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2010

Defining topological features of membrane proteins by nanoelectrospray ionisation mass spectrometry.

Jones LN, Baldwin SA, Henderson PJ, Ashcroft AE
Rapid Commun Mass Spectrom (24) Mar 2010 276-284

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2009

Structural analysis of uniformly (13)C-labelled solids from selective angle measurements at rotational resonance.

Patching SG, Edwards R, Middleton DA
J Magn Reson (199) Oct 2009 242-6
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The lateral gate of SecYEG opens during protein translocation.

du Plessis DJ, Berrelkamp G, Nouwen N, Driessen AJ
J Biol Chem (284) Aug 2009 15805-14
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The role of biomacromolecular crowding, ionic strength, and physicochemical gradients in the complexities of life's emergence.

Spitzer J, Poolman B
Microbiol Mol Biol Rev (73) Aug 2009 371-88

Crystal structure of a yeast aquaporin at 1.15 angstrom reveals a novel gating mechanism.

Fischer G, Kosinska-Eriksson U, Aponte-Santamaria C, Palmgren M, Geijer C, Hedfalk K, Hohmann S, de Groot BL, Neutze R, Lindkvist-Petersson K
PLoS Biol (7) Aug 2009 e1000130

Selenomethionine incorporation in proteins expressed in Lactococcus lactis.

Berntsson RP, Alia Oktaviani N, Fusetti F, Thunnissen AM, Poolman B, Slotboom DJ
Protein Sci (18) July 2009 1121-7
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A method for site-specific labeling of multiple protein thiols.

Kuiper JM, Pluta R, Huibers WH, Fusetti F, Geertsma ER, Poolman B
Protein Sci (18) July 2009 1033-41
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The structural basis for peptide selection by the transport receptor OppA.

Berntsson RP, Doeven MK, Fusetti F, Duurkens RH, Sengupta D, Marrink SJ, Thunnissen AM, Poolman B, Slotboom DJ
EMBO J (28) July 2009 1332-40
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Attenuated total reflection-FT-IR spectroscopic imaging of protein crystallization.

Chan KL, Govada L, Bill RM, Chayen NE, Kazarian SG
Anal Chem (81) July 2009 3769-75
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Engineering of Ion Sensing by the Cystathionine {beta}-Synthase Module of the ABC Transporter OpuA.

Mahmood NA, Biemans-Oldehinkel E, Poolman B
J Biol Chem (284) July 2009 14368-76

The quest for a better resolution of protein-translocation processes. Conference on the Control, Co-ordination and Regulation of Protein Targeting and Translocation.

Borgese N, Driessen AJ, Rapaport D, Robinson C
EMBO Rep (10) June 2009 337-42
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Structural and functional analysis of SoPIP2;1 mutants adds insight into plant aquaporin gating.

Nyblom M, Frick A, Wang Y, Ekvall M, Hallgren K, Hedfalk K, Neutze R, Tajkhorshid E, Tornroth-Horsefield S
J Mol Biol (387) June 2009 653-68
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Increased hydrophobicity at the N-terminus/membrane interface impairs gating of the SCID-related ORAI1 mutant.

Derler I, Fahrner M, Carugo O, Muik M, Bergsmann J, Schindl R, Frischauf I, Eshaghi S, Romanin C
J Biol Chem June 2009  

Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies.

Michalke K, Graviere ME, Huyghe C, Vincentelli R, Wagner R, Pattus F, Schroeder K, Oschmann J, Rudolph R, Cambillau C, Desmyter A
Anal Biochem (386) May 2009 147-55
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A novel, generic and effective method for the rapid purification of G protein-coupled receptors.

Magnin T, Fiez-Vandal C, Potier N, Coquard A, Leray I, Steffan T, Logez C, Alkhalfioui F, Pattus F, Wagner R
Protein Expr Purif (64) May 2009 1-7
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The multidrug resistance efflux complex, EmrAB from Escherichia coli forms a dimer in vitro.

Tanabe M, Szakonyi G, Brown KA, Henderson PJ, Nield J, Byrne B
Biochem Biophys Res Commun (380) May 2009 338-42
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High-level expression of rabies virus glycoprotein with the RNA-based Semliki Forest Virus expression vector.

Benmaamar R, Astray RM, Wagner R, Pereira CA
J Biotechnol (139) April 2009 283-90
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The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter.

Mulligan C, Geertsma ER, Severi E, Kelly DJ, Poolman B, Thomas GH
Proc Natl Acad Sci U S A (106) April 2009 1778-83
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The sodium-dependent D-glucose transport protein of Helicobacter pylori.

Psakis G, Saidijam M, Shibayama K, Polaczek J, Bettaney KE, Baldwin JM, Baldwin SA, Hope R, Essen LO, Essenberg RC, Henderson PJ
Mol Microbiol (71) Mar 2009 391-403
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High-throughput expression and detergent screening of integral membrane proteins.

Methods Mol Biol (498) Feb 2009 265-71

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield.

Bonander N, Darby RA, Grgic L, Bora N, Wen J, Brogna S, Poyner DR, O'Neill MA, Bill RM
Microb Cell Fact (8) Feb 2009 10
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Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.

Holmes WJ, Darby RA, Wilks MD, Smith R, Bill RM
Microb Cell Fact (8) Feb 2009 35

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2008

Crystallization of the hydantoin transporter Mhp1 from Microbacterium liquefaciens.

Shimamura T, Yajima S, Suzuki S, Rutherford NG, O'Reilly J, Henderson PJ, Iwata S
Acta Crystallogr Sect F Struct Biol Cryst Commun (64) 2008 1172-4

Exploring the activity of tobacco etch virus protease in detergent solutions.

Lundback AK, van den Berg S, Hebert H, Berglund H, Eshaghi S
Anal Biochem (382) 2008 69-71
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Mechanisms of YidC-mediated insertion and assembly of multimeric membrane protein complexes.

Kol S, Nouwen N, Driessen AJ
J Biol Chem (283) 2008 31269-73
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YidC is involved in the biogenesis of anaerobic respiratory complexes in the inner membrane of Escherichia coli.

Price CE, Driessen AJ
J Biol Chem (283) Dec 2008 26921-7
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Determination of the molecular mass and dimensions of membrane proteins by size exclusion chromatography.

Kunji ER, Harding M, Butler PJ, Akamine P
Methods (46) Dec 2008 62-72
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New and emerging methods in membrane protein research.

Methods (46) Dec 2008 47
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Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter.

Weyand S, Shimamura T, Yajima S, Suzuki S, Mirza O, Krusong K, Carpenter EP, Rutherford NG, Hadden JM, O'Reilly J, Ma P, Saidijam M, Patching SG, Hope RJ, Norbertczak HT, Roach PC, Iwata S, Henderson PJ, Cameron AD
Science (322) Dec 2008 709-13
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Expression, purification and activities of the entire family of intact membrane sensor kinases from Enterococcus faecalis.

Ma P, Yuille HM, Blessie V, Gohring N, Igloi Z, Nishiguchi K, Nakayama J, Henderson PJ, Phillips-Jones MK
Mol Membr Biol (25) Nov 2008 449-73
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Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR.

Patching SG, Psakis G, Baldwin SA, Baldwin J, Henderson PJ, Middleton DA
Mol Membr Biol (25) Nov 2008 474-84
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High-resolution x-ray structure of human aquaporin 5.

Horsefield R, Norden K, Fellert M, Backmark A, Tornroth-Horsefield S, Terwisscha van Scheltinga AC, Kvassman J, Kjellbom P, Johanson U, Neutze R
Proc Natl Acad Sci U S A (105) Nov 2008 13327-32
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Double-quantum 13C nuclear magnetic resonance of bathorhodopsin, the first photointermediate in mammalian vision.

Concistre M, Gansmuller A, McLean N, Johannessen OG, Marin Montesinos I, Bovee-Geurts PH, Verdegem P, Lugtenburg J, Brown RC, DeGrip WJ, Levitt MH
J Am Chem Soc (130) Oct 2008 10490-1
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Molecular mechanism of energy conservation in polysulfide respiration.

Jormakka M, Yokoyama K, Yano T, Tamakoshi M, Akimoto S, Shimamura T, Curmi P, Iwata S
Nat Struct Mol Biol (15) Sept 2008 730-7
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High-level expression, purification, and crystallization of recombinant rat leukotriene C(4) synthase from the yeast Pichia pastoris.

Wetterholm A, Molina DM, Nordlund P, Eshaghi S, Haeggstrom JZ
Protein Expr Purif (60) Sept 2008 1-6
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Expression and purification of the recombinant membrane protein YidC: A case study for increased stability and solubility.

Martinez Molina D, Lundback AK, Niegowski D, Eshaghi S
Protein Expr Purif July 2008  

Using EGFP fusions to monitor the functional expression of GPCRs in the Drosophila Schneider 2 cells.

Brillet K, Perret BG, Klein V, Pattus F, Wagner R
Cytotechnology (57) July 2008 101-9
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Engineering membrane protein overproduction in Escherichia coli.

Martinez Molina D, Cornvik T, Eshaghi S, Haeggstrom JZ, Nordlund P, Sabet MI
Protein Sci (17) June 2008 673-80
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The charge distribution in the cytoplasmic loop of subunit C of the F1F0 ATPase is a determinant for YidC targeting.

Kol S, Nouwen N, Driessen AJ
J Biol Chem (283) June 2008 9871-7
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Functional Expression, Targeting and Ca(2+) Signaling of a Mouse Melanopsin-eYFP Fusion Protein in a Retinal Pigment Epithelium Cell Line.

Giesbers ME, Shirzad-Wasei N, Bosman GJ, de Grip WJ
Photochem Photobiol June 2008  

Quality control of overexpressed membrane proteins.

Geertsma ER, Groeneveld M, Slotboom DJ, Poolman B
Proc Natl Acad Sci U S A (105) June 2008 5722-7
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Structural characterization of recombinant human CD81 produced in Pichia pastoris.

Jamshad M, Rajesh S, Stamataki Z, McKeating JA, Dafforn T, Overduin M, Bill RM
Protein Expr Purif (57) April 2008 206-16
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Solid-state NMR spectroscopy detects interactions between tryptophan residues of the E. coli sugar transporter GalP and the alpha-anomer of the D-glucose substrate.

Patching SG, Henderson PJ, Herbert RB, Middleton DA
J Am Chem Soc (130) Mar 2008 1236-44
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Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures.

Singh S, Gras A, Fiez-Vandal C, Ruprecht J, Rana R, Martinez M, Strange PG, Wagner R, Byrne B
Microb Cell Fact (7) Feb 2008 28
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Transcriptome analysis of a respiratory Saccharomyces cerevisiae strain suggests the expression of its phenotype is glucose insensitive and predominantly controlled by Hap4, Cat8 and Mig1.

Bonander N, Ferndahl C, Mostad P, Wilks MD, Chang C, Showe L, Gustafsson L, Larsson C, Bill RM
BMC Genomics (9) Feb 2008 365
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Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes.

Zweers JC, Barak I, Becher D, Driessen AJ, Hecker M, Kontinen VP, Saller MJ, Vavrova L, van Dijl JM
Microb Cell Fact (7) Feb 2008 10
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Protein translocation across the bacterial cytoplasmic membrane.

Driessen AJ, Nouwen N
Annu Rev Biochem (77) Feb 2008 643-67
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Membrane reconstitution of ABC transporters and assays of translocator function.

Geertsma ER, Nik Mahmood NA, Schuurman-Wolters GK, Poolman B
Nat Protoc (3) Feb 2008 256-66

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2007

Crystal structure of AcrB in complex with a single transmembrane subunit reveals another twist.

Tornroth-Horsefield S, Gourdon P, Horsefield R, Brive L, Yamamoto N, Mori H, Snijder A, Neutze R
Structure (15) 2007 1663-73
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Opsin stability and folding: modulation by phospholipid bicelles.

McKibbin C, Farmer NA, Jeans C, Reeves PJ, Khorana HG, Wallace BA, Edwards PC, Villa C, Booth PJ
J Mol Biol (374) 2007 1319-32
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Opsin stability and folding: the role of Cys185 and abnormal disulfide bond formation in the intradiscal domain.

McKibbin C, Toye AM, Reeves PJ, Khorana HG, Edwards PC, Villa C, Booth PJ
J Mol Biol (374) 2007 1309-18
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Expression screening of integral membrane proteins from Helicobacter pylori 26695.

Psakis G, Nitschkowski S, Holz C, Kress D, Maestre-Reyna M, Polaczek J, Illing G, Essen LO
Protein Sci (16) 2007 2667-76
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7,8-dihydro retinals outperform the native retinals in conferring photosensitivity to visual opsin.

DeGrip WJ, Bovee-Geurts PH, van der Hoef I, Lugtenburg J
J Am Chem Soc (129) Dec 2007 13265-9
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High-throughput cloning and expression in recalcitrant bacteria.

Nat Methods (4) Nov 2007 705-7
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Structural determination of wild-type lactose permease.

Guan L, Mirza O, Verner G, Iwata S, Kaback HR
Proc Natl Acad Sci U S A (104) Nov 2007 15294-8
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Introduction of a rod pigment aromatic cluster does not improve the structural stability of the human green cone pigment.

J Struct Biol (159) Oct 2007 222-7
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Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase.

Martinez Molina D, Wetterholm A, Kohl A, McCarthy AA, Niegowski D, Ohlson E, Hammarberg T, Eshaghi S, Haeggstrom JZ, Nordlund P
Nature (448) Oct 2007 613-6
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Yeast mitochondrial ADP/ATP carriers are monomeric in detergents as demonstrated by differential affinity purification.

Bamber L, Slotboom DJ, Kunji ER
J Mol Biol (371) Oct 2007 388-95
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A high-throughput method for membrane protein solubility screening: the ultracentrifugation dispersity sedimentation assay.

Gutmann DA, Mizohata E, Newstead S, Ferrandon S, Postis V, Xia X, Henderson PJ, van Veen HW, Byrne B
Protein Sci (16) Sept 2007 1422-8
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The yeast mitochondrial ADP/ATP carrier functions as a monomer in mitochondrial membranes.

Bamber L, Harding M, Monne M, Slotboom DJ, Kunji ER
Proc Natl Acad Sci U S A (104) Aug 2007 10830-4
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Differential structural properties of GLP-1 and exendin-4 determine their relative affinity for the GLP-1 receptor N-terminal extracellular domain.

Runge S, Schimmer S, Oschmann J, Schiodt CB, Knudsen SM, Jeppesen CB, Madsen K, Lau J, Thogersen H, Rudolph R
Biochemistry (46) July 2007 5830-40
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Metabolism of glutamine and glutathione via gamma-glutamyltranspeptidase and glutamate transport in Helicobacter pylori: possible significance in the pathophysiology of the organism.

Shibayama K, Wachino J, Arakawa Y, Saidijam M, Rutherford NG, Henderson PJ
Mol Microbiol (64) June 2007 396-406
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Flavin binding to the high affinity riboflavin transporter RibU.

Duurkens RH, Tol MB, Geertsma ER, Permentier HP, Slotboom DJ
J Biol Chem (282) June 2007 10380-6
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Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides.

Roszak AW, Gardiner AT, Isaacs NW, Cogdell RJ
Biochemistry (46) May 2007 2909-16
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pH dependence of copper geometry, reduction potential, and nitrite affinity in nitrite reductase.

Jacobson F, Pistorius A, Farkas D, De Grip W, Hansson O, Sjolin L, Neutze R
J Biol Chem (282) May 2007 6347-55
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Solid-state NMR evidence for a protonation switch in the binding pocket of the H1 receptor upon binding of the agonist histamine.

Ratnala VR, Kiihne SR, Buda F, Leurs R, de Groot HJ, DeGrip WJ
J Am Chem Soc (129) Mar 2007 867-72
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Light stimulates growth of proteorhodopsin-containing marine Flavobacteria.

Gomez-Consarnau L, Gonzalez JM, Coll-Llado M, Gourdon P, Pascher T, Neutze R, Pedros-Alio C, Pinhassi J
Nature (445) Mar 2007 210-3
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Modulation of proton-induced current fluctuations in the human nicotinic acetylcholine receptor channel.

Danelon C, Grandl J, Hovius R, Vogel H
Biochim Biophys Acta (1768) Mar 2007 76-89
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2006

Fabrication and functionalization of nanochannels by electron-beam-induced silicon oxide deposition.

Danelon C, Santschi C, Brugger J, Vogel H
Langmuir (22) 2006 10711-5
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Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris.

Zeder-Lutz G, Cherouati N, Reinhart C, Pattus F, Wagner R
Protein Expr Purif (50) 2006 118-27
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Lipidic sponge phase crystallization of membrane proteins.

Wadsten P, Wohri AB, Snijder A, Katona G, Gardiner AT, Cogdell RJ, Neutze R, Engstrom S
J Mol Biol (364) 2006 44-53
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Functional asymmetry of transmembrane segments in nicotinic acetylcholine receptors.

Grandl J, Danelon C, Hovius R, Vogel H
Eur Biophys J (35) Dec 2006 685-93
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Ion specificity and ionic strength dependence of the osmoregulatory ABC transporter OpuA.

Mahmood NA, Biemans-Oldehinkel E, Patzlaff JS, Schuurman-Wolters GK, Poolman B
J Biol Chem (281) Dec 2006 29830-9
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YidC-mediated membrane insertion of assembly mutants of subunit c of the F1F0 ATPase.

Kol S, Turrell BR, de Keyzer J, van der Laan M, Nouwen N, Driessen AJ
J Biol Chem (281) Dec 2006 29762-8
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Yeast mitochondrial ADP/ATP carriers are monomeric in detergents.

Bamber L, Harding M, Butler PJ, Kunji ER
Proc Natl Acad Sci U S A (103) Dec 2006 16224-9
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Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae.

Pettersson N, Hagstrom J, Bill RM, Hohmann S
Curr Genet (50) Dec 2006 247-55
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Comparative analysis and 'expression space' coverage of the production of prokaryotic membrane proteins for structural genomics.

Surade S, Klein M, Stolt-Bergner PC, Muenke C, Roy A, Michel H
Protein Sci (15) Nov 2006 2178-89
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Identification of two interaction sites in SecY that are important for the functional interaction with SecA.

van der Sluis EO, Nouwen N, Koch J, de Keyzer J, van der Does C, Tampe R, Driessen AJ
J Mol Biol (361) Nov 2006 839-49
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A simple strategy towards membrane protein purification and crystallization.

Niegowski D, Hedren M, Nordlund P, Eshaghi S
Int J Biol Macromol (39) Oct 2006 83-7
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A sensor for intracellular ionic strength.

Biemans-Oldehinkel E, Mahmood NA, Poolman B
Proc Natl Acad Sci U S A (103) Sept 2006 10624-9
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Crystal structure of a divalent metal ion transporter CorA at 2.9 angstrom resolution.

Eshaghi S, Niegowski D, Kohl A, Martinez Molina D, Lesley SA, Nordlund P
Science (313) Sept 2006 354-7
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Isolation of Escherichia coli inner membranes by metal affinity two-phase partitioning.

Everberg H, Clough J, Henderson P, Jergil B, Tjerneld F, Ramirez IB
J Chromatogr A (1118) Aug 2006 244-52
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Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems.

Lundstrom K, Wagner R, Reinhart C, Desmyter A, Cherouati N, Magnin T, Zeder-Lutz G, Courtot M, Prual C, Andre N, Hassaine G, Michel H, Cambillau C, Pattus F
J Struct Funct Genomics (7) Aug 2006 77-91
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Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen.

Andre N, Cherouati N, Prual C, Steffan T, Zeder-Lutz G, Magnin T, Pattus F, Michel H, Wagner R, Reinhart C
Protein Sci (15) July 2006 1115-26
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The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism.

Burgess CM, Slotboom DJ, Geertsma ER, Duurkens RH, Poolman B, van Sinderen D
J Bacteriol (188) June 2006 2752-60
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Optimization of membrane protein overexpression and purification using GFP fusions.

Drew D, Lerch M, Kunji E, Slotboom DJ, de Gier JW
Nat Methods (3) June 2006 303-13
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Covalent labeling of cell-surface proteins for in-vivo FRET studies.

Meyer BH, Martinez KL, Segura JM, Pascoal P, Hovius R, George N, Johnsson K, Vogel H
FEBS Lett (580) May 2006 1654-8
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Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy.

Lai WC, McLean N, Gansmuller A, Verhoeven MA, Antonioli GC, Carravetta M, Duma L, Bovee-Geurts PH, Johannessen OG, de Groot HJ, Lugtenburg J, Emsley L, Brown SP, Brown RC, DeGrip WJ, Levitt MH
J Am Chem Soc (128) May 2006 3878-9
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Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY.

Mirza O, Guan L, Verner G, Iwata S, Kaback HR
EMBO J (25) May 2006 1177-83
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Semliki Forest virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells.

Hassaine G, Wagner R, Kempf J, Cherouati N, Hassaine N, Prual C, Andre N, Reinhart C, Pattus F, Lundstrom K
Protein Expr Purif (45) April 2006 343-51
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Mitochondrial carriers in the cytoplasmic state have a common substrate binding site.

Robinson AJ, Kunji ER
Proc Natl Acad Sci U S A (103) April 2006 2617-22
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Structural mechanism of plant aquaporin gating.

Tornroth-Horsefield S, Wang Y, Hedfalk K, Johanson U, Karlsson M, Tajkhorshid E, Neutze R, Kjellbom P
Nature (439) April 2006 688-94
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Experiment and theory for heterogeneous nucleation of protein crystals in a porous medium.

Chayen NE, Saridakis E, Sear RP
Proc Natl Acad Sci U S A (103) Mar 2006 597-601
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Cell membranes suspended across nanoaperture arrays.

Danelon C, Perez JB, Santschi C, Brugger J, Vogel H
Langmuir (22) Mar 2006 22-5
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2005

Functional expression of eukaryotic membrane proteins in Lactococcus lactis.

Monne M, Chan KW, Slotboom DJ, Kunji ER
Protein Sci (14) 2005 3048-56
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In vitro functional characterization of BtuCD-F, the Escherichia coli ABC transporter for vitamin B12 uptake.

Borths EL, Poolman B, Hvorup RN, Locher KP, Rees DC
Biochemistry (44) 2005 16301-9
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The activity of the lactose transporter from Streptococcus thermophilus is increased by phosphorylated IIA and the action of beta-galactosidase.

Geertsma ER, Duurkens RH, Poolman B
Biochemistry (44) 2005 15889-97
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Covalently dimerized SecA is functional in protein translocation.

de Keyzer J, van der Sluis EO, Spelbrink RE, Nijstad N, de Kruijff B, Nouwen N, van der Does C, Driessen AJ
J Biol Chem (280) Dec 2005 35255-60
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Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose concentrations.

Henricsson C, de Jesus Ferreira MC, Hedfalk K, Elbing K, Larsson C, Bill RM, Norbeck J, Hohmann S, Gustafsson L
Appl Environ Microbiol (71) Dec 2005 6185-92
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Electrochemical structure of the crowded cytoplasm.

Spitzer JJ, Poolman B
Trends Biochem Sci (30) Dec 2005 536-41
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Functional interactions between the subunits of the lactose transporter from Streptococcus thermophilus.

Geertsma ER, Duurkens RH, Poolman B
J Mol Biol (350) Sept 2005 102-11
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Design of improved membrane protein production experiments: quantitation of the host response.

Bonander N, Hedfalk K, Larsson C, Mostad P, Chang C, Gustafsson L, Bill RM
Protein Sci (14) Sept 2005 1729-40
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Normal mode analysis suggests a quaternary twist model for the nicotinic receptor gating mechanism.

Taly A, Delarue M, Grutter T, Nilges M, Le Novere N, Corringer PJ, Changeux JP
Biophys J (88) Aug 2005 3954-65
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Substrate specificity and ionic regulation of GlnPQ from Lactococcus lactis. An ATP-binding cassette transporter with four extracytoplasmic substrate-binding domains.

Schuurman-Wolters GK, Poolman B
J Biol Chem (280) Aug 2005 23785-90
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Structure of the rotor of the V-Type Na+-ATPase from Enterococcus hirae.

Murata T, Yamato I, Kakinuma Y, Leslie AG, Walker JE
Science (308) June 2005 654-9
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An efficient strategy for high-throughput expression screening of recombinant integral membrane proteins.

Eshaghi S, Hedren M, Nasser MI, Hammarberg T, Thornell A, Nordlund P
Protein Sci (14) May 2005 676-83
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The crystal structure of the pyoverdine outer membrane receptor FpvA from Pseudomonas aeruginosa at 3.6 angstroms resolution.

Cobessi D, Celia H, Folschweiller N, Schalk IJ, Abdallah MA, Pattus F
J Mol Biol (347) May 2005 121-34
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The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane.

Vervoort EB, Bultema JB, Schuurman-Wolters GK, Geertsma ER, Broos J, Poolman B
J Mol Biol (346) April 2005 733-43
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Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles.

Doeven MK, Folgering JH, Krasnikov V, Geertsma ER, van den Bogaart G, Poolman B
Biophys J (88) April 2005 1134-42
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Conditional osmotic stress in yeast: a system to study transport through aquaglyceroporins and osmostress signaling.

Karlgren S, Pettersson N, Nordlander B, Mathai JC, Brodsky JL, Zeidel ML, Bill RM, Hohmann S
J Biol Chem (280) April 2005 7186-93
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2004

Switching the mode of metabolism in the yeast Saccharomyces cerevisiae.

Otterstedt K, Larsson C, Bill RM, Stahlberg A, Boles E, Hohmann S, Gustafsson L
EMBO Rep (5) July 2004 532-7
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F1F0 ATP synthase subunit c is a substrate of the novel YidC pathway for membrane protein biogenesis.

van der Laan M, Bechtluft P, Kol S, Nouwen N, Driessen AJ
J Cell Biol (165) June 2004 213-22
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2003

Expression of EGFP-amino-tagged human mu opioid receptor in Drosophila Schneider 2 cells: a potential expression system for large-scale production of G-protein coupled receptors.

Perret BG, Wagner R, Lecat S, Brillet K, Rabut G, Bucher B, Pattus F
Protein Expr Purif (31) Nov 2003 123-32
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