Degradosome

Species: Escherichia coli (strain K12); 83333
Accession number: EBI-1169250

Summary


Systematic Name:

8xrhlB:12xpnp:16xeno:12xrne

Synonyms:
RNA degradosome - core component RNA degradosome
Degradosome
Bioactive entity
Protein
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Gene
DNA
RNA


Function:

Key role in mRNA degradation and RNA processing. RNA binds to RnaE, RhlB helicase is necessary to unwind the RNA secondary structures and, once unwound, the 3?-5? decay of the transcripts is ensued by phosphate-dependent PNPase. RNase E bound-enolase may connect cellular metabolic status with post-transcriptional gene regulation and link RNA degradation to glycolytic processes. A dynamic set of minor components of this complex may regulate its function and compartmentalization.

Properties:

Structured as ordered helical elements arranged in a coil. RNase E provides the backbone of the complex and the N-terminus of this enzyme tethers the degradosome to the cytoplasmic membrane. A ratio of four PNPase trimers binds to three RNase E tetramers and eight enolase dimers and eight RhlB helicase N-terminal domains bind to the C-terminal tails of RNase E. Mg2+ ions coordinate the N-terminal domains of RNase E into catalytic tetrameric domains.


Participants

ID Name Description Stoichiometry Biological Role Interactor Type Other Features
P0A6P9
eno Enolase 16 unspecified role protein
P05055
pnp Polyribonucleotide nucleotidyltransferase 12 unspecified role protein
P21513
rne Ribonuclease E 12 unspecified role protein
P0A8J8
rhlB ATP-dependent RNA helicase RhlB 8 enzyme protein

Cross References

Type Database Identifier Description
evidence ontology ECO:0000353
molecular function gene ontology GO:0003723 RNA binding
molecular function gene ontology GO:0005524 ATP binding
cellular component gene ontology GO:1990061 bacterial degradosome
molecular function gene ontology GO:0000287 magnesium ion binding
additional information pubmed 21805185
molecular function gene ontology GO:0004519 endonuclease activity
cellular component gene ontology GO:0016021 integral component of membrane
Experimental evidence that interaction occurs intact EBI-849044
biological process gene ontology GO:0006401 RNA catabolic process
biological process gene ontology GO:0006396 RNA processing
Curated by: IntAct