Alignments generated with PRANK
and PRANKSTER contain more
information than can be stored using the traditional sequence alignment
formats, so we have defined a new alignment format called HSAML. Both
PRANK and PRANKSTER support saving data in the HSAML format, and PRANKSTER
provides a convenient graphical front-end to open and browse these
The HSAML format follows the XML standards and, in
addition to PRANKSTER, any program supporting the XML is able to open and
manipulate the alignments. Two of the possible software packages to
handle the data are
R (see here), a free
software environment for statistical computing and graphics, and
(see here), a
cross-platform programming language. We will not go into details of
perl here but provide simple examples how to
manipulate HSAML-formatted data using these two packages. Which ever is
your choice of a programming language, it is strongly recommended that
you use one of the existing XML parsers to import the HSAML alignments
and will not try to read them as text files!
schema gives a formal definition of the HSAML format.
An alignment generated by PRANK/PRANKSTER consist of four
elements: newick, nodes, selection
and model; sub-elements
of nodes are either leaf or node.
Colours refer to this example
The element newick
defines the alignment guide tree (or phylogeny) in the 'newick' format.
The tree should be complete and have a name for each node (terminal and
internal) and a branch length for each branch. A node name and branch
length are separated by a colon, two sister nodes separated by a comma
and surrounded by a pair of brackets. A node name can be any
alpha-numeric string (A-Za-z0-9 + some other characters) except that
the names for internal nodes are always surrounded by hash signs (#)
and the names for terminal nodes never contain hashes. It is convenient
to keep the node names short and provide more information in nodes.
The element nodes
contains terminal and internal nodes. A terminal node is defined in leaf and has attributes 'id',
which matches a node name in the newick tree, and 'name',the full name
of the sequence. The only content of a leaf is 'sequence', an aligned
sequence. An internal node is defined in node
and has one attribute, 'id' that matches a node name in the newick
tree. The contents of a node are of the type 'probability', each having
the attribute 'id' matching one of the process id's in the element model.
The element selection
is optional and only appears when a user has deselected some of the
alignment sites using filters based on the sitewise posterior
probabilities. Selection consists of 'selected_sites', a boolean vector
defining if a site is selected/deselected, and 'selection_criteria', a
description of the process resulting to the current selection.
The element model
has two purposes: first, it gives further information of the processes
for which nodes contain posterior probabilities for (attributes 'id'
and 'name'), and, second, it specifies how PRANKSTER displays this
information. The meaning of different attributes becomes obvious if one
plays with PRANKSTER (Settings->Plot), and the range of possible
attribute values is defined in the XML
R script plots the
posterior probability of one of the processes across the alignment
sites. If you download the script here
and the example alignment here,
R and type the command
you should obtain a plot similar to this:
reproduce the plot shown here, you should download this script.)
The plotted value is the posterior probability of the slow
process across the alignment sites in the three internal nodes. In the
two latter plots, the broad peaks match rather well with the known
coding exons that are indicated by the black bars in the top (not
included in the example script).
Note that the script requires
package to be installed. R packages can be found from the project homepage following
the links to
Consult the R manual to learn more about the package installation.
perl script demonstrates
how to select nodes and clades of an alignment tree, manipulate
sequences, extract posterior probability values and work with names and
id's. A detailed explanation of the use of the script and the functions
implemented in the module is given in the source code file.
The script and the additional module required by the
script can be downloaded from here
and here, and the use of
the script tested with this
example dataset. Typing
should read in the alignment and print out the location and sequence of
the sites for which the alignment reliability score is below 50% in the
Note that the script requires the XML::libXML module to
be installed (download it from here).
Consult the perl manual to learn more about the package installation.
The XML schema was defined by Nicolas Rodriguez.
PRANK front page.
Comments? E-mail firstname.lastname@example.org.