ID AF325537; SV 2; circular; genomic DNA; STD; PRO; 14155 BP. XX AC AF325537; XX DT 31-MAY-2001 (Rel. 67, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Acidithiobacillus caldus plasmid pTC-F14 replicon, complete sequence. XX KW . XX OS Acidithiobacillus caldus OC Bacteria; Proteobacteria; Gammaproteobacteria; Acidithiobacillales; OC Acidithiobacillaceae; Acidithiobacillus. OG Plasmid pTC-F14 XX RN [1] RP 1-14155 RX DOI; 10.1128/JB.183.11.3303-3309.2001. RX PUBMED; 11344137. RA Gardner M.N., Deane S.M., Rawlings D.E.; RT "Isolation of a new broad-host-range IncQ-like plasmid, pTC-F14, from the RT acidophilic bacterium Acidithiobacillus caldus and analysis of the plasmid RT replicon"; RL J. Bacteriol. 183(11):3303-3309(2001). XX RN [2] RP 1-14155 RX DOI; 10.1128/JB.185.20.6104-6111.2003. RX PUBMED; 14526022. RA van Zyl L.J., Deane S.M., Rawlings D.E.; RT "Analysis of the mobilization region of the broad-host-range IncQ-like RT plasmid pTC-F14 and its ability to interact with a related plasmid, RT pTF-FC2"; RL J. Bacteriol. 185(20):6104-6111(2003). XX RN [3] RP 1-14155 RA Gardner M.N., Deane S.M., Rawlings D.E.; RT ; RL Submitted (01-DEC-2000) to the INSDC. RL Department of Microbiology, University of Stellenbosch, Private Bag X1, RL Matieland, Western Cape Province 7602, South Africa XX RN [4] RC Sequence update by submitter RP 1-14155 RA Rawlings D.E., Deane S.M., Gardner M.N., van Zyl L.J., Goldschmidt G.K.; RT ; RL Submitted (14-APR-2003) to the INSDC. RL Department of Microbiology, University of Stellenbosch, Private Bag X1, RL Matieland, Western Cape Province 7602, South Africa XX DR RFAM; RF00043; Plasmid_R1162. XX CC On Apr 14, 2003 this sequence version replaced gi:14209910. XX FH Key Location/Qualifiers FH FT source 1..14155 FT /organism="Acidithiobacillus caldus" FT /plasmid="pTC-F14" FT /strain="F" FT /mol_type="genomic DNA" FT /note="IncQ-like plasmid" FT /db_xref="taxon:33059" FT gene complement(197..859) FT /gene="mobE" FT CDS complement(197..859) FT /codon_start=1 FT /transl_table=11 FT /gene="mobE" FT /product="mobilization protein of unknown function" FT /db_xref="UniProtKB/TrEMBL:Q840R7" FT /protein_id="AAP04743.1" FT /translation="MSDLKKAIGLITGREPTPEDINRVSAIAHALDIPQNDAMFPILVT FT LDAYYGTFSKLPKAMENTANKIASNAEEAAKGRISAASSELLSKAGQQLVDAFRSDLGK FT TLWSRSVWAGLVLVVVGIGSYAAGEVDIWWHSKEVRQVEQKLQRLKAEEAAIQTRIGGI FT VQECPAFDGIQSGPCVPIDVAANVANNFGYEKHDGKTVYYGRLDIGAIEQAETKQHQ" FT repeat_region 580..604 FT /rpt_type=INVERTED FT gene complement(852..1532) FT /gene="mobD" FT CDS complement(852..1532) FT /codon_start=1 FT /transl_table=11 FT /gene="mobD" FT /product="mobilization protein of unknown function" FT /db_xref="UniProtKB/TrEMBL:Q840R6" FT /protein_id="AAP04744.1" FT /translation="MSKSLNKILVVGGGKGGVGKSTVTMALVDALLSGSHSCVLVESDD FT SNPDTYKALKDLLPCHICNLDTEEGYIHLGNIIENHPKNSIVVNTAARATSALIQYGGI FT LNDVAQEQNRELIMLWPINRQRDSLELLKDFLETAKPYHATYAVLNTYFGAPEKFARFA FT NSTLKNRVSGTVIFPELNDLVADKLVDNRLSLGSADTKLSISERSALRRYREAAQTALK FT AAYE" FT gene complement(1529..1924) FT /gene="mobC" FT CDS complement(1529..1924) FT /codon_start=1 FT /transl_table=11 FT /gene="mobC" FT /product="histone-like mobilization protein" FT /db_xref="UniProtKB/TrEMBL:Q840R5" FT /protein_id="AAP04745.1" FT /translation="MASSKQLYDIDAYAKKARTALENLEKTQQPGEVTGKGGKADVIRA FT IKSDLEALIKKGYTSKQIADALKHDDVFGILPKTITEIVAGKKQNKSTVTRKKKSTTPA FT PTASGQANTAPIPQAGTFTVKPDSEDL" FT misc_feature 2026..2027 FT /note="proposed origin of transfer nic site; cleavage site" FT misc_feature 2027..2033 FT /note="proposed MobA binding site; conserved nucleotide FT hexamer; cleavage site" FT repeat_region 2037..2071 FT /rpt_type=INVERTED FT gene 2209..2520 FT /gene="mobB" FT CDS 2209..2520 FT /codon_start=1 FT /transl_table=11 FT /gene="mobB" FT /product="oriT recognition-like protein" FT /db_xref="GOA:Q840R4" FT /db_xref="InterPro:IPR002145" FT /db_xref="UniProtKB/TrEMBL:Q840R4" FT /protein_id="AAP04746.1" FT /translation="MPFTVQGLEPLDAVVNVRLTASEKARLREDADLAGLSVSELVRRR FT YFGRPIVAHADAVLLKELRRIGGLLKHVHNESGGAYSQQTAAVLVTLKAAIEGLSHDR" FT repeat_region 2242..2274 FT /rpt_type=INVERTED FT gene 2510..5179 FT /gene="mobA" FT CDS 2510..5179 FT /codon_start=1 FT /transl_table=11 FT /gene="mobA" FT /product="relaxase/primase-like fusion protein" FT /db_xref="InterPro:IPR005094" FT /db_xref="UniProtKB/TrEMBL:Q840R3" FT /protein_id="AAP04747.1" FT /translation="MIVKKVKSNRTKGKAASIRDLTNYIREPQNRNPNEKVLYANGRGF FT ISDTHAAQREEMVALAAEAVRSRNPVNHYILSWREGEQPSPEQVEEAVSIFLDELGLQE FT HQVIYALHKDTDNLHLHIAVNRVHPETLKCVEINKGFDLESAHRAIARIEHAQGWQREQ FT NGRYEVLENGELGREHLEPNKRRQPEQRKRDKENRTGEKSAERIAIEIGAPIIKQAQSW FT EQLHRELAAQGMRYEQKGSGALLWVGEVAVKASSADREASLGKLQKRLGAYEPAQAPSP FT VAQRKPEPLQPDRPEWEDFMAGRKMHYAEKNAAKLSMDQRQEQERKALQARQQEQRKVL FT LGGRWNGKGEALNALRGVLAAEQAAEKAALKERHQQERQQWRQQYRPYPDFEQWLRREH FT GAEQATRWRYREAEPQRIEGDSTEAPKPRDIRAYRAEIVGQEVRYTPKSGAGGGPGGVS FT FVDKGRIIEIHDWRNQDTTLAALQLSAQKWGKFTVTGNDEYKALCVKLAVEHGFQITNP FT ELQEVIRQERQRMRQERAQAMKSEQIKPFERYAEAVGAERYRVTSIKMRPDGSKQTFIL FT DKRDGITRGFTPEEIAQRTPEMQRLQRRGENLYYTPLSEGKHHILIDDMDREKLDRLIR FT DGYQPAVVLESSPGNYQAVITIPKLGTPFDKDVGNRLSDALNREYGDPKLSGAIHPHRA FT PGYENRKPKHRREDGSYPEVRLLKAERRECGKTLALSREIDAGYQRQAAEKALKTPVEQ FT FSEPNSTQTTPVSEKTATEAYWRHYRDVRKRQRGMLDLSRVDAMIAVRMRVTGFDQSAI FT EGAIYQCAPSIREQQESRDWTDYARRTARYAYSAAGDRQAADLGKYRQQWEKLEGRERQ FT QEQAKAREIERNGPSMSR" FT gene 4121..5179 FT /gene="repB" FT CDS 4121..5179 FT /codon_start=1 FT /transl_table=11 FT /gene="repB" FT /product="primase-like protein" FT /db_xref="UniProtKB/TrEMBL:Q93TU3" FT /protein_id="AAK56912.1" FT /translation="MKSEQIKPFERYAEAVGAERYRVTSIKMRPDGSKQTFILDKRDGI FT TRGFTPEEIAQRTPEMQRLQRRGENLYYTPLSEGKHHILIDDMDREKLDRLIRDGYQPA FT VVLESSPGNYQAVITIPKLGTPFDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR FT KPKHRREDGSYPEVRLLKAERRECGKTLALSREIDAGYQRQAAEKALKTPVEQFSEPNS FT TQTTPVSEKTATEAYWRHYRDVRKRQRGMLDLSRVDAMIAVRMRVTGFDQSAIEGAIYQ FT CAPSIREQQESRDWTDYARRTARYAYSAAGDRQAADLGKYRQQWEKLEGRERQQEQAKA FT REIERNGPSMSR" FT repeat_region 5195..5215 FT /rpt_type=INVERTED FT gene 5234..5458 FT /gene="pasA" FT CDS 5234..5458 FT /codon_start=1 FT /transl_table=11 FT /gene="pasA" FT /product="anti-toxin-like protein" FT /note="plasmid addiction system-like anti-toxin" FT /db_xref="GOA:Q93TU2" FT /db_xref="InterPro:IPR002145" FT /db_xref="UniProtKB/TrEMBL:Q93TU2" FT /protein_id="AAK56913.1" FT /translation="MLAIRLPTEVENRLEALAQATGRTKTFYAREAILEHLDDLEDLYL FT AEQRLIDIRAGRSRTYTLEEVERDLGLAD" FT gene 5442..5714 FT /gene="pasB" FT CDS 5442..5714 FT /codon_start=1 FT /transl_table=11 FT /gene="pasB" FT /product="toxin-like protein" FT /note="plasmid addiction system-like toxin; similar to FT pTF-FC2 plasmid PasB protein" FT /db_xref="InterPro:IPR007712" FT /db_xref="InterPro:IPR012753" FT /db_xref="UniProtKB/TrEMBL:Q93TU1" FT /protein_id="AAK56914.1" FT /translation="MAWRIEFDDKAKKDLAALDKSVAKRITAFLRERVAHLDDPRSIGE FT ALKGSKLGDFWKYRVGDWRIIASIEDEALRILVVRIGNRREVYRK" FT repeat_region 5717..5742 FT /rpt_type=INVERTED FT gene 5831..6706 FT /gene="repA" FT CDS 5831..6706 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="helicase-like protein" FT /db_xref="GOA:Q93TU0" FT /db_xref="HSSP:1G8Y" FT /db_xref="UniProtKB/TrEMBL:Q93TU0" FT /protein_id="AAK56915.1" FT /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFWALE FT AAMAVACREPGGDLLGLAPKYTGRVVYLAGEDPEPALIRRVHAIGQHLNQSAREAIAEN FT LTLEPIMGKRLNIMDEAHLHRIIEASSGARLIVLDTLSRIHNLDENSNGAMAHLVATLE FT HIAASTGASVLYLHHVSKGSAMAGQTDQQQAARGASALIDNARWCGYVAKMAEHEAARL FT SDRSFDRAPIGDRRGFFVRFGVSKQNYDATSLESWYQRVDGGVLMPVDLVEATSNGKVK FT NDGKGKRDEI" FT gene 6770..7681 FT /gene="repC" FT CDS 6770..7681 FT /codon_start=1 FT /transl_table=11 FT /gene="repC" FT /product="DNA-binding protein-like protein" FT /db_xref="GOA:Q93TT9" FT /db_xref="InterPro:IPR010522" FT /db_xref="UniProtKB/TrEMBL:Q93TT9" FT /protein_id="AAK56916.1" FT /translation="MKKGSAMTFDLTHARHDPAHCLAPGLFRSLKRGERKKLKLDVTYT FT YGKDRVEFSGPEPLGVDDMRVLQGLVAVAATCGSESRGIMLRPEPKSEAGHQLRLLLDL FT EWDAKEKDAMVAKGSFRQLASELGYATDSGGAFRVIRESIERLWKVSVIVQREGKRQGF FT RILSEYASDEDTGKLFVALNPRLAEAIIGARPHTRIEMAEVRALQTDPARLIHQRLCGW FT IDPGKSGRVELDTLCGYIWPDQASGSTMRMRRQTARNALPELTTVGWTVNEYAKGKWEI FT SRPKALALTLTSPCPNVDEPLP" FT rep_origin 7621..8098 FT /note="oriV region" FT repeat_region 7622..7733 FT /note="contains five iterons located within the oriV FT region; the central three 22-bp iterons are perfectly FT conserved while the flanking iterons have a single FT base-pair insertion or deletion; incompatibility FT determinant" FT repeat_region 7736..7754 FT /rpt_type=INVERTED FT misc_feature 7773..7813 FT /note="A+T rich region" FT misc_feature 7811..7826 FT /note="14/15bp region highly conserved in IncQ-like FT plasmids" FT repeat_region 7909..7935 FT /rpt_type=DIRECT FT repeat_region 7951..7979 FT /rpt_type=INVERTED FT repeat_region 8067..8086 FT /rpt_type=INVERTED FT gene 8100..8474 FT /gene="ORF13" FT CDS 8100..8474 FT /codon_start=1 FT /transl_table=11 FT /gene="ORF13" FT /product="unknown" FT /db_xref="InterPro:IPR021104" FT /db_xref="UniProtKB/TrEMBL:Q840R2" FT /protein_id="AAP04748.1" FT /translation="MEAGPITQQDVAAAADQLGGPANTNAAKIREILGRGSLATIQRHL FT QALRDAQRAPELPEAVQTIPTPPEAVSEASRGIWAAAWAMAEQRHAESLAHAPCWVITG FT ITFAYSGNGCQSLSPNTTLL" FT gene complement(8489..9058) FT /gene="ORF20.8" FT CDS complement(8489..9058) FT /codon_start=1 FT /transl_table=11 FT /gene="ORF20.8" FT /product="invertase/recombinase-like protein" FT /db_xref="GOA:Q840R1" FT /db_xref="HSSP:2RSL" FT /db_xref="InterPro:IPR006119" FT /db_xref="InterPro:IPR006120" FT /db_xref="InterPro:IPR009057" FT /db_xref="InterPro:IPR012287" FT /db_xref="UniProtKB/TrEMBL:Q840R1" FT /protein_id="AAP04749.1" FT /translation="MAQIGYARISTGDQNLDLQRDALNHAGCEHTFEDVASGAKAERTG FT LVQAMAFLRPGDVLTVWRLDRLARSLPHLIEVLNDLEQRGCGFRSLTESIDTTTPAGRL FT TFHLFGALGQFERDIIRERTLAGLAAAVARGRKGGRKPVMTPEKQQRAQQLIAQGLSVR FT EAATRLKISKTVLYRELQRKRGGNGS" FT gene complement(9342..9803) FT /gene="ORF17.4" FT CDS complement(9342..9803) FT /codon_start=1 FT /transl_table=11 FT /gene="ORF17.4" FT /product="hypothetical protein" FT /db_xref="GOA:Q840R0" FT /db_xref="InterPro:IPR003779" FT /db_xref="InterPro:IPR004675" FT /db_xref="UniProtKB/TrEMBL:Q840R0" FT /protein_id="AAP04750.1" FT /translation="MMEKRVDYGLVSPNGIRSLRDLETYLHGSNLEPALLELVKLRASI FT LNGCAFCIDMHSKEARARGEMEQRLYALAAWREAPFFSERESAALAWTDAITRIDEGVS FT DALYENVRRYFEEKELVDLTLAVVAINAWNRLAIAFRTPAGSYRPDLDR" FT repeat_region 9836..9862 FT /rpt_type=INVERTED FT gene complement(10521..11381) FT /gene="ORF33" FT CDS complement(10521..11381) FT /codon_start=1 FT /transl_table=11 FT /gene="ORF33" FT /product="hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:Q840Q9" FT /protein_id="AAP04751.1" FT /translation="MFFWKKKATRITPTLEKDLTQYDNAKESTTCSSDDIIIALQQLKD FT GDLKFFFEDGDDEDDITDYGDVDIEDEDNDSNAYFEQEQIKSIITSMAFSVAKLTKEYT FT INNNEYKQKISDIIKTHYSELSERFFEGVRVVKYDVLDGQEWLKEKEYFFENVIYNKFG FT EDIENTIKEIKSLFLNTSSLDFKEYAKGAFPDSKDNINSIKDDCVDKIKVLLAENEIFS FT KFISVSFYSGIKQLSLNMLYDMLENVLADEIKKHKVEITQFIEEPELTPLGYEKLCLEA FT LNEAG" FT repeat_region join(11916..11942,13192..13218) FT /rpt_type=INVERTED FT gene complement(11931..13145) FT /gene="transposase" FT CDS complement(11931..13145) FT /codon_start=1 FT /transl_table=11 FT /gene="transposase" FT /product="transposase-like protein of ISAtc1" FT /db_xref="GOA:Q840Q8" FT /db_xref="InterPro:IPR002560" FT /db_xref="UniProtKB/TrEMBL:Q840Q8" FT /protein_id="AAP04752.1" FT /translation="MVPEELFSLALGLVPPWLVDHVTFTVEEKRLDLHINFPKGSRFAC FT SVCGEECPVHDTRDHTWRHMDFFQHEAYLHARVPRVKCQEHGVHQISVPWAREGSRFTL FT LFEALIMTLVREMPVLTAARMVGETDKLLWRVIDHYVPEARAAVDMANVHAVGVDETSS FT RHGHDYITLFVDLNARRLLFATPGKDAKTFEKFSGDLQAHGGSAEAITDVSMDLSPAFQ FT KGAAEHLPNAEITFDRFHLMKLVNEAVDDVRKGEVLTQPNLKKTRWLWLKNDCNLKMKQ FT KEKLQELLKDQNLKTAQAYQFRLTFQDIFTIKNRHQGATLLKAWLENARTSDLPPIVRV FT AYTLMNHWDGVLRWFESQVSNGILEGFNSLLQSAKAKARGYRTHKNFIAMAYLILGKLD FT LRLPT" FT repeat_region 13514..13538 FT /rpt_type=INVERTED FT gene complement(13595..13855) FT /gene="ORF9.5" FT CDS complement(13595..13855) FT /codon_start=1 FT /transl_table=11 FT /gene="ORF9.5" FT /product="hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:Q840Q7" FT /protein_id="AAP04753.1" FT /translation="MTISSAPTFWSTFGNREDCCSFLQDLLIAEGRFLISSLMLGISAS FT SLNYLDGELSATRPLKDLLGSDAHFAFFTLSRSFSPEHLLF" XX SQ Sequence 14155 BP; 3231 A; 3874 C; 4015 G; 3035 T; 0 other; ggatccttgt ggagaaattg tcagacaggg cagttgcaag atcgaggcgc acccactgtc 60 cgaaagtagg ccaagcgcct tctggacagg agcagagatc gcacctctgg ggcggggcgg 120 gccgcggggt gtgtctgcaa gggcgaagcc cgcgcagcgt agcgaagttt ttgcgggact 180 tgtccgccca atttgttcac tgatgctgct ttgtctctgc ctgctcgatg gctccgatat 240 ccaggcggcc atagtatacg gttttcccat cgtgtttctc gtatccaaag ttatttgcta 300 cgttggctgc cacatcaatg ggtacgcaag ggccagactg gatgccgtcg aacgcagggc 360 actcttggac aataccaccg atgcgcgttt ggatggccgc ttcctcggcc ttgagacgtt 420 gcagcttttg ttcgacctgc cggacttctt ttgagtgcca ccagatatcg acctctccgg 480 cagcgtatga cccaatcccc acgactacaa gcaccagccc ggcccagacg gatctcgacc 540 aaagcgtttt tcccaagtca ctgcggaagg catcgacgag ctgctgtcca gctttggaca 600 gcagttctga tgacgcggcg gagattcttc ccttggctgc ttcttcggcg ttactggcga 660 ttttatttgc cgtgttctcc atggccttgg gcagtttgct gaacgtccca tagtaggcat 720 ccaaggtgac caggatggga aacatggcat cgttctgcgg aatgtccagg gcatgcgcga 780 tggcggagac cctgttgatg tcttcgggtg tcggttcccg cccggtgatg agtccgatgg 840 ctttcttcag atcactcata agccgccttc aatgcggtct gtgcggcctc tcgatagcgg 900 cgcagtgcgg agcgttccga gatggagagc ttggtatcgg cgctgcccag agaaagccgg 960 ttgtccacga gtttgtcggc caccagatcg tttagctctg gaaatatcac ggttccggaa 1020 acacggtttt tcagagtgct gttggcaaac cgggcaaatt tttcaggtgc cccgaaatac 1080 gtgttcaaca cggcataggt ggcatggtag ggcttggccg tttccagaaa atctttcaac 1140 aactccagcg agtcccgttg ccggttgatg ggccagagca tgatgagttc ccggttctgc 1200 tcctgtgcca catcgttcag gatgccgcca tactggatga gtgccgaagt ggcccgcgct 1260 gcggtattga ccacgatgct gtttttgggg tggttctcga tgatgttacc gagatggata 1320 tatccctctt cggtatccag gttgcagata tggcaaggca gcaggtcttt gagggccttg 1380 taggtatcgg gattgctgtc gtcactctcc accagaacac aggaatgact cccggagagc 1440 agtgcatcga cgagagccat tgtcacggtg gattttccga cgcccccctt gccaccaccg 1500 acgaccagta tcttgttgag agacttgctc ataagtcctc cgaatccggt ttgaccgtaa 1560 aggttccagc ctgcgggata ggtgctgtgt tcgcttggcc ggacgctgtt ggcgccggtg 1620 tggtggattt tttctttctg gtgactgttg atttgttctg cttctttcct gcaacgatct 1680 cagtgatggt tttcggtagt ataccgaaca cgtcatcatg cttcagggca tccgcaatct 1740 gtttgctggt gtagcctttc ttgatcaagg cttccaggtc actcttgatc gctctgatga 1800 catcggcttt cccgcctttg ccggtgactt ctccgggctg ctgggttttc tccagatttt 1860 ccagtgcagt ccttgctttt ttcgcgtatg cgtctatatc gtagagttgt ttcgagcttg 1920 ccatcctgtc gttcctcctg tgccggtttt cttaagcttt gcatagattc tacttcattg 1980 actttcgtaa agaaagcact acaatacgta taagctatat tttgaacagg atgggacttg 2040 tcatacacat gctacgcatg ttcccgccaa gccacgccca accattcggc gctacgcttc 2100 tcacggtccg gctcatcctg caaagggcaa gccccttgac cccggccagc gcggcgccat 2160 ggcttcttgc tgacccagac acaccccgcc gccgcgaaag gagacacgat gccatttacc 2220 gtgcaaggac tggaacctct ggatgcggtc gtcaatgtcc ggctaaccgc atcggagaaa 2280 gcgcgtctac gggaggatgc ggacctcgcc ggattgagtg tttccgagct ggtgcgccgc 2340 cgctactttg gcaggccgat tgtggctcat gcggatgcgg tcttgctcaa agagctacgc 2400 cgcatcgggg gattgctcaa gcatgtacac aatgaaagcg gtggagcgta cagccagcaa 2460 actgccgccg ttctggtcac tttgaaagcc gccatcgagg ggctgagtca tgatcgttaa 2520 aaaggtcaag agcaatcgaa caaagggcaa agcggcgagt atacgcgatc tgactaacta 2580 catccgggag ccgcagaacc ggaatccaaa tgagaaggta ctttacgcga acggacgggg 2640 tttcatcagc gacactcatg ccgcccagcg ggaggaaatg gtagccctgg cagcggaagc 2700 ggtacgcagc cgcaatccgg tcaatcacta catcctgagc tggcgggaag gggagcagcc 2760 tagcccggag caggtggaag aagcggtaag catctttctg gatgaacttg gcttgcagga 2820 acaccaggtt atctacgccc tgcacaagga cacggataac ctgcatctgc atatcgccgt 2880 caatcgcgta cacccagaaa cgctcaagtg tgtggagatc aacaagggct ttgaccttga 2940 atccgcgcat cgagctattg cccggattga acatgcacag ggatggcaac gggagcagaa 3000 cgggcgctat gaagtgttag agaatggcga gctaggacga gaacacctgg aaccaaacaa 3060 gcgacgccaa ccggagcagc gcaagcggga caaggagaac cgcaccgggg agaaatccgc 3120 ggaacgtatc gccattgaga tcggtgcgcc gatcatcaag caagcgcaaa gctgggaaca 3180 gttgcaccgg gaactggcag cacaagggat gcgctacgag cagaagggca gtggcgcatt 3240 gctttgggtc ggtgaggtag cggtcaaagc cagcagtgcc gaccgggaag cgagcctcgg 3300 caaactgcaa aagcggctgg gcgcctatga acccgcgcaa gcaccttcgc ctgtggcgca 3360 acggaagccg gaaccgcttc aacccgacag gccggagtgg gaggacttca tggctggacg 3420 caaaatgcac tacgcagaaa agaacgcggc caagctctcc atggaccagc ggcaggaaca 3480 ggaacgcaag gcattgcaag cgcggcagca ggaacaacgc aaggtactcc tgggcggacg 3540 atggaatggc aagggagaag cgctgaacgc gctgcgcggc gtgcttgccg cagaacaggc 3600 cgcggagaaa gccgccctga aagaacgtca ccagcaggaa cggcaacagt ggcggcaaca 3660 gtaccgcccg tatcccgact tcgagcagtg gttacgacgc gagcacgggg cagaacaggc 3720 gactaggtgg cgctaccggg aggcggaacc gcaacgcatc gagggcgact cgaccgaagc 3780 gccgaaacct cgggacattc gcgcctaccg ggcggagatc gtgggacagg aggtccgtta 3840 cacccccaag agtggcgcgg gcggcgggcc ggggggtgtg tcttttgtgg acaagggcag 3900 gatcatcgaa atccacgatt ggcggaacca ggacaccact cttgcggcgc tccagctctc 3960 ggcgcagaaa tggggcaagt tcaccgtgac cgggaacgac gaatacaaag cgctgtgcgt 4020 gaagctggcg gtggagcacg gtttccagat cacgaacccg gagcttcagg aggtcattcg 4080 gcaggaacgg caacggatgc ggcaggagag ggcacaggcg atgaaatcgg agcagatcaa 4140 gccgttcgag cgatacgccg aagcggttgg cgccgagcgc taccgggtga caagcatcaa 4200 aatgcggcca gatggcagca agcaaacctt cattctcgat aagcgggacg gtatcacgcg 4260 cggcttcacg ccggaagaaa tcgcccaaag gacgcccgaa atgcagcgtt tacagcgccg 4320 gggcgaaaac ctgtattaca cgccgctctc cgaagggaaa caccatatcc tgatcgacga 4380 catggaccgt gagaaactgg accggctgat tcgtgatggc tatcagcccg ccgtcgtgct 4440 ggaatccagc cccggcaact atcaggcggt catcaccatt ccgaagctgg ggaccccctt 4500 cgacaaggac gtggggaacc gcctgagcga tgcgctcaac cgcgaatacg gcgaccccaa 4560 gctgtctggt gccatccatc cgcaccgcgc tcccggctac gagaaccgca agcccaagca 4620 ccggcgggaa gatggcagct atcccgaagt gcgtttgctc aaggccgagc ggcgcgagtg 4680 cggcaagacg ctggcgcttt cccgcgagat cgacgccgga tatcagcggc aggccgccga 4740 gaaggccctg aaaacgcccg tagagcaatt ttcggagcca aacagtaccc agaccacccc 4800 tgtctctgaa aaaaccgcca cagaggctta ttggcggcat taccgcgatg ttcgcaagcg 4860 ccaacgcgga atgctggacc tgtcccgcgt ggacgccatg attgccgtgc ggatgcgcgt 4920 taccggcttt gaccagtcgg ctattgaggg cgccatctac cagtgtgccc catcgatccg 4980 ggagcagcag gaaagccggg actggaccga ctacgcccgc cgcacggccc gctatgccta 5040 cagcgccgca ggtgaccggc aggccgccga cctgggtaag taccgccagc agtgggagaa 5100 gctggagggg cgcgagcggc agcaggagca ggccaaggcg cgggagattg agcgcaacgg 5160 gccgagtatg agtcgttagt attcttcaca gcggcatgat atacttgtat atcattttga 5220 gcaggagcta aacatgcttg ccatccgatt acccaccgaa gtggaaaatc gccttgaggc 5280 cttggcgcag gccacaggac gcacgaaaac cttttacgcc cgcgaagcga tccttgagca 5340 cctggacgac ctcgaagatt tgtaccttgc agagcaacgc ctgatcgaca ttcgcgcagg 5400 ccgaagccgt acctacacgc tagaggaagt ggagcgcgat cttggcttgg cggattgagt 5460 ttgatgacaa ggccaagaaa gacttggctg cgctggataa gagcgttgcc aaacgcatca 5520 cggcttttct gcgagagcgc gtcgcccatc tagacgaccc gcgcagcatt ggcgaagccc 5580 tcaaaggctc caagctggga gacttctgga agtaccgcgt aggggattgg cgaatcattg 5640 ccagcattga ggatgaagcc ttgcgtattc ttgtcgtgcg tattggtaac cgccgagagg 5700 tataccgaaa gtaaacaacc ccggcatgaa gggccggggt ttcccgcgca accggatgaa 5760 aggacggcgc gagccggagt gtcagtcccc ggcagcgcct aaccacgcca gttctggaaa 5820 ggagaacagc atggctttgg acattatggc ggcattcacc aacgaaccgc cagaactgga 5880 tttcatctgg ccgggattcc tggccgggac cgtgggcgca ctggttgcac ctggcgcgac 5940 cggcaagagc ttctgggcct tggaagcggc gatggccgtc gcgtgccggg agcctggcgg 6000 agacctgctg gggcttgcgc cgaagtacac cgggcgcgtt gtctatctgg ccggagaaga 6060 tccagaacca gccttaatcc ggcgcgttca tgccataggc cagcatctca accagtcggc 6120 ccgcgaagcc attgctgaga atctgaccct tgagccgatc atgggcaagc ggctgaacat 6180 catggacgag gcccacctgc atcgcatcat cgaggccagc tccggggcac ggctgatcgt 6240 gctggacacc ctgagccgta ttcacaatct ggacgagaac agcaacggtg ccatggcgca 6300 tctggtggca accctggagc atattgccgc cagtaccggc gcatcggtgc tgtacctgca 6360 tcatgtcagc aaaggcagcg ccatggccgg gcagacggat cagcagcaag cggcgcgggg 6420 ggcatcagcc ctgatcgaca atgcccgttg gtgcggctac gttgccaaaa tggcagagca 6480 tgaggccgcg cgactgagcg accgtagctt tgaccgtgcg cccattggcg accggcgcgg 6540 cttcttcgtg cgcttcggtg tgagcaaaca gaactacgac gcaacatcgc tcgaaagctg 6600 gtatcagcga gtggatggcg gggtgttgat gcccgtcgac ctggtggaag caacgagcaa 6660 tgggaaggta aaaaacgatg gcaaaggaaa gcgcgatgag atctgaccct gtgctatcgc 6720 accccctgcc gatccccttt ttgtgtggct atacccaggg aggcaagcca tgaagaaggg 6780 aagcgcgatg accttcgacc tcacccacgc aagacacgat ccggctcact gtctggctcc 6840 tggtcttttt cgcagcctca agcgtggcga gcgaaaaaaa ctcaagttgg acgtgaccta 6900 cacttacggt aaggatcgtg ttgagtttag cggtccagag ccgcttggtg ttgatgatat 6960 gcgtgtcctg caaggtctcg ttgccgtcgc cgccacctgt gggtcggaaa gtcggggaat 7020 catgctgcgc cctgagccaa aaagtgaagc gggacatcag ttgaggctgt tgcttgattt 7080 agaatgggac gccaaggaaa aagatgccat ggtcgctaaa ggcagcttcc ggcaacttgc 7140 aagcgagctt ggatatgcta cagacagtgg cggcgcgttt cgtgtcattc gagaaagtat 7200 cgaacgacta tggaaggtct ccgtgatcgt tcagcgcgag gggaaacgac agggattccg 7260 catcctctcc gaatatgcca gcgacgaaga caccgggaag ctatttgttg ccttgaaccc 7320 cagactggct gaggcaatca tcggagcacg tccacacacc cgtatcgaga tggcagaggt 7380 gcgagcactg cagactgacc ctgcccgtct cattcatcag cggctatgcg gttggatcga 7440 ccccggcaag tccgggcgcg tcgagctgga cacgctttgc ggttatatct ggcccgatca 7500 ggccagcggg tcaaccatgc gtatgcgccg acagactgct cgtaatgcgc tgcccgagct 7560 taccactgta ggctggacgg tgaacgaata cgccaaggga aaatgggaaa tcagcagacc 7620 gaaggccctt gccctaacgt tgacgagccc ctgccctaac gttgacgagc ccctgcccta 7680 acgttgacga gcccctgccc taacgttgac gagcccctgc cctaacgttg acaccacaaa 7740 acacgctaag ccagcattga cggggctttg cggaagattt gaaaaatcga tccaagatat 7800 tctccaagaa tatccactat gcgcggcctt cagccgccgc ttgatgcgtc gtcagaagcc 7860 ttgcacttcg gccttcggcc tgcgcgggct acgcccttgc agacacaccc cgccgcccgc 7920 ccgcccgccg cccgcttgta cgttctccaa ggtggataac gtgacttacg ttatccacca 7980 accatgccgc cgatagtgag cacctactgg gtagacgagc ggctgatggt gtaaataacc 8040 ttgacaaata gatgtaaatt tactatatta catttacaat gtaattacag gagagtgcca 8100 tggaagcagg accgatcaca caacaagacg ttgccgccgc cgctgaccag cttggcggcc 8160 ccgccaacac caatgccgcg aaaatccggg agattctggg ccgggggagt ctcgccacca 8220 ttcaacgcca tttgcaggcc cttagagacg ctcagagggc tccagagcta ccagaggccg 8280 tccagacgat tccaacgcct cctgaggccg tttctgaggc ttccagaggc atttgggcgg 8340 cggcctgggc aatggcggag caacgccacg cggaaagtct ggcgcatgcc ccctgctggg 8400 ttataacagg tattactttt gcgtactctg gcaacggatg ccagtctcta tctcccaata 8460 caaccctgct ttagggcgaa aatccatgct atgaaccatt cccgccacgc ttacgctgca 8520 actcgcgata gaggacggtt ttactgatct tgaggcgggt ggcggcctcg cggacgctga 8580 ggccctgggc gatcaactgc tgggcgcgtt gctgcttctc tggggtcatg acgggtttgc 8640 gtcccccttt gcggccacgg gcgactgcag cggcgaggcc tgccaaggtg cgctcccgga 8700 taatgtcacg ctcgaactgt ccgagcgccc caaagagatg gaaggtcagt ctcccggctg 8760 gggtggtggt gtcaatggat tcggtcaggg accgaaaacc gcacccgcgt tgttccaggt 8820 cgttcaggac ctcgatgagg tgggggagag aacgggccag gcgatccagt cgccagacgg 8880 tgagcacgtc gccagggcgg aggaaggcca tggcctgcac caatccggtg cgttccgcct 8940 tggccccgct ggcgacatcc tcaaaggtgt gttcgcaccc ggcatggttc agggcatccc 9000 gctgtaggtc gaggttctgg tctccggtgg atatccgcgc ataaccgatc tgtgccatgg 9060 gtgtcgcctg tgtgcgtcaa gtcgtcccac aatcatagcg tccgtctatc cgttgaaaca 9120 gggggttgtg ggacgctttt gggggtgtcc gtcagaggat cgggatcacc cttcgtctgc 9180 cagagccgga gtttcagcca tccgtcccca tagcgccaga cccgccgata ggtttcgcgc 9240 tcccagtcat ccatccggtt gtgggcttga cggcgcagcg cccagaccgt gagatccagg 9300 cgaaagggcg gtcgcgggct gaggcggaag tagtatccca tctatcggtc cagatccggc 9360 cgataactgc cggcgggggt acggaaggca atggctaggc ggttccaggc gttgatggcc 9420 acgacggcca aggtcagatc caccagttcc ttctcctcga aataacggcg gacgttttca 9480 tagagcgcat ccgagacgcc ttcatcgatg cgggtgatag catcggtcca cgccagcgcc 9540 gcgctttccc gctcgctgaa aaatggcgct tcccgccaag cggcgagggc ataaagacgc 9600 tgctccatct cgccccgggc gcgggcttcc ttgctgtgca tgtcgatgca aaaggcgcag 9660 ccgttgagga tggatgcgcg cagcttcacc agttccagca gcgctggttc caagttgctt 9720 ccatggagat aagtctccag atcccgcagg ctgcggatgc cgttggggga taccaagcca 9780 tagtcgacgc gtttttccat catggactcc tgtgggaacc ttcttggagc ggaaggggat 9840 ctggggccgg ataccagatc ccgccatcgg gacgccgccg cgcccaaggg ctcaaacgat 9900 gcctctggtc atgccctggc gtcagcaggt gatgccacgg taaaaccctg ggcggtaagg 9960 acgtcagcaa tgagaaagcg atgacagcgc cacgggaggg tgctcggcgc acaggaggac 10020 cgtcctgcgc ctacgcgacc aaggtgctcc aaggcctcca atcctttctg gaaaggcgcc 10080 gtctgcgcaa gcgaccatat cggcagatac tgccaggata gagccagaag actgatctac 10140 cggcacagcg cgaccattct aatcacggat gttcagaggt tccaaaggaa tatcatgagc 10200 gccacatatc acgatccacg atacgctagg caggatggct agacaggccg cctccgcaga 10260 cggtatctga tcctgagcga gcgttgccca tagtaagctt gcggggtatc ctctaactga 10320 agaacgacgg cttgagactg gaagcagacg ttcggttggt gcgccaagaa aggcgcatcg 10380 aatctcctgc cagtccaagg ttagtcagca ttgggcatga tctgtaagct cgcttgctat 10440 cacacctcct aataatcaac tatgattgac gctggcggta caatccgatg aggaatggca 10500 acgtgaacaa gcaagaattc ctatcctgct tcgttaaggg cctctaaaca tagcttctca 10560 tatcctagtg gtgtcaattc aggttcttct ataaactgtg taatttcaac cttatgtttt 10620 tttatttcat ctgccaatac attttcaagc atatcgtaca gcatattaag acttagttgt 10680 ttaatcccag aatagaacga taccgaaatg aattttgaaa atatctcatt ttcagcaaga 10740 agcactttta tcttatccac acaatcatct tttatactat ttatgttatc tttgctgtct 10800 ggaaatgctc ccttagcata ttccttaaaa tccaatgagc ttgtatttag gaacaacgat 10860 tttatttctt ttattgtgtt ttctatatcc tctccgaatt tattgtatat cacattttca 10920 aaaaaatatt ccttctcttt taaccattcc tgaccgtcaa gaacatcata tttgacaacc 10980 cttaccccct cgaaaaatct ttctgacagc tcgctgtaat gtgtcttgat gatatctgaa 11040 attttttgtt tgtactcgtt attgtttatg gtgtattctt tagtcagttt tgcaacagaa 11100 aacgccatag atgtgattat actctttatt tgttcttgct caaaataagc atttgaatca 11160 ttatcttcat cctctatatc aacatcgcca tagtcagtta tgtcatcctc gtcatcaccg 11220 tcttcaaaaa agaactttaa atcgccatcc tttagttgtt gaagggctat tattatatcg 11280 tcagatgagc atgtcgtgct ttccttggcg ttatcatact gcgtcaagtc tttctctagt 11340 gttggagtaa tacgtgttgc cttttttttc cagaaaaaca tataaaaggc tcctagattt 11400 tttattgaga gcgataccag acaaaatgca tcctttggtc cggttccggc cggggaccgg 11460 agcagaaccc attgattcca tgagagccga gagccgcaga aggccggcaa acatctgtcc 11520 ttacggtgaa catggtcatt tccacaccga atccccgaat tcaccgtata gaagacctga 11580 tggcacccta ggatatgaga gctatgttag tgccttaacg aagcaggatg gattctgaac 11640 taacaaaatc ggaccggcga ccaaggtgct gatataattg caaaaaagaa tgataattct 11700 ttggtggtgc agtgcaaata ttactccggt gggcgttgaa ccgaaagcag tccaggaagt 11760 tatcgctgcg cgtacatatt attgtgctga ttatgccacc gttgtatcaa acacatactt 11820 cacacctggc gcgaaagatt tagcaaaaag aggcgatgtt ttactattgg acgaaaacaa 11880 gttgaagaat attgactttt ttttaaaaaa cagttaggct cttcgacgtt tcatgtgggt 11940 agcctcagat ccagcttgcc caggatgagg taagccatgg cgatgaagtt cttgtgggtg 12000 cggtagccac gggctttcgc cttggcggac tggaggaggc tgttgaagcc ctccaggatg 12060 ccattgctga cttggctctc gaaccagcgg aggaccccgt cccaatggtt catgagggtg 12120 taggcgaccc tgacgatagg cggcagatcg ctggttctgg cgttttccaa ccaggctttc 12180 aagagggtag ccccctggtg gcgattcttg atcgtgaaga tgtcctgaaa ggtcaggcgg 12240 aactggtagg cctgcgccgt cttgaggttc tggtctttga gcaattcctg cagcttttct 12300 ttctgcttca ttttgaggtt gcaatcgttc ttgagccaaa gccagcgggt ctttttgaga 12360 tttggctggg tgaggacttc ccccttgcgc acgtcgtcta cggcctcgtt gacgagcttc 12420 atgaggtgaa aacgatcgaa agtgatctcc gcattgggca ggtgctcggc agcccctttc 12480 tggaaggccg gcgagaggtc catgctcaca tcggtgatcg cttccgcgct accaccatgg 12540 gcctgaagat ctccggagaa tttctcaaag gtcttggcat ccttgccggg agtagcgaac 12600 aagagtcgcc gggcattcag atccacgaag agcgtgatgt agtcatgtcc atgccgactg 12660 ctggtttcat cgacgccgac ggcatggaca ttggccatat ccaccgcagc acgagcttcg 12720 ggcacataat ggtcaatcac tcgccacagg agcttgtcgg tctcaccgac catgcgagct 12780 gccgtcaata ccggcatctc ccgcaccagg gtcatgatca gcgcttcaaa gagcagggtg 12840 aaacgcgagc cttcccgcgc ccagggaaca gatatctgat gcactccatg ctcctggcac 12900 ttcacacgag gtacacgggc atggagatag gcttcatgct ggaagaaatc catgtgccgc 12960 caggtatggt cacgggtatc atgtaccgga cactcctcac cacagacgga gcaagcaaag 13020 cgactacctt tgggaaagtt gatgtgcaga tccaggcgtt tctcctccac cgtgaaagtc 13080 acatgatcca ccaaccacgg cggaaccaat cctaacgcga gagaaaacag ctcttcaggg 13140 accatcagct aactcctatt cagggcacga ccgctccaca gcattctacc ccctacccac 13200 tcaatctgac gaagagccat aaggaaggat ctcgatatca taagtttcca gtgatgtgaa 13260 tcgttctttg gtgccatcca actcccatag aatctggctg tctctaagcg cacgtaatcc 13320 atacagccag aaatcaccgg gcatgtcgct cgggtctaac ctaaactgca tgctcccggc 13380 atggatgctg aagacaatct gatgcctttc cccatctagt ggatatggtt tactattact 13440 tctgtgctca tcgaaaccat ctttcttttt ggaccagtat aaggttgtgt ttgcgctcaa 13500 tgtcctatta tttgtaatca ctgcgggagc agtgattagg ctggcctttt cctgctgtac 13560 cgcctccaat ataaactggt agacggaccc atcttcagaa cagaagatgt tctggactaa 13620 agcttctgga caaggtaaaa aaagcgaaat gtgcgtctga tccaagcaag tcctttaggg 13680 gtcgggtagc ggaaagttct ccatccaaat agttcaagga tgacgccgat atgcccaaca 13740 ttagggatga aatcaagaat ctaccttcag ctattagtag gtcttgcagg aagctgcaac 13800 aatcttcccg gttcccgaag gtgctccaga atgtcggcgc agacgatata gtcataacgc 13860 agcttctgaa gattgcgcgg aagatgctcc cgctccagat cccactccca taagtgacga 13920 tagaagggtc gtgccgcttc tagttcaatg tgattggcat cgataccatc caccacgcag 13980 tgcagacttt cactcaggta cttccccaac gtgcctacac cacaaccaac gtccaggaca 14040 aggctgccgg gctgaatgcg tcgggcaatc tttgcaagac tatcttggcc ctgaggatct 14100 atcgggggac gattataaac atgcggggta tgtgggactg attcatacgg gatcc 14155 //