ID Z74787; SV 1; linear; genomic DNA; STD; UNC; 8520 BP. XX AC Z74787; XX DT 17-JUL-1996 (Rel. 48, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 14) XX DE Bacterial species plasmid pIE1107 DNA XX KW aminoglycoside-3'-phosphotransferase; aphA gene; sat3 gene; KW streptothricin-acetyltransferase. XX OS IncQ-like plasmid pIE1107 OC other sequences; plasmids. XX RN [1] RP 1-8520 RA Tietze E., Erhard; RT "Nucleotide sequence and genetic characterization of the novel IncQ-like RT Streptothricin Resistance plasmid pIE1107."; RL Unpublished. XX RN [2] RC (sites) RX DOI; 10.1016/0378-1119(89)90273-4. RX PUBMED; 2653965. RA Scholz P., Haring V., Wittmann-Liebold B., Ashman K., Bagdasarian M., RA Scherzinger E.; RT "Complete nucleotide sequence and gene organization of the broad-host-range RT plasmid RSF1010"; RL Gene 75(2):271-288(1989). XX RN [3] RC (sites) RX DOI; 10.1016/0167-4781(95)00103-N. RX PUBMED; 7640311. RA Tietze E., Brevet J.; RT "Nucleotide sequence of the bacterial streptothricin resistance gene sat3"; RL Biochim. Biophys. Acta 1263(2):176-178(1995). XX RN [4] RP 1-8520 RA Tietze E.; RT ; RL Submitted (15-JUL-1996) to the INSDC. RL Tietze E., Robert Koch-Institut, Bereich Wernigerode, Burgstrasse 37, RL Wernigerode, Federal Republic of Germany, D 38855 XX RN [5] RX DOI; 10.1006/plas.1998.1343. RX PUBMED; 9571133. RA Tietze E.; RT "Nucleotide sequence and genetic characterization of the novel IncQ-like RT plasmid pIE1107"; RL Plasmid 39(3):165-181(1998). XX DR RFAM; RF00043; Plasmid_R1162. XX FH Key Location/Qualifiers FH FT source 1..8520 FT /organism="IncQ-like plasmid pIE1107" FT /mol_type="genomic DNA" FT /db_xref="taxon:123385" FT misc_feature 1..3160 FT /note="IncQ-like-DNA" FT /note="This region with 75% average homology to the FT nucleotide sequence of IncQ plasmid RSF1010 (2203-5385) is FT also similar in gene organization (analogs of oriV, oriT, FT mobA, mobB, mobC, repB of RSF1010)." FT /citation=[2] FT /citation=[1] FT misc_feature 3478..5206 FT /note="IncQ-like-DNA" FT /note="This region with 90% average homology to the FT nucleotide sequence of IncQ plasmid RSF1010 (5845-7572) is FT also similar in gene organization (analogs of repA, repC of FT RSF1010)." FT /citation=[2] FT /citation=[1] FT iDNA 3161..3477 FT /note="This region interrupts homology with RSF1010 FT nucleotide sequence. 313 bp of unknown function in pIE1107 FT replace 461 bp of coding DNA of RSF1010." FT /citation=[2] FT /citation=[1] FT iDNA 5310..7596 FT /note="This region interrupts homology with RSF1010. It FT contains two resistance genes not present in RSF1010. A FT perfectly conserved copy of this DNA is present in another FT IncQ plasmid, pIE639." FT /citation=[2] FT /citation=[3] FT /citation=[1] FT misc_feature 7597..8520 FT /note="IncQ-like-DNA" FT /note="This region is 99% homologous with the nucleotide FT sequence of IncQ plasmid RSF1010 (7640-8559). It contains FT part of the sulII gene present in RSF1010." FT /citation=[2] FT /citation=[1] FT rep_origin 145..519 FT /note="This region with 96% homology to the nucleotide FT sequence of the oriV in RSF1010 is dispensable for FT replication of pIE1107." FT /citation=[2] FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 148..211 FT /function="incompatibility determinant" FT /rpt_type=DIRECT FT /rpt_unit_range=148..167 FT /note="These DR are identical to the iterons in the oriV of FT RSF1010. They cause incompatibility with RSF1010 but not FT with pIE1107." FT /citation=[2] FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT rep_origin <5226..>5648 FT /note="This region with 88% homology to the oriV of RSF1010 FT is essential for replication of pIE1107." FT /note="Location changed from '(5202.5226)..(5648.5657)' to FT '<5226..>5648'" FT /citation=[2] FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 5226..5309 FT /function="incompatibility determinant" FT /rpt_type=DIRECT FT /rpt_unit_range=5226..5245 FT /note="These iterons, if cloned on a multicopy vector, FT cause strong incompatibility towards pIE1107 but not FT towards RSF1010." FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT CDS complement(6095..6910) FT /transl_table=11 FT /gene="aphA" FT /standard_name="aph(3')-Id" FT /product="aminoglycoside-3'-phosphotransferase" FT /function="determinant for resistance to kanamycin and FT neomycin" FT /note="This novel aphA gene has about 60% nucleotide FT sequence homology with other aph(3')-I genes. The amino FT acid sequence homology is around 60% with APH(3')-I FT proteins but less 40% with APH(3')-II to VII." FT /db_xref="GOA:Q57314" FT /db_xref="HSSP:1ND4" FT /db_xref="InterPro:IPR002575" FT /db_xref="InterPro:IPR011009" FT /db_xref="InterPro:IPR024165" FT /db_xref="UniProtKB/TrEMBL:Q57314" FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA99049.1" FT /translation="MSEWRRQQVCAPIPMPASMATSLEGYQWAPITIGESGSNVYRLYG FT KPKAPDLFLKRGKYDVADDVTDEMVRLRWLAERIPVPTVVNFVRTLDEAWLLTTAMPGR FT TVYEELEANPDACLAIADALADFLRRLHEIPTIECPFNSNHIYRLALARKRIEAGMVEV FT DDFDDERKGWAAEQVWDAMHNLLPFTPDPVTTHGDSALENLFVREGKVTGCIDVGRSGV FT ADRYQDIAILWNRLGEFGSLPQERFVEQYGIADVDRRKLSFHLMLDELF" FT CDS complement(6967..7509) FT /transl_table=11 FT /gene="sat3" FT /standard_name="streptothricin-acetyltransferase-3 gene" FT /product="streptothricin-acetyltransferase" FT /function="determinant for resistance to streptothricin FT antibiotics" FT /note="This gene is also present in the IncQ plasmid FT pIE639." FT /db_xref="GOA:Q57025" FT /db_xref="InterPro:IPR000182" FT /db_xref="InterPro:IPR008125" FT /db_xref="InterPro:IPR016181" FT /db_xref="UniProtKB/TrEMBL:Q57025" FT /citation=[3] FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA99048.1" FT /translation="MTPQSMRELVICRASDADVLQLARCDFSFEVTAELEEPFDDMRSV FT PVKPPYLKNYGFDADELVEHMNNSAGALFVARADNCLVGYLAVSQSWNEYAVIDDIAVD FT VPYRGSGVSRLLMDAAVDWARNVPSAGVRLETQSVNLAACRFYRRYGFRLGGYDRYLYR FT GLHPGSREVALFWYLSF" FT promoter complement(7510..7640) FT /phenotype="resistance to streptothricins and to kanamycin/ FT neomycin" FT /function="promotes expression of sat3 and aphA genes" FT /note="This promoter is part of the resistance-gene-module FT common to pIE639 and pIE1107. It is not present in FT RSF1010." FT /citation=[3] FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT RBS complement(7529..7532) FT /citation=[1] FT -10_signal complement(7544..7549) FT /citation=[1] FT -35_signal complement(7566..7571) FT /citation=[1] XX SQ Sequence 8520 BP; 1854 A; 2512 C; 2521 G; 1633 T; 0 other; acatgaggca tgttccgcgt ccttgcaata ctgtgtttac atacagtcta tcgcttagcg 60 gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct agacattgcc agccagtgcc 120 cgtcactccc gtactaactg tcacgaaccc ctgcaataac tgtcacgccc ccctgcaata 180 actgtcacga acccctgcaa taactgtcac gcccccaaac ctgcaaaccc agcaggggcg 240 ggggctggcg gggtgttgga aaaatccatc catgattatc taagaataat ccactaggcg 300 cggttatcag cgcccttgcc caatatgccc ggccagaggc cggatagctg gtctattcgc 360 tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg 420 ctaaacccca caccaaaccc cgcagaaata cgctggagcg cttttagccg ctttagcggc 480 cttttcccta cccgaagggt gggggcggtg tgcagccccg aaggggccat tcaggggcta 540 ttctgaccgc cttgcgcttg gctatccttg cggctccggc tgaccatgct cctgtggctg 600 ctgtttctgc ttgggtggaa gtccgaacag tgcccgatca tggtcgcgct ctaggtagct 660 gtccaaccca tcgagcaggc gctgttctgg ccactcgccg ttttcaactt tgccgagtat 720 ccatgcacct accagcactt tgcgccgggt gttttccttg cgcttttgct gctgctcccg 780 tgctctgatc cgttggatct cggcgttgat tcgtgcccgt tgttcttcca gcttggcgag 840 ccgttccgct gtttttgcca ttttgataat cctcttggtt atgtgcttgt cattatgcta 900 tcatcggagc acagacaccg caaaccccga ccgtcatttt acgggagggc gcacttaccg 960 gtttctcttc aagaaactgc cctgggggca ccccttcggg gctgtgcgct ctccgagggc 1020 cattgcatgg agctaaaccc ctatggccat ctatcacctt acggccaaga ccggcagccg 1080 gaacggcggc caatcggcca aggcgaaagc cgattacatc cagcgggagg gccgttactc 1140 ccgcgaccgt gaagaggtgc tgcacaccca gtccggccac ctgcccgagt gggccgagcg 1200 tcccgccgac tactgggacg gtgccgacct gtacgagcgg gccaacggtc gcttattcaa 1260 agagattgag tttgccctgc cggtcgaact gaccctagat cagcagcggg agctggtgga 1320 cgaattcgcc cggcatctga ctgagggcga gcgcctgccg tatacgctgg ccatccatgc 1380 cggtaacggt gagaaccccc attgccactt gatgatctcc gagcggaaga acgacggtat 1440 cgagcgcccg gctgaccagt ggtttaagcg gtacaacggc aaacagccag agcggggcgg 1500 ggcgcagaag tccgagagcc tgaagcccaa ggcatggctt gagcagaccc gcgaggcatg 1560 gagccactac gccaaccgcg cccttgaaca ggccgggcac gaggcccgca ttgaccatcg 1620 cacccttgag gcgcagggca ttgagcgctt gccgggcatc catcttggcc ctaacgtggt 1680 ggagatggag agccggggca tccgcaccga gagggccgat atcgccctag ccatcgacac 1740 ggccaacggc cagatcatcg acttacaaga ataccgggag gttatcgagc atgagcgcga 1800 tagacagagt gaggaaatcc agagggatca acgagttagc ggcagagata gagccgctgg 1860 cccagagcat ggcgacactg gcggacgaag cccggcagcg gatcgccgag gtacagcagg 1920 ccagcgagga gcaggccgcg agctggacga gccagcaaca gcaagccatg agcgcatggc 1980 ggcaggcagc aaaggacatg agggcagccg caggggaact cgccaaggcc ggccagacgg 2040 cccggagcgc cgcccgtggc tggacatgga ggttgtgggc cggggtcttg atcgcttccg 2100 tgatgcctat tctggcgctg ctgatcgcat catggctttg gctggagccg caaatcatcg 2160 agcaacaggg gagcatatgg ctgattttca agctcaagtg aaaggtgacc gcaccgcgca 2220 agccatcgcc cggcagctca aggccatggg ctgcgaccgg tacgacatcg gcatacggga 2280 tgccgccagc ggcaagatga tgaaccggga atggacaccg caggaagtgc agcagaacgc 2340 cgcctggctc aagcgcatga atgcccaggg caacgatatt tacattcgcc ccgccgagca 2400 ggcccggcat ggtctggtgc tagttgacga cctcagcagc gacgatctgg acgccatgaa 2460 gcaggagggc cgggagcctg ccgccatcat cgagaccagc cccaagaatt atcaggcatg 2520 ggtgaaggtg gctcaggatg ccccagcaga tcacaggggc gtaatcgccc ggaagctggc 2580 ccgggagtat gacgccgacc cggcaagcgc cgacagccgt cactatggcc gtctggcagg 2640 ctttaccaac cgcaaggaca agtacaccag ccgcaccggc tatcagccgt gggtgctgtg 2700 ccgggagtcc agcggcaaga gtgccaccgc agggccggag ctgatgcagc aggccgggca 2760 ggtgttggac agcatcgagc gccgacagga acggacggca cgactggccg agatcaccgc 2820 cccgcagagt gtgcgtcggt atcgccgcag cgtcgtggac gactaccgca gcgaaatggc 2880 cgggctggtc aagcggtacg gtgatgacct cagcaagtgc gacttcatcg cggccatgaa 2940 gctggccagc aagggccggg aggcggacga gatcggcaag gccatggccg aggctagccc 3000 cgctatcatg gagcgcaagg cgggccatga ggccgattac atcaagcgta cggtgcagaa 3060 ggtgatggag cttccgcagg tgcaggaggc ccgcgccgag ctggcgaagc agacccagaa 3120 gcagcgcagc aaggggccag atctaagcat gtaatgcttg ttttaataaa acaaaactgc 3180 tatattgtca aaacaatttg actttatagt aggattaaaa aatggatata ggaaaggcaa 3240 aagagtattt tgggcttgga ttgattcagg gtgccgtggt gagatcgtca agtgttctgc 3300 ataacgggtg gactatcgag ctgagcggca acattggcag cgcacaacct accttacaca 3360 ccgccagagg tgatgtgcgg cagttcaaga cactggatgc ggcggccaag gtcgtgcgag 3420 aaattgggct gcgagagtgg cgtgttatca ctgactaggc ggcgcatggc ggcgcatccc 3480 cgccagcgct tgaccatcaa ccgcctgtaa aggaggcaaa ccaatggcta ctcataagcc 3540 tatcgatatt ctggcaagtt tcagagagct accgccgccg attgactacg tactgcccaa 3600 catggtggcc ggtaccgtgg gggcgctggt gtcccccggt ggggccggca agtctatgtt 3660 ggccctacag ctggccgcac agatcgcagg cggccccgac ctgctggacg tgggagagct 3720 gcccaccggc ccggtgatct acttgcccgc cgaagacccg cccaccgcta tacatcaccg 3780 cctgcacgca ttgggggcgc acctgaccga cgagcaacgg caagtcgtgg ctgacggtct 3840 gctaatccag ccgctgatcg gcagcctgcc caacatcatg gccccggagt ggttcgacgg 3900 cctcaagcgc gccgccgagg gccgccgcct gatggtgttg gatacgctgc gtcggttcca 3960 tatcgaggaa gagaacgcca gtggccccat ggcgcaggtg attggccgca tggaggccat 4020 cgccgccgat accgggtgtt ctatcgtgtt cctgcaccat gccagcaagg gcgcggccat 4080 gatgggcgca ggcgaccagc agcaggccag ccggggatcg tccgtgctgg tggataacat 4140 ccgctggcag tcctacctgt ccggcatgac ggccgccgag gccgaggaat ggggcgtgga 4200 cgattcacag cggccctatt tcgtccgctt tggtgtcagc aaggccaact atggggcgcc 4260 gttcaaagac cgctggttca ggcggcatga cggcggggtg ctcaaacccg ccgtgctgga 4320 aaagcagcgc aaaagcaagg gggtgcgccg tggtgaagcc taagaacaag tacagtctca 4380 gccacgtccg gcacgacccg gcgcactgtc tggctcccgg cctgttccgt gccctcaagc 4440 ggggcgagcg caagcgcagc aagctggacg tgacgtatga ctacggcgac ggcaagcgga 4500 tcgagttcaa ggggccggag ccgctgggtg ctgatgatct gcgcatcctg caagggctgg 4560 tggcaatggc tgggcctaat ggcctagtgc ttagcccaga acccaagacc gaaggcgggc 4620 agcagctccg gttgttcttg gagcccaagt gggaggccgt taccgctgat gccatggtgg 4680 ttaagggcag ctatcgggcg ctggcccgtg aaatcgggta tgcagaggat ggcggcagcc 4740 agttcaaggc aatccgagag tgcattgagc gcctgtggac agtctccatc atcgcccaaa 4800 atggccgcaa gcggcagggg ttccggctgc ttgccgagta cgccagcgac gaggcaggcg 4860 ggcgtctgta cgtggctctg aaccccttaa ttgctcaggc cgtcatgggt ggtggccagc 4920 atgtgcgcat cagcatggac gaggtgcgag cgctggacag cgaagcggcc cgcctgctgc 4980 atcagcgcct gtgtggctgg atcgaccccg gcaagaccgg caaggcggcc atagataccc 5040 tgtgcggcta tgtctggccg tcagaggcca gtggttcgac catgcgcaag cgccgccagc 5100 gggtgcgcga ggcgttgccg gagctggttg cgctgggttg gacggtaacc gagttcgcgg 5160 cgggcaagta cgacatcacc cggcccaagg cggcaggctg accccctgtt taacagtcac 5220 gccctccccc ggcgtaactg tcacgccccc ggcgtaactg tcacgccccc ccggcgtaac 5280 tgtcacgaac ccccggcgta actgtcacgc ccctaaacct gcaaacccag gaaggggggg 5340 agctggtggg gtgttggaaa aatccatcca tgattatcca agaataatcc actaggcgcg 5400 gttaacagcg ccctagtggg gcgctgtttg cccttgctta aaatgccggc cagaggccgg 5460 ataacttctc tatccgctgc gctaggctgc acacgccccc ccaccgctgc gcggcagggg 5520 gaaaggcggg caaagcccgc taaaccccac accaaattct gcgggaatac gctggagcga 5580 ttttagccgc tttagcggcc ttttccctac ccgaagggtg ggggcggtgt gcagccccga 5640 aggggccatt tagcggccat ttcagcggct gtgatgctga tagtggccgt ggtgtccctt 5700 acgatatggg agtaatcagg cttgccgaaa agggctttga gtggatctgt catggctgat 5760 ctccaaacat atcaagcgtg tctgtatccc actcagtaat caataagaag aaaaatcctt 5820 tttaaatcaa ataattgaaa cagacgccct caccgatacc acgaaaaata ccatggcaag 5880 ccaatcggtc tgctcaccag tgactcaggc gagcagggac cgcgccgaca ggtttgcaca 5940 ggacgttcga atgtgcaggt gcgatcctac tgtgacgtca tcatcaaatg aaggtttggc 6000 aaaacagcgg cgttcatgca cggggaggcc acctgccacc gcactgcact gcactgcact 6060 gcactgcacc gcatccagtt cgagcatcgt agcctcaaaa cagctcatcc agcatcaagt 6120 gaaatgacag cttccgtcga tctacatcag cgatgccata ttgctccacg aatcgttcct 6180 ggggcaaaga gccaaactca cctagacggt tccagagtat ggctatgtcc tggtatcgat 6240 ctgcaacgcc tgatcttcca acatcaatgc aaccagtcac cttgccttcg cgaacgaaga 6300 gattctcgag agcagaatct ccgtgcgttg tgacaggatc gggcgtgaac ggaagaagat 6360 tatgcatcgc atcccatacc tgctcagccg cccagccttt ccgttcgtca tcaaagtcat 6420 cgacctcgac catgcccgct tcgatgcgtt tgcgcgcaag agcaagtctg tagatgtgat 6480 tgctgttgaa agggcattca atcgttggga tctcatgcag gcggcgcaag aaatcagcta 6540 gcgcgtctgc gatggcaagg caagcatcgg gattcgcctc cagctcttcg tagaccgtcc 6600 tgccaggcat tgccgttgtc agcaaccacg cttcgtcgag ggtgcgaaca aagttgacga 6660 cggttggcac agggatacgt tcggcaagcc agcgtagcct gaccatttca tcggtcacat 6720 catcagcaac gtcgtactta cctcgcttca aaaacaaatc aggagctttt ggtttcccat 6780 aaagtcgata aacattgctg ccggattcac caattgtgat aggcgcccat tggtatcctt 6840 ccagactggt tgccatgctc gcaggcatgg gaataggtgc acagacttgc tgtcgtcgcc 6900 actcactcat tcataactcc gctcatctgg ccgagtgccg tttcccggac ggccaaagtt 6960 tgtcatttaa aaactcaaat accagaacag agctacctct cggctgcccg gatgcaggcc 7020 acgatacagg tagcgatcat aaccacctaa ccggaaaccg tatcgtcggt aaaagcgaca 7080 tgcggcgaga ttaacggact gcgtctccag acgtacgcct gccgacggca catttcgtgc 7140 ccagtccaca gctgcatcca tcagcaagcg cgaaacgcca ctcccccgat agggcacatc 7200 gaccgcgata tcatcgatga cggcatattc gttccagctt tgagacacgg ccaagtagcc 7260 aacaaggcaa ttgtccgccc gagccacaaa caacgcccca gcagagttgt tcatatgctc 7320 gaccaactca tcggcatcaa agccatagtt cttgaggtag ggcggcttga ctggaacgga 7380 ccgcatgtca tcgaacggct cttcgagctc agctgtgacc tcgaaagaga aatcgcaccg 7440 cgcaagctga agaacgtcgg catcgcttgc acgacagatg accaattcac gcattgactg 7500 tggcgtcatg acggggattc cttgtcacag acctttatta taaataatat aataattttc 7560 tttattttca atagttttaa aaaataaatc atagcaatgc catgaaaaat accatgctca 7620 gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac agctccatag 7680 gccgctttcc tggctttgct tccagatgta tgctcttctg ctcctgcagc taatggatca 7740 ccgcaaacag gttactcgcc tggggattcc ctttcgaccc gagcatccgt atgagactca 7800 tgctcgatta ttattattat agaagccccc atgaataaat cgctcatcat tttcggcatc 7860 gtcaacataa cctcggacag tttctccgat ggaggccggt atctggcgcc agacgcagcc 7920 attgcgcagg cgcgtaagct gatggccgag ggggcagatg tgatcgacct cggtccggca 7980 tccagcaatc ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg tatcgcgccg 8040 gtgctggacg cgctcaaggc agatggcatt cccgtctcgc tcgacagtta tcaacccgcg 8100 acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg cggttttcca 8160 gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt tatgcattcg 8220 gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat ggatcacatt 8280 gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc 8340 cttgtccttg atcccggcat ggggtttttt ctgggggctg ctcccgaaac ctcgctctcg 8400 gtgctggcgc ggttcgatga attgcggctg cgcttcgatt tgccggtgct tctgtctgtt 8460 tcgcgcaaat cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt cggggccgcc 8520 //