ID L75827; SV 1; linear; genomic DNA; STD; UNC; 2846 BP. XX AC L75827; XX DT 26-JAN-1996 (Rel. 46, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 6) XX DE Plasmid pWC1, complete sequence. XX KW . XX OS Plasmid pWC1 OC other sequences; plasmids. OG Plasmid pWC1 XX RN [1] RP 1-2846 RX DOI; 10.1006/plas.1996.0015. RX PUBMED; 8700966. RA Pillidge C.J., Cambourn W.M., Pearce L.E.; RT "Nucleotide sequence and analysis of pWC1, a pC194-type rolling circle RT replicon in Lactococcus lactis"; RL Plasmid 35(2):131-140(1996). XX RN [2] RP 1-2846 RA Cambourn W.M., Pillidge C.J., Willis K.J., Pearce L.E.; RT "A new type of rolling circle replicon in Lactococcus lactis"; RL Unpublished. XX RN [3] RP 1-2846 RA Pillidge C.J.; RT ; RL Submitted (19-JAN-1996) to the INSDC. RL New Zealand Dairy Research Institute, Private Bag 11029, Palmerston North, RL New Zealand XX DR RFAM; RF01742; lactis-plasmid. XX FH Key Location/Qualifiers FH FT source 1..2846 FT /organism="Plasmid pWC1" FT /plasmid="pWC1" FT /host="Lactococcus lactis" FT /mol_type="genomic DNA" FT /db_xref="taxon:45203" FT repeat_region 463..580 FT /rpt_type=DIRECT FT /note="putative; DR1" FT repeat_region 676..793 FT /rpt_type=DIRECT FT /note="putative; DR1" FT protein_bind 784..808 FT /standard_name="double strand origin" FT /function="site of single strand nicking by replication FT protein" FT /bound_moiety="RepB protein" FT /note="label:DSO" FT /note="High sequence homology to DSOs from several other FT pC194-type plasm...; putative" FT -35_signal 902..907 FT -10_signal 924..932 FT RBS 981..988 FT /note="label:RBS" FT /note="Translation of RepB is probably coupled to the FT immediately preceding small peptide (CDS positions 1001 - FT 1039); putative" FT gene 1036..1986 FT /gene="repB" FT CDS 1036..1986 FT /codon_start=1 FT /transl_table=11 FT /gene="repB" FT /product="replication protein" FT /function="replication of (+)DNA strand of plasmid" FT /note="Produces single-strand nick at a specific site in FT plasmid, resulting in replication by rolling-circle FT mechanism; high similarity to other pC194-type plasmid FT replication proteins; putative" FT /db_xref="GOA:Q52233" FT /db_xref="InterPro:IPR000989" FT /db_xref="UniProtKB/TrEMBL:Q52233" FT /protein_id="AAD10221.1" FT /translation="MKEYFQGDEFKDISKNGKDRKWKERKINNLNLAKIFDSLDYPDSF FT IFNIKSCAEYLNFKRSSDGSLRLFQMYTCKNKQCAICSWRRSMKYQVQISKIVEEAMIR FT KPKGRFLFLTLTVENVSGEGLNNELSLLSEAFNRLMKYKKVSKNILGFLRATEVTINES FT MDTYHPHIHVLLFISPTYFKNKNNYISQDEWTELWKKSAKLDYRPIVDVRSIKPKNEKT FT SDIRSAILETAKYPVKPMELNYDSAKVVDDLQKGLYRKRQIAFGGLFKQIKKELELDDI FT ENGDLINIGDEENPISDGEIISVLWNHERQNYYVR" XX SQ Sequence 2846 BP; 959 A; 401 C; 438 G; 1048 T; 0 other; gcatgcaaat gaatagtgag gttagagcag gtcataaata acggtcatat ttaaaagcta 60 aaattgtacg ttttagtcaa ttacatacaa gcctatttaa gggttttttc ttttatccat 120 gataattatt actaactcac ttttaaaatc gcttagaatg caaactatga gcttttagga 180 ctattcaaag cgtttagact aaacaaagta gccacgaccg ttagggagtt ctttttagtg 240 gcagaatgta tctaacataa aattagagtg tacaagcgga gcgacattat catccaggga 300 tataattacc ttatttatac ttatagtaat taataagttt tttattttca gatttatatt 360 ctttgttcat tgggttttct ccttttttca gggtggtatg tactgtctct aattttttgt 420 tgaatattaa aaaagttaga tatataatta tgagcagaat gtttagtcta cttactgggt 480 tgaaattttg actttcttca acagtcaaag aaactattat gttgaaggtt gtttgaatca 540 attttaagca gatcggtttt atatgagttg ctgctttctt gttatcttgg tgtgaatttt 600 taatcttcaa aatattaatt ggtaatgatc ggaagatcat tacaaaaatt agatatataa 660 atagatatag gagggttagt ctacttactg ggttgaaatt ttgactttct tcaacagtca 720 aagaaactat tatgttgaag gttgtttgaa tcaattttaa gcagatcggt tttatatgag 780 ttgctgcttt cttcttatct tgatactatt ggaaataaca agattttcaa aaaaaaccgt 840 atatcctttt aatttaaagg gtttaacggt tttttttatc attatttagt ggaaaaaaaa 900 taaaacaaaa acttctctac ttatgttata atgattttat aatttccgac caaagaaatt 960 aagaccaaaa tactataaga aaaggagaag tctcggtttt ttgtatacaa tattgttgag 1020 actattatat cataaatgaa agaatatttt caaggggatg agtttaaaga catctctaaa 1080 aatggtaaag atagaaaatg gaaagaacgg aaaattaata atttaaatct tgcgaaaatc 1140 ttcgattcgc tggattatcc ggatagtttt atttttaata ttaaaagttg cgcagagtat 1200 ttgaacttta aacgttcaag tgatggatct cttcgtttat tccaaatgta cacttgtaaa 1260 aataaacaat gtgctatttg ctcttggagg cggtcaatga agtatcaagt tcagatctca 1320 aaaattgtcg aagaagcaat gattagaaaa cccaagggac gctttttgtt cttgacgtta 1380 acggttgaaa atgtttctgg agaaggtttg aataacgaac tatctcttct ttctgaagcc 1440 ttcaatcggt tgatgaaata taaaaaagtt tctaaaaata ttcttggatt cctgagagca 1500 actgaagtaa cgataaacga atcaatggat acttatcacc ctcatattca cgttttactt 1560 tttattagtc cgacatactt taaaaataaa aataattata tttcacaaga tgaatggaca 1620 gagctttgga aaaaatcagc aaaactagat tatcgtccaa ttgttgatgt tcgatcaatt 1680 aaaccaaaaa atgaaaaaac atcagacatt cgttctgcga tcttggaaac agcaaaatat 1740 ccagttaagc caatggaatt gaattatgat tcagcaaaag tggttgatga cctccaaaaa 1800 ggactttatc ggaaacgaca aatcgctttt ggtggacttt ttaaacaaat caaaaaagaa 1860 ctagaattag atgatatcga aaatggtgac ttaattaata ttggtgatga agaaaatcca 1920 atttctgacg gagaaattat ttctgtactt tggaatcatg aacgccaaaa ttattacgtg 1980 agataatagg caaaaatggg aaaaactatc tatgcgagtt ttcctcatca tcaatttaat 2040 tctttggttc tctttttcat ttacacgaaa ttttttctgt atacttctct cacgattttc 2100 aattattttt atcttgtcaa cctttcaaac tactgtcaac tctaaaagct caaaataggg 2160 ggttagtgtc tttagtgtct ttagtgaact tagtaagtgt ggctacactt acagtaaaaa 2220 gtattcatct ttcactcaca aaacctaaaa tttccaattt tatttttgtt cgtttcagac 2280 gaatattttt atttttggtg gtgtccccca atcccccttt ttgaaaaaaa ttagagctta 2340 atttcaaatt cttttaagac atcttcgtat aaatctttga cttcaaaaca caaattttta 2400 acttcatctt cggtcaaagt ttgaacatat ttttttgcgt taatgtaatt atttaattct 2460 tctttatcaa taaaatctaa ttgttcatgt atcatttctt ctaacatatc tatcaatact 2520 ttttttggag ttggaaattt atttgtcagt tgaattgctt ctaaaatttc acgtccataa 2580 aattcagttt ctgtaaaatc ttcgccttga tagttaatca tcattcattt tctcctttga 2640 ttttattgtt tgaaagttta tcatttttta tcacattttc taattgttca tcagaaattt 2700 tatcaaagcc ccaagaattt aaatagccga ttgaataatc ttttgtgtca atatttaaat 2760 gattagcaac gacaaaagcg gtagattctg cttccaattc ttgtaaagca tattttttag 2820 aaccgaacag ttttgaccta ttttta 2846 //