6-phosphofructokinase complex

Species: Schizosaccharomyces pombe (strain ATCC 38366 / 972); 284812
Accession number: EBI-1215423

Summary


Systematic Name:

pfk1 octamer

Synonyms:
6-phosphofructokinase phosphofructokinase
phosphohexokinase 6PF-1-K
Pfk-1
Bioactive entity
Protein
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Gene
DNA
RNA


Function:

Homooctamer

6-phosphofructokinase catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate in the presence of MgATP, the first irreversible step for glycolysis. The enzyme exhibits weak cooperative binding of Fructose 6-P. Its activity is controlled by many allosteric activators and inhibitors (including ATP),showing its crucial role in regulation of glycolytic flux. The effectors either modulate the affinity of Pfk for Fructose 6-P or overcome inhibitory effects. Pfk is an octamer composed of 8 identical subunits.

Properties:

MW = 790 (plus or minus 30) kDa. Each identical subunit is approximately 100 (plus or minus 5) kDa. Apparent sedimentation coefficient, s20,c = 20.2 (plus or minus 0.3) S.


Participants

ID Name Description Stoichiometry Biological Role Interactor Type Other Features
O42938
pfk1 ATP-dependent 6-phosphofructokinase 8 unspecified role protein

Cross References

Type Database Identifier Description
additional information pubmed 12051897
cellular component gene ontology GO:0005945 6-phosphofructokinase complex
biological process gene ontology GO:0006096 glycolysis
molecular function gene ontology GO:0003872 6-phosphofructokinase activity
Experimental evidence that interaction occurs intact EBI-1215419
evidence ontology ECO:0000353
identical object in an external resource intenz 2.7.1.11
Curated by: IntAct