ID J01764; SV 1; circular; genomic DNA; STD; UNC; 4439 BP. XX AC J01764; J01765; XX DT 13-JUN-1985 (Rel. 06, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 8) XX DE Plasmid pT181, complete genome. XX KW drug resistance protein; origin of replication; repC gene; KW tetracycline resistance. XX OS Plasmid pT181 OC other sequences; plasmids. OG Plasmid pT181 XX RN [1] RP 1-875,3883-4439 RX DOI; 10.1073/pnas.79.13.4108. RX PUBMED; 6287465. RA Novick R.P., Adler G.K., Majumder S., Khan S.A., Carleton S., RA Rosenblum W.D., Iordanescu S.M.; RT "Coding sequence for the pT181 repC product: a plasmid-coded protein RT uniquely required for replication"; RL Proc. Natl. Acad. Sci. U.S.A. 79(13):4108-4112(1982). XX RN [2] RP 4060-4439 RX DOI; 10.1073/pnas.79.15.4580. RX PUBMED; 6956881. RA Khan S.A., Adler G.K., Novick R.P.; RT "Functional origin of replication of pT181 plasmid DNA is contained within RT a 168-base-pair segment"; RL Proc. Natl. Acad. Sci. U.S.A. 79(15):4580-4584(1982). XX RN [3] RP 1-4439,1-4439 RX DOI; 10.1016/0147-619X(83)90039-2. RX PUBMED; 6657777. RA Khan S.A., Novick R.P.; RT "Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid RT from Staphylococcus aureus"; RL Plasmid 10(3):251-259(1983). XX RN [4] RP 2640-3740 RX PUBMED; 3584064. RA Gennaro M.L., Kornblum J., Novick R.P.; RT "A site-specific recombination function in Staphylococcus aureus plasmids"; RL J. Bacteriol. 169(6):2601-2610(1987). XX DR RFAM; RF00242; ctRNA_pT181. XX CC [4] revises [3]. CC pT181 occurs naturally in Staphylococcus aureus and encodes CC inducible resistance to tetracycline. It contains four open reading CC frames encoding four putative polypeptides A, 37.5 kDa; B, 35 kDa; CC C, 23 kDa, and D, 18 kDa [3]. Polypeptide A is the repC protein [2] CC (bases 4289-794). Polypeptides B and D (bases 956-1843 and CC 1800-2258, respectively) are involved in tetracycline resistance CC [3]. There is no known function for polypeptide C and deletion of a CC large portion of it causes no noticeable changes [3]. The CC recombination site RS-A consists of 24 bp of identity (the core, CC positions 2448-2472) flanked by some 50 base pairs of decreasing CC homology with other small Staphylococcal plasmids [4]. The CC recombination at RS-A is recA-independent and is mediated by a CC plasmid-encoded, transacting protein, Pre (plasmid recombination) CC [4]. Pre-mediated recombination is site specific in that it occurs CC within the core sequence of RS-A in a recA host [4]. Recombination CC also occurs between two intramolecular RS-A sites [4]. Pre-RS-A is CC not involved in plasmid maintenance [4]. CC Replication, originating in a 168 bp span, proceeds CC unidirectionally and clockwise (anticlockwise as the map is CC conventionally drawn) [2]. CC Polypeptide C has been changed to the longer Pre protein by CC revisions given in [1]. XX FH Key Location/Qualifiers FH FT source 1..4439 FT /organism="Plasmid pT181" FT /plasmid="pT181" FT /mol_type="genomic DNA" FT /db_xref="taxon:2638" FT CDS 956..1843 FT /codon_start=1 FT /transl_table=11 FT /note="polypeptide B (ttg start codon)" FT /db_xref="GOA:P02983" FT /db_xref="InterPro:IPR001411" FT /db_xref="InterPro:IPR011701" FT /db_xref="InterPro:IPR016196" FT /db_xref="InterPro:IPR020846" FT /db_xref="UniProtKB/Swiss-Prot:P02983" FT /protein_id="AAA26034.1" FT /translation="MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHFN FT TTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFF FT ILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIA FT HYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYN FT WTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGWANIFYSSWFYINGAL FT YDENYLSCKCSDNR" FT CDS 1800..2258 FT /codon_start=1 FT /transl_table=11 FT /note="polypeptide D" FT /db_xref="GOA:P02983" FT /db_xref="InterPro:IPR001411" FT /db_xref="InterPro:IPR011701" FT /db_xref="InterPro:IPR016196" FT /db_xref="InterPro:IPR020846" FT /db_xref="UniProtKB/Swiss-Prot:P02983" FT /protein_id="AAA26035.1" FT /translation="MMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVF FT ILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGELSFTKTVISKIVSSSLSEEEVASG FT MSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNIL" FT misc_feature 2448..2472 FT /note="RS-A site [4]" FT mRNA 2478..>3762 FT /note="Pre mRNA" FT CDS 2521..3762 FT /codon_start=1 FT /transl_table=11 FT /note="Pre protein (plasmid recombination)" FT /db_xref="GOA:P03864" FT /db_xref="InterPro:IPR001668" FT /db_xref="UniProtKB/Swiss-Prot:P03864" FT /protein_id="AAA26036.1" FT /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY FT DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF FT EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ FT DRFNEHVKQRGYGLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND FT KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQEAGNVVISQKDFNEFQKQIKAA FT QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE FT IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ FT EQQKNQKRDRGMHL" FT misc_feature 3760..3993 FT /note="RS-B site [4]" FT rep_origin 4257..4424 FT /note="replication origin" FT CDS join(4289..4439,1..794) FT /codon_start=1 FT /transl_table=11 FT /note="repC protein (polypeptide A)" FT /db_xref="GOA:P03064" FT /db_xref="InterPro:IPR003491" FT /db_xref="UniProtKB/Swiss-Prot:P03064" FT /protein_id="AAA26033.1" FT /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF FT DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD FT RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEEDLSDYYAMSDKAVK FT KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV FT DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP FT VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK" XX SQ Sequence 4439 BP; 1652 A; 542 C; 793 G; 1452 T; 0 other; ttggaaatct caaccgagac aacgctcaag ccctttctaa atttatgagt gtagagcccc 60 aaataagact ttgggatatt cttcaaacaa agtttaaagc taaagcactt caagaaaaag 120 tttatattga atatgacaaa gtgaaagcag atagttggga tagacgtaat atgcgtattg 180 aatttaatcc aaacaaactt acacgagatg aaatgatttg gttaaaacaa aatataataa 240 gctacatgga agatgacggt tttacaagat tagatttagc ctttgatttt gaagaagatt 300 tgagtgacta ctatgcaatg tctgataaag cagttaagaa aactattttt tatggtcgta 360 atggtaagcc agaaacaaaa tattttggcg tgagagatag taatagattt attagaattt 420 ataataaaaa gcaagaacgt aaagataatg cagatgctga agttatgtct gaacatttat 480 ggcgtgtaga aatcgaactt aaaagagata tggtggatta ctggaatgat tgctttagtg 540 atttacatat cttgcaacca gattggaaaa ctatccaacg cactgcggat agagcaatag 600 tttttatgtt attgagtgat gaagaagaat ggggaaagct tcacagaaat tctagaacaa 660 aatataagaa tttgataaaa gaaatttcgc cagtcgattt aacggactta atgaaatcga 720 ctttaaaagc gaacgaaaaa caattgcaaa aacaaatcga tttttggcaa catgaattta 780 aattttggaa atagtgtaca tattaatatt actgaacaaa aatgatatat ttaaactatt 840 ctaatttagg aggatttttt tatgaagtgt ctatttaaaa atttggggaa tttatatgag 900 gtgaaagaat aatttacccc tataaacttt agtcacctca agtaaagagg taaaattgtt 960 tagtttatat aaaaaattta aaggtttgtt ttatagcgtt ttattttggc tttgtattct 1020 ttcatttttt agtgtattaa atgaaatggt tttaaatgtt tctttacctg atattgcaaa 1080 tcattttaat actactcctg gaattacaaa ctgggtaaac actgcatata tgttaacttt 1140 ttcgatagga acagcagtat atggaaaatt atctgattat ataaatataa aaaaattgtt 1200 aattattggt attagtttga gctgtcttgg ttcattgatt gcttttattg gtcacaatca 1260 cttttttatt ttgatttttg gtaggttagt acaaggagta ggatctgctg cattcccttc 1320 actgattatg gtggttgtag ctagaaatat tacaagaaaa aaacaaggca aagcctttgg 1380 ttttatagga tcaattgtag ctttaggtga agggttaggt ccttcaatag ggggaataat 1440 agcacattat attcattggt cttacctact tatacttcct atgattacaa tagtaactat 1500 accttttctt attaaagtaa tggtacctgg taaatcaaca aaaaatacat tagatatcgt 1560 aggtattgtt ttaatgtcta taagtattat atgttttatg ttatttacga caaattataa 1620 ttggactttt ttaatactct tcacaatctt ttttgtgatt tttattaaac atatttcaag 1680 agtttctaac ccttttatta atcctaaact agggaaaaac attccgttta tgcttggttt 1740 gttttctggt tgggctaata ttttctatag tagctggttt tatatcaatg gtgccttata 1800 tgatgaaaac tatttatcat gtaaatgtag cgacaatagg taatagtgtt atttttcctg 1860 gaaccatgag tgttattgtt tttggttatt ttggtggttt tttagtggat agaaaaggat 1920 cattatttgt ttttatttta ggatcattgt ctatctctat aagtttttta actattgcat 1980 tttttgttga gtttagtatg tggttgacta cttttatgtt tatatttgtt atgggcgaat 2040 tatcttttac taaaacagtt atatcaaaaa tagtatcaag tagtctttct gaagaagaag 2100 ttgcttctgg aatgagtttg ctaaatttca caagtttttt atcagaggga acaggtatag 2160 caattgtagg aggtttattg tcactacaat tgattaatcg taaactagtt ctggaattta 2220 taaattattc ttctggagtg tatagtaata ttctgtagcc atggctaacc ttattattta 2280 agttgtcttt tgacgattat tgtatttaaa acgttctgaa aagcagtttg aatagttata 2340 ttatattttg gtttagaact atgagtggct agcattttgc cactcatttt ttgcgttagc 2400 aaaaacaggt ttaagcctcg cagagcacac gtattaacga cttattaaaa ataagtctag 2460 tgtgttagac ttaaactatt aaatacacat aaaacctttg tgcttaggag tgatttttat 2520 atgtcttatt ccattgttag agtttcaaaa gttaaatctg gaacaaatac aacgggcata 2580 caaaaacatg ttcaaagaga aaataataat tatgaaaatg aagatataga ccatagtaaa 2640 acttacttaa attatgattt ggtaaatgct aataaacaga attttaataa cttgattgat 2700 gaaaaaatcg aacagaatta tacaggcaaa agaaaaatta gaacagacgc gattaaacac 2760 attgatggtt taattacatc agacaatgat ttctttgata atcaaacgcc agaagataca 2820 aagcagtttt ttgaatatgc taaagagttt ttagaacaag aatacggtaa agataattta 2880 ttatatgcaa cagttcacat ggacgaaaaa acaccacata tgcattatgg cgttgttcca 2940 ataactgatg atggtcgttt aagtgctaaa gaagttgtag gtaataaaaa agctttaaca 3000 gcgtttcaag atagatttaa tgagcatgtt aaacaacgag gatatggttt agaacgtggg 3060 caatcaagac aagtaacaaa tgctaaacat gagcaaataa gtcagtataa acaaaaaaca 3120 gaatatcata agcaagaata tgaacgtgag agccaaaaaa cagaccatat aaagcaaaag 3180 aacgataaat taatgcaaga gtaccaaaaa tcgttaaata cgcttaaaaa gcctataaat 3240 gttccgtatg agcaagaaac tgaaaaagta ggtggtttat ttagcaaaga gatacaagaa 3300 gctggaaatg ttgtaataag ccaaaaagat ttcaatgaat ttcagaaaca gataaaagct 3360 gctcaagata tttcggaaga ttacgagtat ataaagtctg gtagagcctt agatgataaa 3420 gataaggaaa tacgagagaa agatgattta ttaaataaag cagttgagcg tattgaaaac 3480 gcagacgata attttaacca actttacgaa aatgcaaagc cacttaaaga gaatatagaa 3540 atagcgttaa agcttttaaa aatcttacta aaagagttag aacgagtttt aggaagaaat 3600 acctttgcgg aaagagttaa taagttaaca gaagatgaac caaaactaaa tggtttagca 3660 ggaaacttag ataaaaaaat gaatccagaa ttatattcag aacaggaaca gcaacaagaa 3720 caacaaaaga atcaaaaacg agatagaggt atgcacttat agaacatgca tttatgccga 3780 gaaaacttat tggttggaat gggctatgtg ttagctaact tgttagcgag ttggttggac 3840 ttgaattggg attaatccca agaaagtacc aactcaacaa cacataaagc cctgtaggtt 3900 ccgaccaata aggaaattgg aataaagcaa taaaaggagt tgaagaaatg aaattcagag 3960 aagcctttga gaattttata acaagtaagt atgtacttgg tgttttagta gttttaactg 4020 tttaccagat aatacaaatg cttaaataaa aaaagacttg atctgattag accaaatctt 4080 ttgatagtgt tatattaata acaaaataaa aaggagtcgc tcacgccctg accaaagttt 4140 gtgaacgaca tcattcaaag aaaaaaacac tgagttgttt ttataatctt gtatatttag 4200 atattaaacg atatttaaat atacatcaag atatatattt gggtgagcga ttccttaaac 4260 gaaattgaga ttaaggagtc gattttttat gtataaaaac aatcatgcaa atcattcaaa 4320 tcatttggaa aatcacgatt tagacaattt ttctaaaacc ggctactcta atagccggtt 4380 ggacgcacat actgtgtgca tatctgatcc aaaattaagt tttgatgcaa tgacgatcg 4439 //