ID CP000041; SV 1; circular; genomic DNA; STD; PRO; 5108 BP. XX AC CP000041; XX PR Project:PRJNA12468; XX DT 07-JUN-2005 (Rel. 84, Created) DT 08-MAR-2010 (Rel. 104, Last updated, Version 2) XX DE Bacillus cereus E33L plasmid pE33L5, complete sequence. XX KW . XX OS Bacillus cereus E33L OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; OC Bacillus cereus group. OG Plasmid pE33L5 XX RN [1] RP 1-5108 RG Joint Genome Institute Microbial Sequencing, Finishing and Annotation Team RA ; RT "Complete genome sequence of Bacillus cereus E33L"; RL Unpublished. XX RN [2] RP 1-5108 RG Joint Genome Institute Microbial Sequencing, Finishing and Annotation Team RA ; RT ; RL Submitted (08-NOV-2004) to the INSDC. RL Joint Genome Institute, Department of Energy, 2800 Mitchell Drive, Walnut RL Creek, CA 94598, USA XX DR GR; CP000041_GR. DR RFAM; RF01691; Bacillus-plasmid. XX CC Bacillus cereus E33L was originally isolated from a swab of the CC carcass of a dead zebra suspected of having died of anthrax in the CC Etosha National Park, Namibia, April 1996. The identification of CC the isolate as B. cereus was made by P.C.B. Turnbull and given the CC designator E33L. B. anthracis was not isolated from the swab. CC Phylogenetically, the E33L isolate is the closest Bacillus isolate CC to the B. anthracis branch so far identified. B. cereus is CC ubiquitous in the environment and has been associated with many CC different types of infections since 1898. B.cereus associated CC pathology includes both diarrheal-type and emetic-type food CC poisoning (Bacillus cereus toxins. Turnbull, PCB. Pharmac Ther CC 13:453-505 1981).The pathogenicity of B. cereus is due to the CC production of several toxins, including emetic toxin, enterotoxins, CC phospholipases, proteases and hemolysins (Bacillus cereus and CC related species. Drobniewski, FA. Clin Microbiol Rev 6(4):324-338 CC 1993). Plasmid and fosmid libraries were prepared at the Joint CC Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing CC was performed at the JGI Production Genomics Facility (JGI-PGF) in CC Walnut Creek, CA to a coverage of 9x. Finishing was performed at CC JGI-LANL and 5 plasmids ranging in size from 5 kb to 467kb were CC identified. One of these, designated pE33L5, is described in this CC record. Gene predictions were obtained using Glimmer. Basic CC analysis of the gene predictions was performed by comparing coding CC sequences against the COGs, Pfam, BLOCKS and Prodom databases. Gene CC definitions and gene names were added manually by a team of CC annotators at JGI-LANL, using BLAST results in addition to CC information from the basic analysis. Five open reading frames were CC identified on this 5108 bp sequence. XX FH Key Location/Qualifiers FH FT source 1..5108 FT /organism="Bacillus cereus E33L" FT /plasmid="pE33L5" FT /strain="E33L" FT /mol_type="genomic DNA" FT /db_xref="taxon:288681" FT gene complement(join(5070..5108,1..372)) FT /locus_tag="pE33L5_0001" FT CDS complement(join(5070..5108,1..372)) FT /codon_start=1 FT /transl_table=11 FT /locus_tag="pE33L5_0001" FT /product="conserved hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:Q4V105" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAY60602.1" FT /translation="MKKYISIFSLFVMLLTGCQFQKEENIPGIEQLESVKEIKISNFKK FT LDSLNENFNKSFSKKNEIGTFKNAMKTAKNQKQNITEYDYNIQIIFNDDSNKALHIAKT FT NEKKIILKYIGENTDTYVIDAKDSKELITLIY" FT gene 480..1385 FT /locus_tag="pE33L5_0002" FT CDS 480..1385 FT /codon_start=1 FT /transl_table=11 FT /locus_tag="pE33L5_0002" FT /product="conserved hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:Q4V104" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAY60603.1" FT /translation="MGILFKKGLLSTVCVTAMASLLTFSPLVTSAKTDTTQVKTASKHE FT HSVQKGEITLKYTAPDVDLSTVTPEQKETLEKIGWKVENGILVQYRSIPEEVKKAIDLG FT YLEPLEEVNHQKHNAPTTFSNHEDHTGHEEIEEVLIVNGEKVEIKDGRFDVKGNPDTLE FT VQIDKDTKQTVQKSEDGKYHLVLEQNLSEVIEKMDNHEGHDNMTHADQSLLNPFSDVTY FT GYGDTYRNGDWVHCNRFNGPLSDNRHLRKTNPQAIINFYMSDCDLGALRYCLNHANCNQ FT KYRAAYCSWKLGHSEKYHKH" FT gene 2363..3598 FT /gene="mob1" FT /locus_tag="pE33L5_0003" FT CDS 2363..3598 FT /codon_start=1 FT /transl_table=11 FT /gene="mob1" FT /locus_tag="pE33L5_0003" FT /product="mobilization protein" FT /db_xref="GOA:Q4V103" FT /db_xref="InterPro:IPR001668" FT /db_xref="UniProtKB/TrEMBL:Q4V103" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAY60604.1" FT /translation="MSYAICRMQKFKMRDVKGIQIHNQREKESHTNHDIESERTHLNYD FT LHNDGHIDYLKTVKDKIEQNVETNRAIRKDAVVMCEFVVTSDKEFFDVLDMVDPVQQKV FT FFKEAYNFLKNRYGEQNIVHAAVHLDEKTPHMHVGMVPVTEENKLSAKQIFNRKELVSL FT QDDFHAHMVEKGFYLERGVSSDKKHVEMRKLKAMTAKEEIKALEKEMGQCLQKKEVVNK FT QMEQLESRLNQLQNAVKSTNKVDKMEVKHKGGILRSKTVEMSVEDFEELKTKAKASEAF FT KRENMTLHRKNEALQASNTDLHTTVKRLEKENEGLKPYKARYERLAKLMDEMNKFYEKF FT IPKELSKFHEIIGFCKRKVNASINRFSSLRYSEKELNENEKRGYETASKFLENQRKEQK FT QQRKERGDEREL" FT gene 3595..3810 FT /locus_tag="pE33L5_0004" FT CDS 3595..3810 FT /codon_start=1 FT /transl_table=11 FT /locus_tag="pE33L5_0004" FT /product="hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:Q4V102" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAY60605.1" FT /translation="MIGELSMKKLKRNAIQCRKCGDVIESKFTHDFKYCSCGSVGVDGG FT LEYARMIGNLEDIIELYEFEGNEKQE" FT gene complement(3831..4421) FT /gene="rep" FT /locus_tag="pE33L5_0005" FT CDS complement(3831..4421) FT /codon_start=1 FT /transl_table=11 FT /gene="rep" FT /locus_tag="pE33L5_0005" FT /product="replication protein" FT /db_xref="GOA:Q4V101" FT /db_xref="InterPro:IPR008813" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/TrEMBL:Q4V101" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAY60606.1" FT /translation="MIHRRVKNMALEVLQLDKEHEEKKITVELTSDELKKIQKLLEIEE FT EKEKTEEQREEEKKNYNFIQLYRDNMPELRWLMTKQPFASSLLFFILEHMDNRNALACS FT YSVFEDYFGKSRMTIYRAIKTLEENGFIDVLKMGTSNIYLVNENLAWTDKNTSKKFAKY FT DGKILVSKKENKDYQYRSQFDRFKALRERENLK" XX SQ Sequence 5108 BP; 1821 A; 707 C; 871 G; 1709 T; 0 other; atcaataaca tatgtgtctg tattttcacc aatgtatttt aaaataattt tcttttcatt 60 agttttagct atatgtaaag ctttattaga atcatcatta aatattattt gtatattata 120 gtcatattct gttatatttt gcttttggtt tttagcggtt ttcatggcat ttttaaaagt 180 accaatttcg tttttcttag aaaaagattt attaaaattt tcatttaaag aatctagttt 240 tttaaaatta gatattttta tttcttttac actttctaac tgttcaattc caggtatgtt 300 ttcttctttt tgaaattgac atcctgttaa tagcattaca aacaatgaaa atatagaaat 360 atactttttc atattagccc tccattttag atttattatt ataatattaa aacttaaacg 420 aaatttccaa taattgtgtt atactagtat tgttttaaaa ataatatatt tgtaagggga 480 tgggaatttt gtttaaaaaa ggattattaa gtactgtttg tgtaacagca atggcaagtt 540 tacttacttt ttctccattg gtaacttcag ctaaaacaga taccacccaa gttaagacag 600 catctaaaca tgaacattct gttcaaaaag gagaaattac acttaaatac acagcacctg 660 atgttgattt aagtacagta actccagaac aaaaagaaac tttagaaaaa ataggttgga 720 aagtagaaaa tggtattctt gtacaatatc gttcaattcc agaagaagtt aaaaaagcta 780 tagacctagg gtatcttgaa cctttagaag aagtaaatca tcaaaaacat aatgcaccta 840 ctactttttc aaaccatgaa gatcatacag gtcatgaaga aattgaagaa gttttaattg 900 ttaatggtga aaaggtagaa ataaaagatg gacgttttga tgtaaaaggt aatcctgata 960 cattagaagt tcaaattgat aaagatacta aacagacagt tcaaaaaagt gaagatggta 1020 aatatcattt agtattagaa caaaatttaa gtgaagtaat tgaaaagatg gacaatcatg 1080 agggtcatga taatatgact catgctgatc aatcactttt aaatcctttt tcagatgtaa 1140 cttatggtta tggagatact tatagaaatg gtgattgggt acattgtaac agatttaatg 1200 gtcctctttc tgataataga catttacgga aaactaatcc tcaagctatt atcaattttt 1260 atatgagtga ttgtgatctt ggtgcactta gatattgttt aaatcatgca aattgtaatc 1320 aaaaatatag agctgcatat tgttcttgga aacttggaca tagcgaaaaa tatcataaac 1380 attaaaaata tcaaacaaaa aagaggggaa atctcctctt tttatttttg atatttttaa 1440 ataaataatt cttttcaatt atggaaggga gatgttttat tgtatgagtt tgacactatt 1500 aacaacaaca ttactactta ttttaatttc taattttatt attaaaaatc tgttaaaaaa 1560 caaaataaaa aaaactactc ttaattttat tagtagtgta gtagtattac caggaatctc 1620 tttattttgg ttaattactt atacttgtga tttaccattt aatatttttt taagttttta 1680 ttttccatta attattacga tatttttaat tctaatgtta ataattaatt taattaagtt 1740 gattaaaaat gttgatgtat agttttataa ttatttgaag tgatatttat tttaaggggt 1800 ctctcgaatt gtctgaggga tttttttgtt tttcgatgtt ttatatgtgt aggagaatat 1860 acaagtatta caaatgaatt tgaaacgttt taagggtata tagcaagttt taatcgtatt 1920 cgttttatag aaaaaaagaa ttaaaaaggc tagtgctgaa atagataaga ggtagttgca 1980 tggcgtgtgt caacggtacc ggaccgaacc cgaaagggtg cgggaggata aggagttgac 2040 acacttacta tgcgaccctc tgggcattga acaaactacg gtttgttcct gccaaccggc 2100 gagggagccc ctcaaggatt gaggggttgg ggagttcgat taagggggtc tggggaagag 2160 ttccccagtg ggtttgggca acgcccaagg tttttattcg gcaagccgaa tcctccgtag 2220 gagcacactc tttacgatag taaagtatag tgtgctatac tttacatgga agtccaccga 2280 aaaacagtag agaagtatat atactttata tggttctagg gactgataag gacgtagaac 2340 catatggaaa agaggcgaaa aaatgagtta tgcaatttgc cgaatgcaga aatttaaaat 2400 gcgagatgtg aagggaattc agattcataa tcagagagag aaagagagtc acacaaatca 2460 tgatattgaa tcggagcgaa cacatcttaa ttatgattta cataatgatg gccacattga 2520 ttatttaaaa acagtgaaag acaagattga acaaaatgta gagaccaatc gggcgattcg 2580 aaaagatgcg gttgtgatgt gtgagtttgt ggtaacaagc gataaagaat tttttgatgt 2640 gctagatatg gttgatccag ttcaacaaaa ggtgtttttc aaagaagctt ataatttttt 2700 aaaaaataga tatggggaac aaaatattgt gcatgcagcg gtgcatttgg atgagaaaac 2760 acctcatatg cacgttggca tggttccagt aacggaagaa aataagttat cagccaaaca 2820 aatttttaac cgaaaagaat tggtttcact ccaggacgat tttcacgctc acatggtaga 2880 aaagggcttt tatttggaac gtggtgtatc aagtgacaaa aaacatgtag aaatgcgaaa 2940 acttaaggca atgaccgcaa aggaagagat aaaagcttta gaaaaagaga tgggacaatg 3000 cctacagaaa aaagaagtag taaataaaca aatggaacaa ttagaatctc gtttgaatca 3060 attacaaaat gcagtgaaat caacaaataa agtcgataag atggaagtta aacataaagg 3120 tggtatttta cgctctaaaa ccgttgaaat gagcgtagaa gactttgaag agctcaaaac 3180 taaggcgaag gcatcagaag cctttaaacg tgaaaatatg acgttacatc gcaagaatga 3240 agcattacaa gcaagtaata cagatttaca tacaacagtg aaacgattgg aaaaagaaaa 3300 tgaagggctg aagccgtata aagccagata tgaacgtcta gcaaaattaa tggatgaaat 3360 gaataaattt tatgagaagt ttattccaaa agaactgtct aaatttcatg agattattgg 3420 tttttgtaag agaaaagtaa atgcatctat aaatagattt agttctctta gatattcgga 3480 aaaagaatta aatgaaaatg agaaaagagg ttatgaaaca gcatcaaagt ttctcgaaaa 3540 tcaacgaaaa gaacaaaaac aacaaagaaa agaacgtgga gatgaaagag aactttgata 3600 ggagagttgt caatgaaaaa gctaaagaga aatgcaattc aatgtcgaaa atgtggtgat 3660 gtaattgaat ccaagttcac tcatgatttt aagtattgtt catgtggatc agtgggagta 3720 gatggtggtt tggaatatgc tcgtatgatt ggaaacttag aagatatcat tgaattatat 3780 gaattcgagg gaaatgaaaa acaagagtaa tcaaataaaa atgattactc ttattttaga 3840 ttttctcttt ctcttaatgc tttgaatcta tcaaattggc ttcgatattg ataatcttta 3900 ttttcttttt tgctaacaag aattttacca tcatattttg cgaatttttt acttgtgttt 3960 ttatctgtcc aggcaaggtt ttcattgact aaataaatat ttgatgtacc catttttaat 4020 acgtcaataa aaccgttttc ttcaagggtt ttaatcgctc tataaattgt cattctcgat 4080 tttccgaaat aatcttcaaa aactgaatat gaacaggcta aagcattacg gttgtccata 4140 tgttctagta taaaaaataa caaactacta gcaaatggct gttttgtcat taaccaacgt 4200 aattcaggca tattatcacg atacaattga ataaagttat agttcttttt ttcttcttct 4260 ctttgttctt ctgtcttctc tttttcttcc tcaatctcta ataatttttg aatttttttc 4320 aattcatcac tagtcaattc aactgtaatc tttttttctt catgttcttt atccaattgc 4380 aacacctcta acgccatatt tttaaccctc ctatgtatca caagttatga tacatttatg 4440 tatcaacttt gttacatcgt ataacaaaaa gtataacacg taaagaataa tttgttacat 4500 gacgtaacat aaatgtatca caagctatgt tacatttatg tatcacaagc tatgttacat 4560 ttactcttta aaaaccctta tttatcaatg tttttcagcc tgtccctttc tatatctcct 4620 agtagggctt ttctctatgc aatggtcagg tagtattttt ttgagaaccc cactcaaaaa 4680 aatctccacc tttcctccat tgcatggaga aattcggctg gtttttagct ttcgccaagc 4740 cgaacatacc aaaaacaagg atttaggttg ttcgacttgg actagcaatt gtaccagccg 4800 aaccgcctag atccatcgac caaaacattc agctttttct atattcagtt acgttaagtt 4860 cgtggggttt agcccccaca cgactagcca gcattcacac ccaaaaacaa ggagaaaaat 4920 ttttaaaacc aaatactaga ttttggttta ttctctttta ataccgaaga ggccaaataa 4980 gcccctttaa tataaaaaaa gaccaacttt tagttggtct ttttttatat ttgattttgt 5040 gattcaaaaa attattttat taataacatt taataaataa gtgttattaa ttctttagaa 5100 tctttagc 5108 //