for the formation of the mcm5 group of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) of two tRNA species. In cells with normal levels of these tRNAs, the presence of mcm5s2U is crucial for posttranscriptional expression of gene products important in transcription and exocytosis.

Properties:

Composed of two weakly associated subcomplexes - Elp1-Elp2-Elp3 and Elp4-Elp5-Elp6. The Elp3 protein is the catalytic subunit, is crucial for the integrity of the holo-Elongator complex and is essential for RNA binding.


Participants

ID Name Description Stochiometry Biological Role Interactor Type Other Features
Q06706
EBI-1254651
IKI3 Elongator complex protein 1 1 unspecified role protein
P42935
EBI-1254650
ELP2 Elongator complex protein 2 1 unspecified role protein
Q02884
EBI-1254649
ELP4 Elongator complex protein 4 1 unspecified role protein
Q02908
EBI-1254648
ELP3 Elongator complex protein 3 1 enzyme protein
Q04868
EBI-1254647
ELP6 Elongator complex protein 6 1 unspecified role protein
P38874
EBI-1254646
IKI1 Elongator complex protein 5 1 unspecified role protein

Cross References

Type Database Identifier Description
evidence ontology ECO:0000353
biological process gene ontology GO:0002098 tRNA wobble uridine modification
biological process gene ontology GO:0006357 regulation of transcription from RNA polymerase II promoter
cellular component gene ontology GO:0033588 Elongator holoenzyme complex
molecular function gene ontology GO:0003677 DNA binding
molecular function gene ontology GO:0003723 RNA binding
molecular function gene ontology GO:0004402 histone acetyltransferase activity
Experimental evidence that interaction occurs intact EBI-1254421
Experimental evidence that interaction occurs intact EBI-1254447
Experimental evidence that interaction occurs intact EBI-1254570
identical object in an external resource intenz 2.3.1.48
additional information pubmed 12676794
additional information pubmed 15020047
additional information pubmed 17320508