ID   K00053; SV 1; linear; genomic DNA; STD; UNC; 1855 BP.
XX
AC   K00053;
XX
DT   13-JUN-1985 (Rel. 06, Created)
DT   15-JAN-2007 (Rel. 90, Last updated, Version 5)
XX
DE   Plasmid Rts1 (from E.coli) fragment (complete mini-Rts1) coding for RepA
DE   protein.
XX
KW   complete genome; incompatibility determinant; origin of replication;
KW   repA gene.
XX
OS   Plasmid Rts1
OC   other sequences; plasmids.
OG   Plasmid Rts1
XX
RN   [1]
RP   1-496
RX   PUBMED; 6309744.
RA   Kamio Y., Terawaki Y.;
RT   "Nucleotide sequence of an incompatibility region of mini-Rts1 that
RT   contains five direct repeats";
RL   J. Bacteriol. 155(3):1185-1191(1983).
XX
RN   [2]
RP   497-1855
RX   PUBMED; 6325393.
RA   Kamio Y., Tabuchi A., Itoh Y., Katagiri H., Terawaki Y.;
RT   "Complete nucleotide sequence of mini-Rts1 and its copy mutant";
RL   J. Bacteriol. 158(1):307-312(1984).
XX
CC   This fragment of plasmid Rts1 represents the complete genome of the
CC   mini-Rts1 plasmid [1].  Mini-Rts1 is capable of autonomous
CC   replication.
CC   [1] notes two clusters of direct repeats (48-241 and 1205-1288)
CC   that flank the open reading frame, a structure characteristic of
CC   other stringent replication plasmids.  The direct repeat consensus
CC   is 'ttccccyyrrcacacacc'; there are a total of 8 copies.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1855
FT                   /organism="Plasmid Rts1"
FT                   /plasmid="Rts1"
FT                   /host="Escherichia coli"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:2507"
FT   gene            complement(265..1131)
FT                   /gene="repA"
FT   CDS             complement(265..1131)
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="repA"
FT                   /product="replication protein"
FT                   /db_xref="GOA:P05833"
FT                   /db_xref="UniProtKB/Swiss-Prot:P05833"
FT                   /protein_id="AAA91589.1"
FT                   /translation="METQLVISDVLFGNTEEKQKPLTVNELNTIQPVAFMRLGLFVPKP
FT                   SRSSDYSPMIDVSELSSTFEFARLEGFTDIKITGERLDMDTDFKVWIGIVKAFSKYGIS
FT                   SNRIKLKFSEFAKDCGFPGKKLDKKLRAHIDESLRKIRGKSISFKRGKDSQSAYHTGLI
FT                   KIAYFNADTDVVELEADERLWELYYFDYRVVLQLHAIKALPRLEVAQALYTFLASLPSN
FT                   PAPISFERLRERLSLISQVKEQNRIIKKAITKLIDIGNLDASMVKKGQENYLIIHKRSP
FT                   KLSVINE"
XX
SQ   Sequence 1855 BP; 501 A; 460 C; 349 G; 545 T; 0 other;
     gaattctcgt taaacccctt ggcacacacc gaacgaaagc caattccttc cccctgacac        60
     acactttacc ggtgataatt acgccccgtg atactcactc ggagggaaaa ccaccacttt       120
     tccccctgac gcacaccttg ccagtggttc cccctgacac acaccttgcc agagaggcga       180
     tcagttcccc ctgacacaca ccttgccatg actgttaacc cccctgacac acacttttca       240
     ttgacagcca aaccacaggc acacttattc gttgattaca cttagctttg gacttcgctt       300
     gtgaatgatg aggtaattct cttgtccttt tttcaccata gaagcgtcca aattgccgat       360
     atctatcagc ttagtaatcg cttttttgat gattcgattc tgttctttaa cctgactgat       420
     cagagacaac ctttcacgta ggcgttcaaa agagatagga gccgggttac ttggaaggct       480
     tgcaaggaag gtatacaggg cttgtgcaac ttctagacgt ggaagggctt ttattgcatg       540
     tagttgaaga acaacacgat aatcaaagta gtataactcc cataatcgct cgtctgcttc       600
     cagttccaca acgtctgtat cggcattgaa ataggctatc tttatgaggc cggtatgata       660
     tgcagattgt gaatctttgc ctcgcttaaa tgagatcgat ttccccctga ttttacgaag       720
     cgattcatct atatgcgctc tcagtttctt gtccagtttt ttaccgggga aaccacaatc       780
     ttttgcgaac tcagaaaact ttagtttgat gcggtttgac gaaatcccgt acttgctgaa       840
     cgctttgact atgccgatcc acaccttgaa atcagtatcc atatcaagac gttcaccagt       900
     gatttttatg tcagtaaacc cctcaagtct tgcaaattca aaggtagaac ttaattcact       960
     gacatcaatc atcgggctgt agtcagatga ccttgatggt ttaggcacaa acaggccaag      1020
     gcgcataaag gccactggtt gaattgtatt cagttcatta actgttaatg gtttttgttt      1080
     ttcttccgtg ttaccaaata acacatcaga tataactagt tgagtttcca taataaaata      1140
     attccccccc acgtaaatgc tacgattgtg gaaaagtatc acattttccc cgtgacgccc      1200
     accttttttc ccctcagcac acacccaaat ccccgtaaca cacactttct tcccctcagc      1260
     acacaccttt ttcccctcag cacacaccga aaaacgttgt aagccttgtg gcagtaaggc      1320
     tacagaccac ggggatcttt ttggttttat ctggatctct ctatggatct attattggat      1380
     ctacacggtg gatatgtgga taactttaaa tgatggtaat gtggaggaat atctgacgcg      1440
     ctacgcttgt ccaaccgctg ccgggcaatg aaaggtaaac ccattcatta cccgacaacg      1500
     gttcccggaa ctaccccaaa gccatcggtt ctaaccgtaa gtatacagtt tcatagataa      1560
     atttaagaag tccgttgaag tatacgcgtt gatgcttatc tgctgcccct gtagcctgat      1620
     cgattaatac ctccagaccg ttcttaggct gattaagtga gacattattt agtagattat      1680
     attgctcaac gaatccatct gtaccaatag cctctatcac tatcaatctg aaagcaggat      1740
     ctctcaacag agccagttca tgaggtgtat agattttgat gattttcatc tgagtttgtt      1800
     ccctttctgg taagacaggt ttatcgaacc gcatcagaat acatctgata agctt           1855
//