Protein kinase CK2 variant 7

Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 559292
Accession number: EBI-1253728

Summary


Systematic Name:

2xCKA1:2xCKB2

Synonyms:
CK2 CK-2
CKII casein kinase 2
casein kinase II CSK21-CSK2C complex
Bioactive entity
Protein
- click or tap to toggle between circle and bar (bar shows binding sites, if known)
Gene
DNA
RNA


Function:

Heterotetramer

CK2 is composed of a dimer of the regulatory subunit flanked on each side by a catalytic subunit. Phosphorylates serine or threonine residues proximal to acidic amino acids (consensus Ser-Xaa-Xaa-Acidic where acidic residue may be Glu, Asp, pSer or pTYr). Thought to be a dual-specificity kinase in yeast, also able to phosphorylate tyrosine residues, although with less favourable kinetic parameters. ATP or GTP can serve as a phosphate donor. Disruption of CKA1 or CKA2 is not lethal, but disruption of both is synthetic lethal. CKA1 and CKA2 functional overlap is not complete, as yeast with temperature sensitive alleles of CKA1 or CKA2 display distinct phenotypes and different combinations of CKA subunits affect substrate specificity and sub-cellular localization. Disruption of CKB1 or CKB2 causes sensitivity to NaCl and LiCl. Disruption of both CKB1 and CKB2 is not synthetic lethal, although the regulatory subunits are responsible for the structural integrity of the holoenzyme. Much of CK2B is phosphorylated on autophosphorylation site in cells. CK2B is also phosphorylated in a cell cycle-dependent manner. CK2 is involved in regulation of cell cycle progression, presumably through phosphorylation of target proteins. CK2 may also have role(s) in inhibiting apoptosis.

Properties:

CK2 is composed of a dimer of the regulatory subunit flanked on each side by a catalytic subunit. MW = 151,000 Da


Participants

ID Name Description Stoichiometry Biological Role Interactor Type Other Features
P15790
CKA1 Casein kinase II subunit alpha 2 enzyme protein
P38930
CKB2 Casein kinase II subunit beta' 2 unspecified role protein

Cross References

Type Database Identifier Description
additional information pubmed 16607517
molecular function gene ontology GO:0004674 protein serine/threonine kinase activity
Experimental evidence that interaction occurs intact EBI-1249210
Experimental evidence that interaction occurs intact EBI-1249162
Experimental evidence that interaction occurs intact EBI-1249192
cellular component gene ontology GO:0005956 protein kinase CK2 complex
biological process gene ontology GO:0051726 regulation of cell cycle
additional information pubmed 7592778
additional information pubmed 12396231
evidence ontology ECO:0000353
identical object in an external resource intenz 2.7.11.1
Curated by: IntAct