ID AF012911; SV 1; circular; genomic DNA; STD; UNC; 4273 BP. XX AC AF012911; XX DT 31-JAN-1998 (Rel. 54, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 5) XX DE Plasmid pETHIS-1, complete sequence. XX KW . XX OS Plasmid pETHIS-1 OC other sequences; plasmids. OG Plasmid pETHIS-1 XX RN [1] RP 1-4273 RX PUBMED; 10463177. RA Schaller A., Kuhn R., Kuhnert P., Nicolet J., Anderson T.J., MacInnes J.I., RA Segers R.P., Frey J.; RT "Characterization of apxIVA, a new RTX determinant of Actinobacillus RT pleuropneumoniae"; RL Microbiology (Reading, Engl.) 145(Pt 8):2105-2116(1999). XX RN [2] RP 1-4273 RA Kuhn R., Anderson T.J., MacInnes J.I., Segers R.P.A.M., Nicolet J., RA Frey J.; RT ; RL Submitted (08-JUL-1997) to the INSDC. RL Institute for Veterinary Bacteriology, University of Berne, Laenggasstrasse RL 122, Berne CH-3012, Switzerland XX DR RFAM; RF00106; RNAI. XX FH Key Location/Qualifiers FH FT source 1..4273 FT /organism="Plasmid pETHIS-1" FT /plasmid="pETHIS-1" FT /host="Escherichia coli" FT /mol_type="genomic DNA" FT /note="derived from pET14b, for production of fusion FT proteins with an N-terminal histidine hexamer and/or FT C-terminal histidine decamer tail in Escherichia coli" FT /db_xref="taxon:64374" FT promoter 36..52 FT /note="T7 RNA polymerase promoter" FT gene 93..266 FT /gene="his6-mcs-his10" FT RBS 93..97 FT /gene="his6-mcs-his10" FT CDS 105..266 FT /codon_start=1 FT /transl_table=11 FT /gene="his6-mcs-his10" FT /product="histidine hexamer-multiple cloning site-histidine FT decamer" FT /db_xref="UniProtKB/TrEMBL:O55244" FT /protein_id="AAB97883.1" FT /translation="MGSSHHHHHHSSGLVPRGSHMISNSSLVPLALVSSPVSSGRGSHH FT HHHHHHHH" FT misc_feature 162..234 FT /gene="his6-mcs-his10" FT /note="multiple cloning site" FT gene 498..1358 FT /gene="bla" FT CDS 498..1358 FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta-lactamase" FT /db_xref="GOA:Q7BVP8" FT /db_xref="HSSP:1ALQ" FT /db_xref="InterPro:IPR000871" FT /db_xref="InterPro:IPR001466" FT /db_xref="InterPro:IPR012338" FT /db_xref="InterPro:IPR023650" FT /db_xref="UniProtKB/TrEMBL:Q7BVP8" FT /protein_id="AAB97881.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT rep_origin 1570..2800 FT /note="origin of replication from pMB1" FT gene complement(2547..2738) FT /gene="rop" FT CDS complement(2547..2738) FT /codon_start=1 FT /transl_table=11 FT /gene="rop" FT /product="Rop" FT /note="repressor of primer" FT /db_xref="HSSP:1B6Q" FT /db_xref="InterPro:IPR000769" FT /db_xref="UniProtKB/TrEMBL:Q7BVP7" FT /protein_id="AAB97882.1" FT /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA FT DELYRSCLARFGDDGENL" XX SQ Sequence 4273 BP; 1017 A; 1125 C; 1158 G; 973 T; 0 other; gatcgagatc tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt 60 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc 120 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatgata tcgaattcaa 180 gcttggtacc gctagcacta gtgagctcac cggtctcgag cggccgcgga tcccaccatc 240 accatcacca tcaccatcac cattaatcga tgataagctg tcaaacatga gcttgaagac 300 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 360 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 420 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 480 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 540 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 600 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 660 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 720 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact 780 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 840 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 900 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 960 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 1020 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg 1080 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 1140 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 1200 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 1260 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 1320 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 1380 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 1440 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 1500 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 1560 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 1620 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 1680 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 1740 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 1800 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 1860 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 1920 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 1980 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 2040 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 2100 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 2160 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 2220 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 2280 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 2340 tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag 2400 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 2460 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 2520 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 2580 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt 2640 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag 2700 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga 2760 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt 2820 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg 2880 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat 2940 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg 3000 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat 3060 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc 3120 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac 3180 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg 3240 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc 3300 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg 3360 tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg 3420 ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg 3480 ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa 3540 gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca 3600 tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg 3660 ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc 3720 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt 3780 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc 3840 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt 3900 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga 3960 aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact 4020 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga 4080 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac 4140 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga 4200 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc 4260 gtccggcgta gag 4273 //