The EMBL Nucleotide Sequence Database was frozen to make Release 61
on 03-DEC-1999. The release contains 5,303,436 sequence entries comprising
4,508,169,737 nucleotides. This represents an increase of about 27% over
Release 60. A breakdown of Release 61 by division is shown below:
Division |
Entries |
Nucleotides |
| Bacteriophage |
1,493
|
3,772,075
|
| EST |
3,352,471
|
1,280,533,161 |
| Fungi | 35,387 | 65,382,544 |
| GSSs | 1,135,096 | 584,522,641 |
| HTG | 11,236 | 1,137,902,159 |
| Human | 101,754 | 611,811,099 |
| Invertebrates | 43,125 | 178,290,696 |
| Organelles | 53,615 | 45,527,348 |
| Other Mammals | 22,071 | 20,697,174 |
| Other Vertebrates | 20,197 | 23,184,728 |
| Patents | 188,650 | 59,188,603 |
| Plants | 44,530 | 143,428,159 |
| Prokaryotes | 68,545 | 164,563,553 |
| Rodents | 49,195 | 74,393,858 |
| STSs | 90,061 | 32,796,622 |
| Synthetic | 3,355 | 7,987,485 |
| Unclassified | 1,454 | 2,274,354 |
| Viruses | 81,201 | 71,913,478 |
| total | 5,303,436 | 4,508,169,737 |
In *addition* to the cumulative.dat.gz files we make available on our FTP server, we now also split that dataset into smaller parts, namely:
cum_1.dat.gz all the cumulative files except the data
in files below
cum_est1.dat.gz ESTs
cum_htg1.dat.gz HTG
cum_hum1.dat.gz human (non-gss,non-HTG, non-EST)
cum_gss1.dat.gz GSS
We chose this data division:
- because it represents the current major division of data in the updates
- to try to keep the files below 2GB for as long as possible, eg before
needing cum_hum2 etc - to avoid frequent
changes. Due to the Draft Human Genome effort we expect the
HTG division to be a likely candidate for further
splitting in the near future.
These filenames should last a while and contain some data even soon after a new full release. SRS icarus files (for example) should not need constant changing, and when they do we should be able to see it coming well beforehand.
Those who 'mirror' our ftp.ebi.ac.uk/pub/databases/embl/new directory
may wish to have either the full cumulative.dat.gz or the cum_* set ignored,
in order to save some netwidth. The data contained is the same - we build
the cum_* files from the cumulative.dat.gz file afresh each day. There
is no correspondence between, cum_est1.dat and the est1.dat file of the
full release.
The EBI SRS server now has the EMBLNEW dataset using these cum_* files.
EMBL links to other databases: DATABASE Number of links RZPD 684241 TrEMBL 256736 AGIS 160085 Swiss-Prot 134427 MaizeDB 43859 IMGT/LIGM 28305 MENDEL 21033 FLYBASE 10763 GDB 8430 MGD 8079 TRANSFAC 6620 SGD 6029 IMGT/HLA 2168 EPD 1198 -------- ------- Total 1371973 Example: DR RZPD; IMAGp998G19998; 16-Jul-1999.1.3 Database Files
In order to keep the size of the data files within reasonable limits for handling purposes, additional division files will be added in subsequent releases as appropriate.
The EST division has been split into into 34 files. (est1.dat-est34.dat).
The GSS division has been split into 12 files (gss1.dat-gss12.dat).
The HUM division has been split into 5 files. (hum1.dat-hum5.dat).
The HTG division has been split into 3 files. (htg1.dat-htg3.dat).
A consortium of 5 major sequencing centres (Sanger, Baylor,WashU, Whitehead,
DOE) are involved in establishing the draft human genome. The Sanger Centre
(UK) is the European participant producing about 33% (~1 Gb) of the high-through-put
draft genome sequence data (HTG phase 1) including high-throughput automatic
annotation. The project is expected to be completed by February 2000. As
a direct result the Release 61 High-Throughput-Data division (HTG) now
includes over 1,137 Mbases (11,236 entries), compared to 544 Mbases (4,781
entries) in Release 60 (Sep 1999).
2.1 Replacement of organelle-related qualifiers with new qualifier
/organelle
A large variety of organelle qualifiers currently exist: /mitochondrion, /chromoplast, /chloroplast, etc. Starting with EMBL Release 62 (March 2000), these qualifiers will all be incorporated into a single new qualifier, with a controlled value format. The preliminary description of this qualifier is as follows:
Qualifier /organelle="" Definition type of membrane-bound intracellular structure from which the sequence was obtained Value format mitochondrion, nucleomorph, plastid, mitochondrion:kinetoplast, plastid:chloroplast, plastid:apicoplast, plastid:chromoplast, plastid:cyanelle, plastid:leucoplast, plastid:proplastid, Examples /organelle="mitochondrion" /organelle="nucleomorph" /organelle="plastid" /organelle="mitochondrion:kinetoplast" /organelle="plastid:chloroplast" /organelle="plastid:apicoplast" /organelle="plastid:chromoplast" /organelle="plastid:cyanelle" /organelle="plastid:leucoplast" /organelle="plastid:proplastid" Comments modifier text limited to values from controlled list
2.2 Mutation and Allele features
to be discontinued
At the May 1999 collaborative DDBJ/EMBL/GenBank meeting the usage of
change feature keys 'variation', 'mutation', and 'allele' was reconsidered.
It was decided to redefine anduse 'variation' to describe alleles,
RFLP's, and other naturally occuring mutations and polymorphisms. Variability
arising as a result of genetic manipulation (e.g. site directed mutagenesis)
will be described with the misc_difference feature.
Based on this collaborative decision, the EMBL database will at Release 62 (March 2000) convert feature keys mutation and allele to variation, while feature keys 'mutation' and 'allele' will be discontinued.
For further details on feature keys and qualifiers please see the new
version of the Feature Table Definition Document (v2.2) available
from the EBI servers after Dec 21st, 1999.
(http://www.ebi.ac.uk/emblDocumentation/FT_definitions/feature_table.html)
2.3 Value format of /number to be
modified
At the May 1999 collaborative DDBJ/EMBL/GenBank meeting it was decided to modify /number to allow better representation of alternative exon designations (e.g. 6B). Qualifier /number= Definition a number to indicate the order of genetic elements (e.g., exons or introns) in the 5' to 3' direction Value format unquoted text Example /number=4 /number=6B Comment text limited to integers, letters or combination of integers and/or letters represented as an unquoted single token (e.g. 5a, XIIb). Any additional terms should be included in /standard_name. Example: /number=2A /standard_name="long"
This will be implemented with Release 62 (March 2000)
2.4 /evidence made legal on all
feature keys
Starting from Release 62 (March 2000) /evidence will be legal on all feature
An experimental directory representing genome data has been available from the EBI anonymous FTP server in directory
including a number of files for each genome: a file listing construct information and all accession numbers relevant to the project, the complete single entry in EMBL format (DNA and features), the complete DNA sequence in FASTA format, e.g.
in ftp://ftp.ebi.ac.uk/pub/databases/embl/genomes/Eubacteria/bsubtilis/
README
bsubt.con
bsubt.embl
bsubt.embl.Z
bsubt.fasta
bsubt.fasta.Z
Efforts are now undertaken to develop a new database division (CON)
to represent these complete genomes, or other long sequences constructed
from segment entries. The CON division will contain construct information,
accession numbers and sequence locations involved in building the constructs.
Additionally, the according complete entries including descriptive information , references, features, DNA sequence will be linked, searchable and retrievable through SRS and available for BLAST and FASTA homology searching.3 SEQUENCE SUBMISSION SYSTEMS
3.1 Checking Sequence Data For Vector Contamination
We urge submitters to remove vector contamination from sequence data before submitting to the database. To assist submitters the EBI is providing a Vector Screening Service using the latest implementation of the BLAST algorithm and a special sequence databank known as EMVEC. EMVEC is an extraction of sequences from the SYNthetic division of EMBL containing more than 2000 sequences commonly used in cloning and sequencing experiments. EMVEC is by no means a complete vector databank but EBI believes it is representative of the kind of material used in modern sequencing and should be useful to submitters. The databank will be updated with each release of EMBL and made publicly available on the EBI's ftp (ftp.ebi.ac.uk) server for those who wish to have it.
The interactive WWW service can be found at:
http://www.ebi.ac.uk/emblSubmission/webin.html
http://www.ebi.ac.uk/blastall/vectors.html
The results will list sequences producing significant alignments and associated information like vector name, score, alignment etc
3.2 WebIn - WWW Sequence Submission System
WebIn is the preferred WWW Sequence Submission System for submitting nucleotide sequence data and associated biological information to the EMBL Nucleotide Sequence Database at the European Bioinformatics Institute (EBI). To access WebIn at the EBI please use the following URL:
http://www.ebi.ac.uk/emblSubmission/webin.html
Database entries created by the new WWW submission tool and submitted to the EMBL Nucleotide Sequence Database at the EBI will be exchanged and shared among the International Collaboration of Nucleotide Sequence Databases (DDBJ/EMBL/GenBank).
WebIn guides the user through a sequence of WWW forms allowing the submission of sequence data and descriptive information in an interactive and easy way. All the information required to create a database entry will be collected during this process:
1 Submitter Information
2 Release Date Information
3 Sequence Data, Description and Source Information
4 Reference Citation Information
5 Feature Information (e.g. coding regions, regulatory signals etc.)
EBI staff will process data submissions within 2 working days and send the database accession number(s) assigned to your data to your e-mail address.
With the aim to make bulk sequence submission highly efficient and less time consuming for the submitters, a prototype of a new web-based bulk sequence submission system can now be accessed from WEBIN. Authors planning to submit a large number of similar sequences (i.e.,>25) are presented with an option for "Bulk WebiN Submission". When choosing the bulk path, submitters carry on the usual WEBIN submission procedure until having finished a first and single representative sequence. During the submission process database staff will interactively assist in making the submission of this specific data as convenient as possible, thus saving the author the time and effort required to complete numerous submission events individually.
Alternatively, authors planning to submit very large numbers
of similar sequences should contact the database before submitting the
data. Database staff will then assist in making the submission of this
data as convenient as possible, thus saving the author the time and effort
required to complete numerous submission events individually. When contacting
database staff, authors should indicate the number of sequences they plan
to submit. Database staff will create series of templates and communicate
these to the author for completion with just the information unique to
each sequence required. These templates, once resubmitted, will then be
processed en masse by
database curators.
Please contact database staff if you require further information.
e-mail: datasubs@ebi.ac.uk
Tel: +44-1223-494499
Fax: +44-1223-494472
3.4 SEQUIN - Stand-alone Submission Program
Sequin is the multi-platform (Mac/PC/Unix) stand-alone software tool developed by the NCBI for submitting entries to the EMBL, GenBank, or DDBJ sequence databases. The Sequin program, along with detailed downloading and installation instructions plus general information are available from the EBI via WWW browser and anonymous FTP.
http://www.ebi.ac.uk/Sequin/
ftp://ftp.ebi.ac.uk/pub/software/sequin/
3.5 Sequence Alignment Submissions
The EBI accepts submissions of alignment data (e.g. from phylogenetic and population analysis etc..) of both nucleotide or amino-acid sequences. This data is assigned an alignment number (e.g. ds38200) by database staff which is then communicated to the submitter. We suggest that this number is quoted in the resulting publication.
Alignment data and associated information are made available via EBI's network servers (see below).
ALIGNMENT FORMATS:
As well as your alignment data we require information describing your alignment (see table below) Please provide information for all fields.
| Description Field | Information required |
| TITLE: | Title of alignment |
| SUBMITTER: | Name, Affiliation, Phone, Fax, Email |
| RELEASE DATE: | Public Immediately / if Confidential please provide hold date |
| CITATION: | If known please provide complete Author list, Title, Journal, Year of publication, Page numbers |
| ALIGNMENT METHOD: | Method of alignment and format submitted, parameters of alignment sequences used (if appropriate) |
| DESCRIPTION OF SYMBOLS: | e.g. Gaps indicated by a dash '-' |
| DESCRIPTION OF ALIGNMENT: | Describe sequences aligned, including accession numbers (if known) and abbreviation of clones or taxon used in alignment file. If your alignment contains sequences derived from multiple taxoonomic sources, please provide the full name of each organism |
| FILE FORMAT: | We are currently updating and improving both the access to and alignment
output of this archive due to an increase in the submission of alignment
data. The compilation of text files and the issue of format standardisation
are undergoing review and are being discussed by the database staff, external
users and experts in the field.
We suggest submission in STANDARD ALIGNMENT FORMATS eg. (NEXUS, PHYLIP, ClustalW etc) or Sequin output. |
A sample alignment in NEXUS format can be viewed at ftp://ftp.ebi.ac.uk/pub/databases/embl/align/ds32096.dat
NOTE 1: Alignments can be created within Sequin or imported into Sequin from files in a standard alignment format like NEXUS or PHYLIP.
NOTE 2: If reporting new primary sequence data, we suggest that you submit the complete individual sequence files (e.g. via Sequin or Webin), in order to include the sequence data as individual entries in the database. If gaps have been introduced for the alignment, please leave them out when sending the individual sequence files.
SENDING ALIGNMENT DATA to the EMBL Nucleotide Sequence Database
Sequence Alignment Data can be sent to the Nucleotide Sequence Database
by Electronic mail to DATASUBS@EBI.AC.UK
ACCESSING ALIGNMENT DATA
Alignment data and additional information are available via the EBI
servers:
EBI WWW server: http://www.ebi.ac.uk/emblSubmission/alignment.html
ftp://ftp.ebi.ac.uk/pub/databases/embl/align/ EBI FTP server: by anonymous FTP from FTP.EBI.AC.UK in directory /pub/databases/embl/align EBI File server: by sending an e-mail message to netserv@ebi.ac.uk including the line HELP ALIGN or GET ALIGN:DS8200.DAT3.6 Further Submission Information
To help and guide submitters in annotating their sequences, two new online guides are now available via hyperlinks from within WEBIN: EMBL Annotation Examples and EMBL Features and Qualifiers. The annotation examples consist of a list of EMBL approved feature table annotations for common biological sequences. The EMBL Features and Qualifiers is a complete list of feature table key and qualifier definitions providing detailed descriptions, mandatory and optional qualifiers and usage examples.
For further information on submission of sequence data to the EMBL Nucleotide Sequence Database please access:
http://www.ebi.ac.uk/emblSubmission/
or contact database staff at:
EMBL Nucleotide Sequence Submissions
e-mail: datasubs@EBI.AC.UK
telephone: +44-1223-494499
telefax: +44-1223-494472
4 CITING THE EMBL NUCLEOTIDE SEQUENCE DATABASE
We encourage authors to include a reference to the EMBL Database in publications related to their research.
When citing data in the EMBL Database, we suggest to give the according primary accession number(s) and the publication in which the sequence first appeared. For unpublished data, we suggest to contact the original submitters for recent publication information or revisions of the data.
We suggest to also provide a reference for the EMBL Database itself. Our recent publication in Nucl. Acids Res., 1999, Vol. 27 (1), 18-24., which describes the EMBL database, should be cited:
Stoesser, G., Tuli, M.A., Lopez, R., and Sterk, P.. The EMBL Nucleotide Sequence Database Nucl. Acids Res., 27:18-24(1999)
Example: The numbers in parentheses refer to the REFERENCE in the EMBL database entry, and to the EMBL citation above.
"Sequence entry X56734 (1) has been retrieved from the EMBL Database (2) and showed significant sequence similarity to ..."
(1) Oxtoby E., et al., Plant Mol. Biol. 17:209-219(1991).
(2) Stoesser, G., et al., Nucl. Acids Res. 27:18-24(1999)
Computer users with access to Internet (directly or via a gateway) can obtain copies of database entries, documentation or the data submission form, by sending commands to a file server running on the computer systems at EBI. New and updated EMBL nucleotide sequence entries are made available on the server on a daily basis.
To use this facility, send file server commands (as electronic mail) to the address Netserv@EBI.AC.UK. Each line of the mail message should consist of a single file server request.
The most important file server request, to get started, is:
HELP
If the file server receives this command, it will return a help file to the sender, explaining in some detail how to use the facility. For example, to request a copy of the nucleotide sequence with accession number X12399, use the commands:
GET NUC:X12399
The file server offers various other services, (eg., access to nucleotide and protein sequence data, protein structure data, software), details of which are provided in the HELP file.
An alternative method of accessing the EBI archives is to use the Internet file transfer protocol (ftp). Researchers with direct access to the Internet can use the FTP program on their local machine to connect to the host FTP.EBI.AC.UK and enter the user name "anonymous" and their email address as password. The directory pub/help contains detailed information about the data available from the EBI anonymous FTP server which includes the complete EMBL Nucleotide Sequence Database releases as well as daily and weekly updates and a cumulative update file (in UNIX-compressed format) in the following directories:
EMBL quarterly release: pub/databases/embl/release
EMBL updates: pub/databases/embl/new
5.3 World Wide Web (WWW) Server
The EBI operates a WWW server with URL http://www.ebi.ac.uk/ which gives access to information about the EBI and it's products and services. Nucleotide sequences can be retrieved by a simple query by accession number, or more complex queries can be contructed using an SRS WWW databank browser. Nucleotide sequences can also be submitted to the database using the interactive submission system WebIn at URL:
http://www.ebi.ac.uk/emblSubmission/webin.html
The EBI offers two network servers for sequence similarity searches via electronic mail or interactive WWW forms:
| FASTA | based on W. Pearson's FASTA algorithm. Allows local similarity searches of protein and nucleotide sequence databases. Send "help" to fasta@ebi.ac.uk or use URL http://www.ebi.ac.uk/fasta33/ |
| BLAST | based on the NCBI and WU-BLAST software Send "help" to blast@ebi.ac.uk or use URL http://www.ebi.ac.uk/blast2/ |
| BLITZ | BLITZ allows very fast searches of protein sequence databases for local similarities using an exhaustive Smith-Waterman matching algorithm. Compugen's BIC_SW software is running on a Biocellerator (BIC-2) Send "help" to Blitz@EBI.AC.UK or use URL http://www.ebi.ac.uk/bic_sw/ |
The release contains the files shown below, in the order listed. File sizes are given as numbers of records.
File Number |
File Name |
Description |
Number of Records |
| 1 | DELETEAC.TXT | Deleted accession numbers | 32574 |
| 2 | ACNUMBER.NDX | accession numbers | 5350598 |
| 3 | CITATION.NDX | Citation Index | 728141 |
| 4 | DIVISION.NDX | Division Index | 23 |
| 5 | KEYWORD.NDX | Keyword index | 1994758 |
| 6 | SHORTDIR.NDX | Short Directory Index | 12854551 |
| 7 | SPECIES.NDX | Species index | 1857007 |
| 8 | EST1.DAT | EST Sequences | 6917140 |
| 9 | EST2.DAT | EST Sequences | 6868828 |
| 10 | EST3.DAT | EST Sequences | 6918600 |
| 11 | EST4.DAT | EST Sequences | 6762605 |
| 12 | EST5.DAT | EST Sequences | 6841235 |
| 13 | EST6.DAT | EST Sequences | 6877091 |
| 14 | EST7.DAT | EST Sequences | 6614170 |
| 15 | EST8.DAT | EST Sequences | 6655889 |
| 16 | EST9.DAT | EST Sequences | 6452009 |
| 17 | EST10.DAT | EST Sequences | 6816372 |
| 18 | EST11.DAT | EST Sequences | 6886486 |
| 19 | EST12.DAT | EST Sequences | 6922015 |
| 20 | EST13.DAT | EST Sequences | 6956466 |
| 21 | EST14.DAT | EST Sequences | 7043033 |
| 22 | EST15.DAT | EST Sequences | 6672696 |
| 23 | EST16.DAT | EST Sequences | 6264962 |
| 24 | EST17.DAT | EST Sequences | 6910980 |
| 25 | EST18.DAT | EST Sequences | 7692962 |
| 26 | EST19.DAT | EST Sequences | 9798091 |
| 27 | EST20.DAT | EST Sequences | 7920006 |
| 28 | EST21.DAT | EST Sequences | 7206489 |
| 29 | EST22.DAT | EST Sequences | 6048967 |
| 30 | EST23.DAT | EST Sequences | 5590064 |
| 31 | EST24.DAT | EST Sequences | 6582879 |
| 32 | EST25.DAT | EST Sequences | 6216954 |
| 33 | EST26.DAT | EST Sequences | 5690325 |
| 34 | EST27.DAT | EST Sequences | 5720526 |
| 35 | EST28.DAT | EST Sequences | 6412340 |
| 36 | EST29.DAT | EST Sequences | 6732959 |
| 37 | EST30.DAT | EST Sequences | 6336548 |
| 38 | EST31.DAT | EST Sequences | 7081695 |
| 39 | EST32.DAT | EST Sequences | 7038538 |
| 40 | EST33.DAT | EST Sequences | 6773524 |
| 41 | EST34.DAT | EST Sequences | 2980098 |
| 42 | FUN.DAT | Fungi Sequences | 3244162 |
| 43 | GSS1.DAT | Genome Survey Sequences | 6106747 |
| 44 | GSS2.DAT | Genome Survey Sequences | 6066673 |
| 45 | GSS3.DAT | Genome Survey Sequences | 6225473 |
| 46 | GSS4.DAT | Genome Survey Sequences | 6610123 |
| 47 | GSS5.DAT | Genome Survey Sequences | 6414584 |
| 48 | GSS6.DAT | Genome Survey Sequences | 6538457 |
| 49 | GSS7.DAT | Genome Survey Sequences | 6506858 |
| 50 | GSS8.DAT | Genome Survey Sequences | 6485960 |
| 51 | GSS9.DAT | Genome Survey Sequences | 6919213 |
| 52 | GSS10.DAT | Genome Survey Sequences | 6201759 |
| 53 | GSS11.DAT | Genome Survey Sequences | 6123524 |
| 54 | GSS12.DAT | Genome Survey Sequences | 1903117 |
| 55 | HTG1.DAT | High Throughput Genome Sequences | 8254798 |
| 56 | HTG2.DAT | High Throughput Genome Sequences | 5965845 |
| 57 | HTG3.DAT | High Throughput Genome Sequences | 6243891 |
| 58 | HUM1.DAT | Human Sequences | 8409778 |
| 59 | HUM2.DAT | Human Sequences | 4158106 |
| 60 | HUM3.DAT | Human Sequences | 2627520 |
| 61 | HUM4.DAT | Human Sequences | 2072299 |
| 62 | HUM5.DAT | Human Sequences | 152714 |
| 63 | INV.DAT | Invertebrate Sequences | 5883751 |
| 64 | MAM.DAT | Other Mammal Sequences | 1556006 |
| 65 | ORG.DAT | Organelle Sequences | 3805823 |
| 66 | PATENT.DAT | Patent Sequences | 7343825 |
| 67 | PHG.DAT | Bacteriophage Sequences | 192470 |
| 68 | PLN.DAT | Plant Sequences | 5342838 |
| 69 | PRO1.DAT | Prokaryote Sequences | 6022265 |
| 70 | PRO2.DAT | Prokaryote Sequences | 1911256 |
| 71 | ROD.DAT | Rodent Sequences | 4102930 |
| 72 | STS.DAT | STS Sequences | 5755715 |
| 73 | SYN.DAT | Synthetic Sequences | 330695 |
| 74 | UNC.DAT | Unclassified Sequences | 122805 |
| 75 | VRL.DAT | Viral Sequences | 5986921 |
| 76 | VRT.DAT | Other Vertebrate Sequences | 1499665 |
SRS indices can be found on the FTP server in the srs directory ftp://ftp.ebi.ac.uk/pub/databases/embl/release/srs/. Please read the README file for details
DATABASE GROWTH TABLE
The following table shows the growth of the EMBL Nucleotide Sequence Database at each release.
Release |
Month |
Entries |
Nucleotides |
| 1 | 06/1982 | 568 | 585433 |
| 2 | 04/1983 | 811 | 1114447 |
| 3 | 12/1983 | 1481 | 1654863 |
| 4 | 08/1984 | 1698 | 2147205 |
| 5 | 04/1985 | 2378 | 2874493 |
| 6 | 08/1985 | 4835 | 4567592 |
| 7 | 12/1985 | 5789 | 5622638 |
| 8 | 04/1986 | 6395 | 6353040 |
| 9 | 09/1986 | 7630 | 7813214 |
| 10 | 12/1986 | 8817 | 9766948 |
| 11 | 04/1987 | 11621 | 12189783 |
| 12 | 07/1987 | 12706 | 13638061 |
| 13 | 10/1987 | 14397 | 16023478 |
| 14 | 01/1988 | 15344 | 17272160 |
| 15 | 05/1988 | 17961 | 20318442 |
| 16 | 08/1988 | 19592 | 22625941 |
| 17 | 11/1988 | 20695 | 24211054 |
| 18 | 02/1989 | 22938 | 27249830 |
| 19 | 05/1989 | 24365 | 29066676 |
| 20 | 08/1989 | 26223 | 31240948 |
| 21 | 11/1989 | 28679 | 34748087 |
| 22 | 02/1990 | 31508 | 38165786 |
| 23 | 05/1990 | 34902 | 42923803 |
| 24 | 08/1990 | 37784 | 47354438 |
| 25 | 11/1990 | 41580 | 52900354 |
| 26 | 02/1991 | 43745 | 55859549 |
| 27 | 05/1991 | 46871 | 59915244 |
| 28 | 09/1991 | 54558 | 70448052 |
| 29 | 12/1991 | 5765 | 75400487 |
| 30 | 03/1992 | 63378 | 83574342 |
| 31 | 06/1992 | 72481 | 94390065 |
| 32 | 09/1992 | 79377 | 101292310 |
| 33 | 12/1992 | 89100 | 111413979 |
| 34 | 03/1993 | 99591 | 121420828 |
| 35 | 06/1993 | 108973 | 131880111 |
| 36 | 09/1993 | 127933 | 145401156 |
| 37 | 12/1993 | 146576 | 158171400 |
| 38 | 03/1994 | 167777 | 177550115 |
| 39 | 06/1994 | 182615 | 192195819 |
| 40 | 09/1994 | 209352 | 211017104 |
| 41 | 12/1994 | 230950 | 226259607 |
| 42 | 03/1995 | 303206 | 262559786 |
| 43 | 06/1995 | 420111 | 315840053 |
| 44 | 09/1995 | 506190 | 363273777 |
| 45 | 12/1995 | 622566 | 427620278 |
| 46 | 03/1996 | 701246 | 473691480 |
| 47 | 06/1996 | 827174 | 550739395 |
| 48 | 09/1996 | 928067 | 608931850 |
| 49 | 12/1996 | 1047263 | 696183789 |
| 50 | 03/1997 | 1187455 | 789755858 |
| 51 | 06/1997 | 1432941 | 931351601 |
| 52 | 10/1997 | 1787004 | 1181167498 |
| 53 | 12/1997 | 1917868 | 1281391651 |
| 54 | 03/1998 | 2125225 | 1427634373 |
| 55 | 06/1998 | 2330040 | 1607673907 |
| 56 | 09/1998 | 2689618 | 1904091473 |
| 57 | 12/1998 | 3046471 | 2164718256 |
| 58 | 03/1999 | 3272064 | 2355200790 |
| 59 | 06/1999 | 3952878 | 2924568545 |
| 60 | 09/1999 | 4719266 | 3543553093 |
| 61 | 12/1999 | 5303436 | 4508169737 |