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Title:Structure of the bacterial flagellar motor from Borrelia
Authors:M.Kudryasheva,M.Cyrklaff,R.Wallich,W.Baumeister,F.Frischknecht
Sample:Cryo preserved Borrelia spirochetes
Aggregation state:Individual structure (46 angstroms resolution)
Red flagLatest update:2011-09-09
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: Cryo preserved Borrelia spirochetes
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1cellular-componentBacterial flagellar motorfalseunidentified
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM300FEG/T300 kVSPOT SCANBRIGHT FIELD mm10000 nm10000 nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN-60°60° eV K K K mrad e/Å2
Processing
Software:EM-package, TOM for matlab
CTF correction:no
Resolution by author:46 Å
Resolution method:1 bit
Processing details:The final map is calculated from 107 single motors, 16 fold rotational symmetry applied
Unit cell:
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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