degradosome

Species: Escherichia coli (strain K12); 83333
Accession number: EBI-1169250

Summary


Systematic Name:

8xrhlB:12xpnp:16xeno:12xrne

Synonyms:
RNA degradosome - core component RNA degradosome Degradosome
RNA degradosome - core component RNA degradosome
Function:

Key role in mRNA degradation and RNA processing. RNA binds to RnaE, RhlB helicase is necessary to unwind the RNA secondary structures and, once unwound, the 3′-5′ decay of the transcripts is ensued by phosphate-dependent PNPase. RNase E bound-enolase may connect cellular metabolic status with post-transcriptional gene regulation and link RNA degradation to glycolytic processes. A dynamic set of minor components of this complex may regulate its function and compartmentalization.

Properties:

Structured as ordered helical elements arranged in a coil. RNase E provides the backbone of the complex and the N-terminus of this enzyme tethers the degradosome to the cytoplasmic membrane. A ratio of four PNPase trimers binds to three RNase E tetramers and eight enolase dimers and eight RhlB helicase N-terminal domains bind to the C-terminal tails of RNase E. Mg2+ ions coordinate the N-terminal domains of RNase E into catalytic tetrameric domains.


Participants

ID Name Description Stochiometry Biological Role Interactor Type Linked Features Other Features
P21513
EBI-549958
rne Ribonuclease E 12 unspecified role protein
binding-associated region P0A8J8 [628-843]
binding-associated region P05055 [1021-1061]
binding-associated region P0A6P9 [839-850]
P0A6P9
EBI-368855
eno Enolase 16 unspecified role protein
binding-associated region P21513 [2-432]
P05055
EBI-548080
pnp Polyribonucleotide nucleotidyltransferase 12 unspecified role protein
binding-associated region P21513 [1-711]
P0A8J8
EBI-555806
rhlB ATP-dependent RNA helicase RhlB 8 enzyme protein
binding-associated region P21513 [2-421]

Cross References

Type Database Identifier Description
evidence ontology ECO:0000353
biological process gene ontology GO:0006396 RNA processing
biological process gene ontology GO:0006401 RNA catabolic process
cellular component gene ontology GO:1990061 bacterial degradosome
molecular function gene ontology GO:0000287 magnesium ion binding
molecular function gene ontology GO:0003723 RNA binding
molecular function gene ontology GO:0004519 endonuclease activity
molecular function gene ontology GO:0005524 ATP binding
Experimental evidence that interaction occurs intact EBI-849044
additional information pubmed 21805185