ID   M13761; SV 1; linear; genomic DNA; STD; UNC; 2246 BP.
XX
AC   M13761;
XX
DT   16-JUL-1988 (Rel. 16, Created)
DT   15-JAN-2007 (Rel. 90, Last updated, Version 3)
XX
DE   Plasmid pIM13 (from B.subtilis), complete genome, encoding a possible
DE   replication protein and macrolide-lincosamide-streptogramin B resistance
DE   protein, complete cds.
XX
KW   complete genome; lincosamide resistance; macrolide resistance;
KW   replication protein; streptogramin resistance.
XX
OS   Plasmid pIM13
OC   other sequences; plasmids.
OG   Plasmid pIM13
XX
RN   [1]
RP   1-2246
RX   PUBMED; 3087948.
RA   Monod M., Denoya C., Dubnau D.;
RT   "Sequence and properties of pIM13, a macrolide-lincosamide-streptogramin B
RT   resistance plasmid from Bacillus subtilis";
RL   J. Bacteriol. 167(1):138-147(1986).
XX
CC   Draft entry and computer-readable sequence for [1] kindly provided
CC   by D.Dubnau, 05-OCT-1987.
CC   Regions of dyad symmetry are located at positions 73-96, 821-870,
CC   1211-1235, 1282-1307, and 2188-2220.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2246
FT                   /organism="Plasmid pIM13"
FT                   /plasmid="pIM13"
FT                   /host="Bacillus subtilis"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:2568"
FT   CDS             389..829
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="replication protein"
FT                   /note="putative"
FT                   /db_xref="GOA:P13968"
FT                   /db_xref="InterPro:IPR001808"
FT                   /db_xref="InterPro:IPR008813"
FT                   /db_xref="InterPro:IPR011991"
FT                   /db_xref="UniProtKB/Swiss-Prot:P13968"
FT                   /protein_id="AAA98135.1"
FT                   /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
FT                   LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
FT                   IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
FT   gene            1453..2187
FT                   /gene="mls"
FT   CDS             1453..2187
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="mls"
FT                   /product="macrolide-lincosamide-streptogramin B-resistance
FT                   protein"
FT                   /db_xref="GOA:P13956"
FT                   /db_xref="InterPro:IPR001737"
FT                   /db_xref="InterPro:IPR020596"
FT                   /db_xref="InterPro:IPR020598"
FT                   /db_xref="InterPro:IPR023165"
FT                   /db_xref="PDB:1QAM"
FT                   /db_xref="PDB:1QAN"
FT                   /db_xref="PDB:1QAO"
FT                   /db_xref="PDB:1QAQ"
FT                   /db_xref="PDB:2ERC"
FT                   /db_xref="UniProtKB/Swiss-Prot:P13956"
FT                   /protein_id="AAA98136.1"
FT                   /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
FT                   LELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNIPY
FT                   NISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSLALFLMAEVDISILSMVPREYF
FT                   HPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
FT                   DLNNISFEQFLSLFNSYKLFNK"
XX
SQ   Sequence 2246 BP; 862 A; 341 C; 359 G; 684 T; 0 other;
     atcgattatg tcttttgcgc attcacttct tttctatata aatatgagcg aagcgaataa        60
     gcgtcggaaa agcagcaaaa agtttccttt ttgctgttgg agcatggggg ttcagggggt       120
     gcagtatctg acgtcaatgc cgagcgaaag cgagccgaag ggtagcattt acgttagata       180
     accccctgat atgctccgac gctttatata gaaaagaaga ttcaactagg taaaatctta       240
     atataggttg agatgataag gtttataagg aatttgtttg ttctaatttt tcactcattt       300
     tgttctaatt tcttttaaca aatgttcttt tttttttaga acagttatga tatagttaga       360
     atagtttaaa ataaggagtg agaaaaagat gaaagaaaga tatggaacag tctataaagg       420
     ctctcagagg ctcatagacg aagaaagtgg agaagtcata gaggtagaca agttataccg       480
     taaacaaacg tctggtaact tcgtaaaggc atatatagtg caattaataa gtatgttaga       540
     tatgattggc ggaaaaaaac ttaaaatcgt taactatatc ctagataatg tccacttaag       600
     taacaataca atgatagcta caacaagaga aatagcaaaa gctacaggaa caagtctaca       660
     aacagtaata acaacactta aaatcttaga agaaggaaat attataaaaa gaaaaactgg       720
     agtattaatg ttaaaccctg aactactaat gagaggcgac gaccaaaaac aaaaatacct       780
     cttactcgaa tttgggaact ttgagcaaga ggcaaatgaa atagattgac ctcccaataa       840
     caccacgtag ttattgggag gtcaatctat gaaatgcgat taagcttttt ctaattcaca       900
     taagcgtgca ggtttaaagt acataaaaaa tataatgaaa aaaagcatca ttatactaac       960
     gttataccaa cattatactc tcattatact aattgcttat tccaatttcc tattggttgg      1020
     aaccaacagg cgttagtgtg ttgttgagtt ggtactttca tgggattaat cccatgaaac      1080
     ccccaaccaa ctcgccaaag ctttggctaa cacacacgcc attccaacca atagttttct      1140
     cggcataaag ccatgctctg acgcttaaat gcactaatgc cttaaaaaaa cattaaagtc      1200
     taacacacta gacttattta cttcgtaatt aagtcgttaa accgtgtgct ctacgaccaa      1260
     aagtataaaa cctttaagaa ctttcttttt tcttgtaaaa aaagaaacta gataaatctc      1320
     tcatatcttt tattcaataa tcgcatcaga ttgcagtata aatttaacga tcactcatca      1380
     tgttcatatt tatcagagct cgtgctataa ttatactaat tttataagga ggaaaaaata      1440
     aagagggtta taatgaacga gaaaaatata aaacacagtc aaaactttat tacttcaaaa      1500
     cataatatag ataaaataat gacaaatata agattaaatg aacatgataa tatctttgaa      1560
     atcggctcag gaaaagggca ttttaccctt gaattagtac agaggtgtaa tttcgtaact      1620
     gccattgaaa tagaccataa attatgcaaa actacagaaa ataaacttgt tgatcacgat      1680
     aatttccaag ttttaaacaa ggatatattg cagtttaaat ttcctaaaaa ccaatcctat      1740
     aaaatatttg gtaatatacc ttataacata agtacggata taatacgcaa aattgttttt      1800
     gatagtatag ctgatgagat ttatttaatc gtggaatacg ggtttgctaa aagattatta      1860
     aatacaaaac gctcattggc attattttta atggcagaag ttgatatttc tatattaagt      1920
     atggttccaa gagaatattt tcatcctaaa cctaaagtga atagctcact tatcagatta      1980
     aatagaaaaa aatcaagaat atcacacaaa gataaacaga agtataatta tttcgttatg      2040
     aaatgggtta acaaagaata caagaaaata tttacaaaaa atcaatttaa caattcctta      2100
     aaacatgcag gaattgacga tttaaacaat attagctttg aacaattctt atctcttttc      2160
     aatagctata aattatttaa taagtaagtt aagggatgca taaactgcat cccttaactt      2220
     gtttttcgtg tacctatttt ttgtga                                           2246
//