ID M29725; SV 1; linear; genomic DNA; STD; UNC; 4408 BP. XX AC M29725; XX DT 12-JUN-1991 (Rel. 28, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 4) XX DE Plasmid pLS1 (from Streptococcus agalactiae), complete genome. XX KW complete genome; repA gene; repB gene; replication protein A; KW replication protein B; tet gene; tetracycline resistance. XX OS Plasmid pLS1 OC other sequences; plasmids. OG Plasmid pLS1 XX RN [1] RP 1-4408 RX DOI; 10.1016/0022-2836(86)90026-4. RX PUBMED; 2438417. RA Lacks S.A., Lopez P., Greenberg B., Espinosa M.; RT "Identification and analysis of genes for tetracycline resistance and RT replication functions in the broad-host-range plasmid pLS1"; RL J. Mol. Biol. 192(4):753-765(1986). XX FH Key Location/Qualifiers FH FT source 1..4408 FT /organism="Plasmid pLS1" FT /plasmid="pLS1" FT /host="Streptococcus agalactiae" FT /mol_type="genomic DNA" FT /db_xref="taxon:2595" FT gene 655..792 FT /gene="repA" FT CDS 655..792 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="replication protein A" FT /db_xref="GOA:P13920" FT /db_xref="InterPro:IPR002145" FT /db_xref="InterPro:IPR010985" FT /db_xref="InterPro:IPR013321" FT /db_xref="PDB:1B01" FT /db_xref="PDB:1EA4" FT /db_xref="PDB:2CPG" FT /db_xref="UniProtKB/Swiss-Prot:P13920" FT /protein_id="AAA98165.1" FT /translation="MKKRLTITLSESVLENLEKMAREMGLSKSAMISVALENYKKGQEK FT " FT gene 853..1551 FT /gene="repB" FT CDS 853..1485 FT /codon_start=1 FT /transl_table=11 FT /gene="repB" FT /product="replication protein B" FT /db_xref="GOA:P13921" FT /db_xref="InterPro:IPR002631" FT /db_xref="PDB:3DKX" FT /db_xref="PDB:3DKY" FT /db_xref="UniProtKB/Swiss-Prot:P13921" FT /protein_id="AAA98166.1" FT /translation="MAKEKARYFTFLLYPESIPSDWELKLETLGVPMAISPLHDKDKSS FT IKGQKYKKAHYHVLYIAKNPVTADSVRKKIKLLLGEKSLAMVQVVLNVENMYLYLTHES FT KDAIAKKKHVYDKADIKLINNFDIDRYVTLDVEEKTELFNVVVSLIRAYTLQNIFDLYD FT FIDENGETYGLTINLVNEVIAGKTGFMKLLFDGAYQRSKRGTKNEER" FT terminator 1531..1551 FT /gene="repB" FT gene 1736..3157 FT /gene="tet" FT CDS 1736..3112 FT /codon_start=1 FT /transl_table=11 FT /gene="tet" FT /product="tetracycline resistance protein" FT /db_xref="GOA:P0A4K6" FT /db_xref="InterPro:IPR001411" FT /db_xref="InterPro:IPR011701" FT /db_xref="InterPro:IPR016196" FT /db_xref="InterPro:IPR020846" FT /db_xref="UniProtKB/Swiss-Prot:P0A4K6" FT /protein_id="AAA98167.1" FT /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN FT KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS FT LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA FT HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS FT ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY FT MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA FT SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT FT GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF FT " FT terminator 3119..3157 FT /gene="tet" FT CDS 3185..4084 FT /codon_start=1 FT /transl_table=11 FT /note="ORFD" FT /db_xref="UniProtKB/TrEMBL:Q52049" FT /protein_id="AAA98168.1" FT /translation="MADMELKEELLEKYHAPLFVDERTGELNNDTEAFWHEKEFADMFE FT VQSPIRETTNQEKMDWLRKQYQEELKKLESSKKPLEDDLSHLEELLDKKTKEYIKIDSE FT ASERASELSKAEGYINTLENHSKSLEAKIECLESDNLQLEKQKATKLEAKALNESELRE FT LKPKKNFLGKEHYELSPEQFEGLKAEVYRSRTLLHHKDIELEEAKRQVSLRASKNYFTA FT SLERAKEKAKGESIDRLKSEIKRLKNENSILRQQNDKMLGKLRELMPDKAFKNLLSELK FT AIKPIVNIIKKAIEKSLF" XX SQ Sequence 4408 BP; 1414 A; 672 C; 969 G; 1353 T; 0 other; ctgcagaagt agtcgctgat tggctaattc agcgtatcaa agacaaaggc gaccaaaaat 60 agcttgagtt cttttagaac aaaaaagaaa gacagtagtt gcacctactg tctttctttt 120 gcgttgtgct tttagttcct cgaactttta gcgtcaagca tattatatca tggggcgaga 180 aattctgtca aaataatgct ataatgcttt tgaggcacct cagcgatacg gtcggtggtg 240 tgaatctcat ttacgtaggg cgactggaaa cggatagctc aaagggcgcg tttgagtgtc 300 gggtgtggga ctgccttcag cttcgggctg taaagacccc tgatactttt gaatgagatg 360 accctttggg gtcttttttg tttttttagg gagatgttgt gggggatttt ttctccgaaa 420 aaatctaaaa tatggggggg ctactacgac cccccctata gtgccgagtg ccaaaatcaa 480 aaaaaaaacg cctttagcct tagagctgca agggtttgag gctcgtcaaa tctcggcgac 540 ttttcggcga cttttcggcg actttttaga gattttttgg gaaaaatacg aaaaagattg 600 cattgagtgc acggttatgc tactatagtt ttataaaatt ttgagaggtg acgcatgaaa 660 aaaagattga cgataacatt aagtgaatcg gtacttgaaa atcttgaaaa aatggcaaga 720 gagatggggt tatcaaaatc tgcaatgatt tctgttgcct tggaaaatta caagaaaggt 780 caagaaaaat aaaaaaagcc gtgctggcag gcactggcta aagtcaaaca tttcttgggt 840 atattatact ttatggctaa agaaaaagca agatacttca cttttttact ttatcctgaa 900 tcaattccaa gcgactggga gctgaaactt gaaacgcttg gagtgccgat ggcaattagt 960 ccattgcatg ataaggataa gagtagtatc aaaggacaaa aatataagaa agctcattat 1020 catgtgcttt atatagctaa aaatccagtt actgcagata gtgtacgtaa aaagattaaa 1080 ttattgcttg gtgaaaaaag tcttgcaatg gtgcaggttg ttctcaatgt cgaaaatatg 1140 tatttgtatt taacgcacga gagcaaggac gctattgcta agaagaaaca tgtttatgat 1200 aaggctgata taaagctaat caataatttt gatattgacc gttatgtgac gttagatgtc 1260 gaggaaaaga ccgaactttt caatgtggtt gtatcgctta ttcgtgcgta cactctccaa 1320 aatatttttg atttgtatga tttcattgac gaaaatggag aaacttatgg gttgactata 1380 aatttggtta acgaagttat tgcagggaaa actggtttta tgaaattgtt gtttgacgga 1440 gcttatcaac gtagtaagcg tggaacaaag aacgaagaga gataaaaagt tgatctttgt 1500 gaaaactaca gaaagtaaag aatgaaaaga gtaatgctaa catagcatta cggattttat 1560 gaccgatgat gaagaaaaga atttgaaact tagtttatat gtggtaaaat gttttaatca 1620 agtttaggag gaattaatta tgaagtgtaa ttaatgtaac agggttcaat taaaagaggg 1680 aagcgtatca ttaaccctat aaactacgtc tgccctcatt attggagggt gaaatgtgaa 1740 tacatcctat tcacaatcga atttacgaca caaccaaatt ttaatttggc tttgcatttt 1800 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa 1860 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt 1920 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact 1980 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt 2040 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc 2100 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg 2160 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat 2220 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt 2280 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa 2340 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag 2400 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa 2460 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt 2520 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat 2580 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg 2640 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc 2700 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt 2760 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct 2820 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc 2880 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc 2940 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt 3000 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat 3060 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa 3120 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaagag 3180 ggcaatggct gatatggaac tcaaagagga acttcttgaa aaatatcatg caccgctttt 3240 tgttgatgag agaacaggcg agttgaacaa tgacacggaa gctttttggc atgaaaaaga 3300 gtttgctgat atgtttgaag ttcaatctcc gatacgtgaa acaactaacc aagaaaaaat 3360 ggactggtta agaaaacagt accaagaaga gctgaaaaaa ctagaatcgt ctaaaaagcc 3420 cctagaagac gatttaagcc atttagaaga gttgcttgat aaaaagacca aggaatatat 3480 taaaatcgat tctgaggcct ctgagagggc ctcagagcta tctaaagccg agggatatat 3540 aaatacccta gaaaatcatt cgaagagctt agaagcgaaa atagagtgtt tagagagtga 3600 taatctacaa ttggaaaaac aaaaggcgac aaaactcgaa gcgaaagcgt tgaacgagag 3660 tgagttgcga gaactaaagc ctaagaagaa ttttctagga aaagagcatt atgagttaag 3720 tcctgaacaa tttgaagggt tgaaggcaga agtttatcgt agtagaactc tattgcacca 3780 caaagatatt gaactggagg aagcaaaacg tcaagtatct ctgagagcct ctaaaaacta 3840 ttttacagct agtttagagc gagctaagga aaaagctaaa ggtgagagta tagaccgtct 3900 taaaagcgaa ataaagcgac taaaaaacga aaattcaatt ttacgtcagc aaaatgacaa 3960 gatgctaggg aaattaagag agttaatgcc tgataaagcc tttaagaatt tgttatcaga 4020 acttaaggcg attaagccaa tcgtgaatat aattaaaaag gctattgaaa agagcttgtt 4080 ctgagcgatt tatgccgtga aagctatttg acaataagca gtgacagagt acgctaggac 4140 gtgccgagcc gaaaggcttt agcgtttcgg acggacacgg acaaaggacg gcagtcactg 4200 gttacttgtt gtcaaataga ccatggaata aaaagcgtca aaagtcttga gtggatgata 4260 ccctatggta ctctattcgc cttttgactt ttttgctata atttaagtgt cgccagttct 4320 tccgtcaggt aatgcgaact tagactggag gtgagcgttg tgaagacatt cctcgagctt 4380 gtctttgtcc cttttgtggt tggcgttg 4408 //