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MELTING
Sequence
(one per line)
Complementary sequence
(one per line)
Sliding window
(enter size of window if needed)
Hybridisation type
DNA/DNA
DNA/RNA
RNA/RNA
Nearest neighbour parameters set
Allawi et al 1997
SantaLucia et al 2004
SantaLucia et al 1996
Breslauer et al 1986
Sugimoto et al 1995
Xia et al 1998
Freier et al 1986
Salt concentration (0.0 <= x < 10.0 M)
Potassium concentration (x>= 0.0 M)
Magnesium concentration (x>= 0.0 M)
Tris buffer concentration (x>= 0.0 M)
Nucleic acid concentration in exess (0.0 < x < 0.1 M)
Nucleic acid correction factor
Salt correction
Wetmur 1991
SantaLucia et al 1996
SantaLucia et al 1998
Force approximate temperature computation
Parameters for dangling ends: dnadnade.nn
Nearest neighbour parameters for mismatches: dnadnamm.nn
Nearest neighbour parameters for inosines mismatches
in DNA: Santalucia et al 2005
in RNA: Brent M. Znosko et al 2007
