ID AB243053; SV 1; circular; genomic DNA; STD; PRO; 6610 BP. XX AC AB243053; XX DT 21-SEP-2006 (Rel. 89, Created) DT 21-SEP-2006 (Rel. 89, Last updated, Version 1) XX DE Bacillus subtilis plasmid pLS30 DNA, complete sequence. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. OG Plasmid pLS30 XX RN [1] RX DOI; 10.1093/jb/mvj058. RX PUBMED; 16567421. RA Sakaya N., Kaneko S., Matsunaga S., Itaya M.; RT "Experimental basis for a stable plasmid, pLS30, to shuttle between RT Bacillus subtilis species by conjugational transfer"; RL J. Biochem. 139(3):557-561(2006). XX RN [2] RP 1-6610 RA Itaya M., Sakaya N., Kaneko S.; RT ; RL Submitted (24-NOV-2005) to the INSDC. RL Mitsuhiro Itaya, Institute for Advanced Biosciences Keio University; 403-1 RL Nipponkoku, Daihouji, Tsuruoka, Yamagata 997-0017, Japan RL (E-mail:mita2001@sfc.keio.ac.jp, Tel:81-235-29-0526, Fax:81-235-29-0529) XX DR RFAM; RF01691; Bacillus-plasmid. XX FH Key Location/Qualifiers FH FT source 1..6610 FT /organism="Bacillus subtilis" FT /plasmid="pLS30" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT gene 263..1186 FT /gene="rep" FT CDS 263..1186 FT /codon_start=1 FT /transl_table=11 FT /gene="rep" FT /product="replication protein" FT /db_xref="GOA:Q0EDI3" FT /db_xref="InterPro:IPR000989" FT /db_xref="UniProtKB/TrEMBL:Q0EDI3" FT /protein_id="BAE93219.1" FT /translation="MAEHYEALESKIGAPYYGKKAEKLISCAEYLSFKRDPETGKLKLY FT QAHFCKVRLCPMCAWRRSLKIAYHNKLIVEEANRQYGCGWIFLTLTVRNVKGERLKPQI FT SEMMEGFRKLFQYKKVKTSVLGFFRALEITKNHEEDTYHPHFHVLIPVRKNYFGKNYIK FT QAEWTSLWKKAMKLDYTPIVDIRRVKGKAKIDAELIENDVREAMMEQKAVLEISKYPVK FT DTDVVRGNKVTEDNLNTVLYLDDALAARRLIGYGGILKEIHKELNLGDAEDGDLVKIEE FT EDDEVANGAFEVMAYWHPGIKNYIIK" FT CDS complement(1216..1719) FT /codon_start=1 FT /transl_table=11 FT /product="putative transmembrane protein" FT /db_xref="GOA:Q0EDI2" FT /db_xref="UniProtKB/TrEMBL:Q0EDI2" FT /protein_id="BAE93220.1" FT /translation="MSLVLLIPTNSILAAQQNNNEQSKDITTPIVPTTFSQNGITCSSN FT QTNQNITIQQLPGDYLNSCKIVATKKYDNVKYTDWENKIIPAIAGGVVGVIGNYIAQGP FT LKAFVFGAMTTVYLNYPKSKPVYVTIQHRQCKDSLGYHRQLIIKSYKDKKRTKHISTQY FT KWVS" FT gene 2348..2785 FT /gene="hsp" FT CDS 2348..2785 FT /codon_start=1 FT /transl_table=11 FT /gene="hsp" FT /product="stress response homolog Hsp" FT /db_xref="HSSP:1GME" FT /db_xref="InterPro:IPR002068" FT /db_xref="InterPro:IPR008978" FT /db_xref="UniProtKB/TrEMBL:Q9X3Z5" FT /protein_id="BAE93221.1" FT /translation="MSLVPYDPFRQLSNMRREFDRFFSELPISFDNEHGIGGIRVDVHE FT TENEVVATCDLPGLEKKEDVDIDIQNNRLSISGSIKRTNEIKEENMLKKERYTGRFQRM FT ITLPSPVSHDGVKATYKNGILEITMPKVAKDVKKKIDVSFQ" FT gene 3630..5078 FT /gene="mob" FT CDS 3630..5078 FT /codon_start=1 FT /transl_table=11 FT /gene="mob" FT /product="mobilization protein" FT /db_xref="GOA:Q5MCL2" FT /db_xref="InterPro:IPR001668" FT /db_xref="UniProtKB/TrEMBL:Q5MCL2" FT /protein_id="BAE93222.1" FT /translation="MANYAVIRMEKYKKDRLNGTQKHNQREFQKSKNENIDRERTHLNY FT DLVNEKPISYSKAIHEKIEGRVKRKVRADAVLVSEFLITASPDYMNGLSDEEQRRYFET FT AVDHLKEKYSAENMLYATVHMDEATPHMHVGIVPITEDGRLSAKDFFNGKLKMKAIQDD FT FHRHMVENGFDLVRGEPSEKKHENVHQYKINQRQAELERLNAEIALKEKQREELEKQNK FT AVQAVIEVKKESLTAKAEELKMPTIEHEKAWLKKDKVIVPERELHALYAYAEQKTKTAA FT ELAGQLKSETQEKERWQSIARQEADRADEKDQRLQELQSRIHSEVEASKKEMRRKLAKE FT FTEEQRQDLRQEVKEELTTLRTENEELSAENKVLIIQRNSEAAESLKLKQELDKRNGQY FT AEVLSFAKKQNQTLEKVAGENKALKKENKTLKERVAVLEQWKDKMVQWAKEKLPKMRKL FT AASFFRTAGMPREANKYKDNELER" FT CDS complement(5089..5568) FT /codon_start=1 FT /transl_table=11 FT /product="putative DNA-binding protein" FT /db_xref="GOA:Q45449" FT /db_xref="InterPro:IPR009061" FT /db_xref="UniProtKB/TrEMBL:Q45449" FT /protein_id="BAE93223.1" FT /translation="MDMGIKLSSKDVAKHLGIEPVTVRKYASMLEEQGYSFNKDSKGWR FT LYTEDDVKTLEYLCTMTKINGHSLDETVKHIASLYRSKLTISDTATSLQDNPLVEFMKR FT QEEFNRHMAERMERFEKRQQERHETLTAALRESLETQKMIAATKQKKWWKFWKGN" XX SQ Sequence 6610 BP; 2119 A; 1069 C; 1529 G; 1893 T; 0 other; ttgatactat atagaaacaa catcattttt caaaatcagt tcaaagtctt gtgtgtcaag 60 ggttagatcg gtttttgaca ggtaaaaact ccttctgcta ttattaaggt gtcgaatcaa 120 aataatagaa tgctagagaa ctagctcaga aggagttttt ttgttgattt attcatctga 180 aaacgattat agcatcctcg aagataaaac cgcaacaggt aaaaagcggg attggaaggg 240 gaaaaagaga cgtacgaacc tcatggcgga gcattacgaa gcattagaga gtaagattgg 300 ggcaccttac tatggcaaaa aggctgaaaa actaattagt tgtgcagagt atctttcgtt 360 taagagagac ccggagacgg gcaagttaaa actgtatcaa gcccattttt gtaaagtgag 420 gttatgcccg atgtgtgcgt ggcgcaggtc gttaaaaatt gcttatcaca ataagttgat 480 cgtagaggaa gccaatcggc agtacggctg cggatggatt tttctcacgc tgacggttcg 540 gaatgtaaag ggagaacggc tgaagccaca aatttctgag atgatggaag gctttaggaa 600 actgttccag tacaaaaaag taaaaacttc ggttcttgga tttttcagag ctttagagat 660 taccaaaaat catgaagaag atacatacca tcctcatttt catgtgttga taccagtaag 720 gaaaaattat tttgggaaaa actatattaa gcaggcggag tggacgagcc tttggaaaaa 780 ggcgatgaaa ttggattaca ctccgattgt tgatattcgt cgagtgaaag gtaaagccaa 840 gattgacgct gaactgattg aaaacgatgt gcgggaagca atgatggagc aaaaagctgt 900 tctcgaaatt tctaaatatc cggttaagga tacggatgtt gtgcgcggca ataaggtgac 960 tgaagacaat ctgaacacgg tgctttactt ggatgatgcg ttggcagctc gaaggttaat 1020 tggatacggt ggcattttga aggagataca taaagagctg aatcttggtg atgcggagga 1080 cggcgatctg gtcaagattg aggaagaaga tgacgaggtt gcaaatggtg catttgaggt 1140 tatggcttat tggcatcctg gcattaaaaa ttacataatc aaataaaaaa agcagacctt 1200 taaggcctgc tttttttaac taacccattt gtattgtgtt gaaatatgtt ttgttcgttt 1260 tttatctttg tagcttttta tgattaattg tctatgataa cctagggaat ctttacattg 1320 tctgtgttga atagttacat aaactggttt gctcttagga tagtttaaat atactgttgt 1380 cattgctccg aaaacaaaag cttttaatgg tccttgagcg atatagtttc ctataactcc 1440 aactacgcca cctgcaattg ctggaataat tttattttcc caatctgtat atttcacatt 1500 atcatacttt ttggtagcga caattttgca tgaatttaaa tagtcaccag gaagctgttg 1560 gatagttata ttttgattag tttggtttga agaacaggta ataccatttt gagaaaaagt 1620 ggttggaact attggtgttg ttatatcttt actctgttca ttgttatttt gttgagcagc 1680 aagtatactg ttcgtaggaa tcaataaaac taaagacatt actaaagcta atacaatttt 1740 tatgctacca ttcaattaaa aaacatccct tctttggtta tatgggcccg tatataatat 1800 acctcttatt gggatttttt gtttaaattt tgaattaaat taagagaaaa ttaagaaggg 1860 tatgaatttc acagtgaaat tactaatgag ttttgtgttt ggagctttag gggcattatt 1920 aatggtaagt gtgagagaac atttttcttt tactgtgaca ttaatagttt tagttgttgt 1980 tatcatcgtt gcttttattc ttgatgagtt atggaaaagg cataaaaaaa agagagaaga 2040 agattaatct tcttctctct tttttttatg cctttgtcct ctatggggtt ccgtcaggaa 2100 aatgcggatt tacaacgtta aactattttt gggaaacaaa aacctgcttc tccaactcta 2160 agcagaagaa gcaaggtgag gtaatatttt cacttgccag aatcacgagg tggattcacc 2220 ataagaaaca ctctaaaaat tggtacagat acgaatccat ttgtacagaa cgtaaagtaa 2280 aggatttatg aataaacgta tacaaaattg gaataatatc tttgaaaata accaaggagg 2340 attgaatatg tcattagtac cttatgatcc atttagacaa ttatcaaata tgagaagaga 2400 attcgatcgt ttcttttcgg aattaccaat ttcgtttgac aatgaacatg gtataggtgg 2460 gattcgagtt gatgttcatg aaactgagaa tgaggttgtg gcaacatgtg atttacctgg 2520 tcttgaaaag aaagaagatg tagatattga tatacaaaat aacagattaa gcattagtgg 2580 ttctatcaag cgtaccaatg aaataaaaga agaaaatatg ttaaaaaagg aacgctatac 2640 aggtcgtttt caacgtatga taacacttcc aagccccgtt tcacatgatg gggttaaagc 2700 tacgtacaaa aatggaatac ttgaaataac aatgccaaaa gtggcgaagg acgtaaaaaa 2760 gaagatagat gtaagtttcc agtaacatga gtaaattgct actggtcatg aaagatttcg 2820 ggtatgacta catgcaggaa ttagctaaag aaagtcctga tttggtggat ttgataattt 2880 taactattca ttcacctgaa caacaaaaga aaaaggtcat ttctgagtta acagataata 2940 ttaagaaaag ctttttataa atctgataag gatcataggc tttatataag ctaataaata 3000 atcattatag tacctaaatt gtaattaatt catcgaaaga gaaagacagg ttaaataact 3060 tgtctttttt gttttgcaat taacaaaaga ttatttggtc aatctttgta ataacatgtt 3120 tactataacc aacataaata attatgaata acaataccat aattttgtaa tgctaactgt 3180 aaactaacat tgtgagagga tattatgtac ataaaggggt accgtcagga aaataggctt 3240 agcgttgtaa atccgcattt tcctgacggt gtacctctat ggggaagatc cttataaaat 3300 gagaaagtag atgcacggtt tcacagcata ctttctgtaa ttgaaaattt gacacacaga 3360 acaatttgtt catgcggtca aatgctgcgg gagtagcaag ggtttttccg attttctgcc 3420 gatctctcat cggcggaaaa gtcgggtcgg cagacagccg acaagtggca cgaactttcg 3480 atgcgatagc gagaatgaga gagccaccag caccgcaggt cgcacgtcca aatttgccat 3540 gggcataatt tggtgtagtg cgttacacca aagataaact ttgtgttacc ataaccccta 3600 tacagtggtc tgaatcgggg gtttttctca tggcaaatta tgcagtcatc aggatggaaa 3660 aatacaaaaa agatagattg aatggaacgc aaaaacacaa tcagcgggag tttcaaaaaa 3720 gcaaaaatga aaatattgat cgggagcgga cgcacttaaa ttatgatcta gtgaacgaga 3780 aaccgattag ctattcaaaa gcgattcatg aaaaaattga ggggcgagtc aaacggaagg 3840 tccgagcgga tgctgttttg gtcagcgaat ttttgatcac ggcaagtcct gactatatga 3900 atgggctgag cgatgaggag cagcggcgct attttgaaac agcggttgat catttgaaag 3960 agaaatacag cgctgaaaac atgctttatg ctacagtcca tatggatgaa gcgactcctc 4020 atatgcatgt tggtattgta ccgatcacag aggacggccg actctctgcg aaagattttt 4080 ttaatggcaa attgaagatg aaagccattc aagatgattt tcatcggcac atggttgaaa 4140 acggttttga cctggtgcgc ggcgaaccaa gcgaaaagaa gcatgagaat gttcaccagt 4200 ataaaataaa tcagcggcaa gcggagcttg agcggcttaa tgctgaaatt gctttaaagg 4260 aaaagcagag agaggaactg gaaaagcaaa acaaagctgt tcaagcagtt atagaagtga 4320 aaaaagaatc gctgacagct aaggctgaag agttgaaaat gccgactatt gaacatgaaa 4380 aagcgtggct caaaaaggat aaagtcattg tgccagagcg ggaactccat gctttgtatg 4440 cctatgcgga gcagaaaact aaaacggcag ccgagttggc ggggcaattg aagtcggaaa 4500 cgcaggaaaa ggagcgctgg cagtctatcg cccggcagga agcagatcgg gcggatgaaa 4560 aagaccaacg gcttcaggaa ctgcagagta ggatccattc agaagttgaa gcgtccaaaa 4620 aggaaatgcg gcgcaagctt gcaaaggaat ttacggaaga acagcgtcag gatcttcggc 4680 aggaagtgaa agaggaactg acgactttac gaacggaaaa cgaggaactg tcagctgaaa 4740 ataaagtttt gatcattcaa agaaatagcg aagctgcgga gagcctaaaa ctaaaacagg 4800 aacttgataa gagaaacggg cagtatgctg aggttttgag tttcgccaag aagcagaatc 4860 aaacgcttga aaaagtggct ggagaaaaca aggcgttaaa aaaagaaaat aagacactaa 4920 aagagagagt tgccgtactg gaacaatgga aagacaaaat ggttcagtgg gctaaagaaa 4980 aattaccaaa gatgcggaaa ttagcggcat cgtttttccg tacggctgga atgcctagag 5040 aagccaataa atacaaggac aatgaattag agcggtgaaa acgggcaatc aattgccctt 5100 ccaaaatttc caccactttt tttgtttggt ggctgcgatc attttttgtg tctcaaggga 5160 ctcacgtaat gcagcggtga gtgtctcgtg cctttcttgc tgtctttttt cgaatcgttc 5220 catccgttct gccatgtggc ggttgaattc ttcctgtcgt ttcatgaatt cgactaaagg 5280 attgtcctgt agcgatgtag cggtatccga tatggtcagt ttagaacgat ataagcttgc 5340 tatgtgcttt accgtttcgt cgagtgagtg tccattgatc ttagtcattg tacatagata 5400 ctctaaagtc tttacgtcat cctcggtata gagtcgccat cctttcgaat ctttattgaa 5460 cgagtagcct tgttcttcaa gcatactggc atacttgcgg acagttactg gctctatacc 5520 gaggtgtttt gcgacgtcct ttgatgataa tttgattccc atatccataa cgtatcacct 5580 cgtaaaaagg gttcgctata gtgagggcaa agccctttat ttagagccat ttaaaaagaa 5640 agctgttccg ttcttttatc aggttgggac tgtatttagt ttatggggga gtgccaatga 5700 gtgattttga aacaggaatg agatatgtaa gagcaaccct tggctttgag ggtttagtgc 5760 tgacggaaga agaggaaaag cttttagaaa gacggtttca tggagaaatc acagaggaag 5820 aatacataca aaaagcgttt gagctttctt tgatgtgatg tacctgcagg ggcttataat 5880 attcatagct gttgttagaa cctaaaccct tttaccgttt ttggcggtga gggttctttt 5940 tttttaggca gtgatgcgat tttggcgtga gtcaacggta accggaccgt agggaggatt 6000 aaggagttga ctcgctcagc gccacccgaa ccctttcagc actcaaacaa acccgtttgt 6060 ttgacgccaa ccggcgaggg agccccccga agatgcgggg ggttgggggg attgaatgct 6120 ggcatccaac ggccgtccgt tggtgggttt gggcaaagcc caagaactgt tgcaaggctc 6180 gttgagaata aagaatgctt ttcaggatgc ttagaatcgt ttctgagagc ttcaaataaa 6240 aaagatgacc ttttataggg ggaagctctt aaaattgaat gtaggggcat ttaaacacgt 6300 ttaaaaataa aaaaagcaga ctctttagag tccgccttgt tatttttaac ccagtgctcc 6360 attttcggct gtttggaaat cttttgagat gccgaaccat ccattttctt ttgttccatg 6420 aaaaaagtgc ttttggatgc ttaaaaaggc tttttcgtat aaaaaaagcc gatttttgaa 6480 aaaaaaatct cccccccctg cgggggaaga atggttttga tctttgggtt ttaggtttta 6540 aaaaaagccg gctgttttca gccggctttt tttcgatttt ggcggagccg aaatcgggtc 6600 ttttcttatc 6610 //