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6-phosphofructokinase complex

Species: Saccharomyces cerevisiae; 559292
Accession number: EBI-1212614

Summary


Systematic Name:

4xPFK1:4xPFK2

Synonyms:
6-phosphofructokinase phosphofructokinase
phosphohexokinase 6PF-1-K complex
Pfk-1 complex PFK complex
Bioactive entity
Protein
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Gene
DNA
RNA


Function:

Heterooctamer

Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate in the presence of MgATP, the first irreversible step for glycolysis. The enzyme is stablilized in the presence of Fructose 6-P and exhibits cooperative binding of Fructose 6-P. Its activity is controlled by many allosteric activators and inhibitors (including ATP), showing its crucial role in regulation of glycolytic flux. The enzyme can exist in either the active R-state or inhibited T-state. The effectors either modulate the affinity of Pfk for Fructose 6-P or overcome inhibitory effects. Pfk is an octamer composed of 4 alpha and 4 beta subunits.

Properties:

MW = 835 kDA (approximately). Each subunit is approximately 105 kDa. The native octameric structure has a sedimentation coefficient of 21 S. The molecule is elongated with length of 24 nm and width of 17 nm. There is mild asymmetry between the top and bottom tetramers, each composed of 2 alpha and 2 beta subunits.


Participants

ID Name Description Stoichiometry Biological Role Interactor Type Other Features
P16861
PFK1 ATP-dependent 6-phosphofructokinase subunit alpha 4 unspecified role protein
binding region P16861 [?-?]
P16862
PFK2 ATP-dependent 6-phosphofructokinase subunit beta 4 unspecified role protein
binding region P16862 [?-?]
binding region P16862 [?-?]

Cross References

Type Database Identifier Description
cellular component gene ontology GO:0005945 6-phosphofructokinase complex
biological process gene ontology GO:0006096 glycolytic process
Experimental evidence that interaction occurs intact EBI-1212520
molecular function gene ontology GO:0003872 6-phosphofructokinase activity
additional information pubmed 11015725
evidence ontology ECO:0000353
identical object in an external resource intenz 2.7.1.11
identical object in an external resource wwpdb 3o8o
Curated by: IntAct