ESTDAB Cell Profile for ESTDAB-202 (Ma-Mel-59a)


Cell Source

Cell Type: MelanomaTissue of Origin: Unknown
Ethnic Origin: UnknownGender: Unknown
Medium Used: RPMI 1640, 10% FBSMorphology: Unknown
Mycoplasma: -  
Comments: same DNA-profile as Ma-Mel-59b (ESTDAB-216), Ma-Mel-59c (ESTDAB-217) and ESTDAB-201

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Cell Image (200x Magnification)

Primary Search Determinants

High Resolution HLA Typing

High Resolution HLA Typing

High Resolution Typing of HLA and Related Genes (Help on HLA High Resolution Typing)
HLA-AND
HLA-BND
HLA-CND
HLA-DRB1ND
HLA-DRB3ND
HLA-DRB4ND
HLA-DRB5ND
HLA-DQA1ND
HLA-DQB1ND
HLA-DPA1ND
HLA-DPB1ND

HLA and Related Genes, Surface Expression

HLA and Related Genes, Surface Expression

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
HLA-AND0ND0
HLA-A,B,CND0ND0
HLA-BND0ND0
HLA-Bw4ND0ND0
HLA-Bw6ND0ND0
HLA-CND0ND0
HLA-DPND0ND0
HLA-DQND0ND0
HLA-DRND0ND0
HLA-END0ND0
HLA-GND0ND0
MICAND0ND0
MICA/BND0ND0
MICBND0ND0
B2mND0ND0

Tumour Assosciated Antigens

Tumour Assosciated Antigens

Tumour Assosciated Antigens (Help on Tumour Assosciated Antigens)
Marker Score Marker Score
BAGENDMAGE 6 ND 
BUC1NDMart 1 ND 
GAGENDMMP2 ND 
gp100NDMTA-1 ND 
MAGE 1NDMUC-1 ND 
MAGE 10NDNA17-A ND 
MAGE 12NDNY ESO ND 
MAGE 2NDSURVIVIN ND 
MAGE 3NDT128 ND 
MAGE 4NDT17 ND 
MAGE 6NDT21 ND 
Mart 1NDTACC1D ND 
MMP2NDTRAG 3 ND 
MTA-1NDTRP 1 ND 
Chromium Release Assay (Help on Chromium Release Assay)
Marker Specificity Ratio Lysis (%)
gp100 (154-162)ND ND ND 
gp100 (209-217)ND ND ND 
Mart1 (27-25)ND ND ND 
Immunohistochemistry (Help on Immunohistochemistry)
Marker Score  
gp100ND  

Cytokine Secretions

Cytokine Secretions

Cytokine Secretion Data (Help on Cytokine Secretions)
Marker Substrate - Plastic Marker Substrate - Matrigel
Interferon Gamma  Interferon Gamma  
Interleukin 10  Interleukin 10  
Interleukin 6  Interleukin 6  
Interleukin 8  Interleukin 8  
TGF Beta  TGF Beta  
TNF Alpha  TNF Alpha  

Secondary Search Determinants

Antigen Processing

Antigen Processing

Western Blot Data (Help on Western Blot Data)
Marker Initial After 72hrs IFN-Gamma Induced
Heavy ChainNDND
LMP2NDND
LMP7NDND
MECL1NDND
TAP1NDND
TAP2NDND

Apoptosis

Apoptosis

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
B7H1ND0ND0
CD95ND0ND0
CD95LND0ND0
PD-1ND0ND0
TRAIL-R1ND0ND0
TRAIL-R2ND0ND0
TRAIL-R3ND0ND0
TRAIL-R4ND0ND0
RNA Protection Assay Data (Help on RNA Protection Assay)
Marker Score Marker Score
caspase-8NDRIPND
cIAP1NDTNFRp55ND
cIAP2NDTRADDND
DR3 TRAMP APO3NDTRAF1ND
FADDNDTRAF2ND
FAFNDTRAF3ND
FAPNDTRAF4ND
FasNDTRAILND
FasLNDTRPM2ND
NAIPNDXIAPND
Western Blot Data (Help on Western Blot Data)
Marker Score Marker Score

Cytokine Polymorphisms

Cytokine Polymorphisms

Cytokine Polymorphism Data (Help on Cytokine Polymorphisms)
Marker Position Polymorphism Polymorphism
Interferon Gamma-333  
Interferon Gamma874  
Interleukin 10-1082  
Interleukin 10-819  
Interleukin 10-592  
Interleukin 10571  
Interleukin 6-174  
TGF Beta-988  
TGF Beta-800  
TGF Beta-509  
TGF Betacodon 10  
TGF Betacodon 25  
TGF Beta652  
TGF Beta653  
TGF Beta713  
TGF Beta788  
TGF Beta869  
TGF Beta915  
TNF Alpha-1031  
TNF Alpha-863  
TNF Alpha-857  
TNF Alpha-851  
TNF Alpha-574  
TNF Alpha-376  
TNF Alpha-308  
TNF Alpha-238  
TNF Alpha488  

DNA Profile

DNA Profile

DNA Profile (Help on DNA Profiles)
Marker Score Marker Score
AmelogeninXD7S820 8, 12 
CSF1PO10FGA 20, 22 
D13S31712, 13 THO1 
D3S135817TPOX 9, 11 
D5S81812, 13 vWA 17 

Extra Cellular Matrix

Extra Cellular Matrix

Cell Adhesion (Help on Cell Adhesion)
Marker Score Marker Score
CollagenNDLaminin ND 
FibronectinND  
Glycosyltransferases (Help on Glycosyltransferases)
Marker Score Marker Score
FUT1NDSIAT3 ND 
FUT4NDSIAT4C ND 
MGAT5ND  
Lectins (Help on Lectins)
Mol. Weight (KDa) DSA GNA LTA NAA PHA-L SBA SNA
<30 ND ND ND ND ND ND ND
31-40 ND ND ND ND ND ND ND
41-50 ND ND ND ND ND ND ND
51-60 ND ND ND ND ND ND ND
61-70 ND ND ND ND ND ND ND
71-80 ND ND ND ND ND ND ND
81-90 ND ND ND ND ND ND ND
91-100 ND ND ND ND ND ND ND
101-110 ND ND ND ND ND ND ND
111-120 ND ND ND ND ND ND ND
121-130 ND ND ND ND ND ND ND
131-140 ND ND ND ND ND ND ND
141-150 ND ND ND ND ND ND ND
151-160 ND ND ND ND ND ND ND
161-170 ND ND ND ND ND ND ND
171-180 ND ND ND ND ND ND ND
181-190 ND ND ND ND ND ND ND
191-200 ND ND ND ND ND ND ND
>200 ND ND ND ND ND ND ND

Surface Markers

Surface Markers

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
CD112ND0ND0
CD11aND0ND0
CD11bND0ND0
CD155ND0ND0
CD40ND0ND0
CD44ND0ND0
CD48ND0ND0
CD49dND0ND0
CD54ND0ND0
CD56ND0ND0
CD57ND0ND0
CD58ND0ND0
CD80ND0ND0
CD86ND0ND0
CD95ND0ND0
CD95LND0ND0
ULBP1ND0ND0
ULBP2ND0ND0
ULBP3ND0ND0
gp100ND0ND0
Immunohistochemistry (Help on Immunohistochemistry)
Marker Score Marker Score
P-Cadherin ND 
K-CadherinNDVE-Cadherin ND 
N-CadherinND  
gp100NDSurvivin ND 

(Epi)Genetic Analysis

(Epi)Genetic Analysis

(Epi)Genetic Analysis (Help on (Epi)Genetic Analysis)
Marker Mutation Deletion Methylation
Presence Description Presence Description Presence
APCNDNDN/AN/AND
BRAFNDNDN/AN/AN/A
CDK4NDNDN/AN/AN/A
CTNNB1NDNDN/AN/AN/A
NRASNDNDN/AN/AN/A
PTENNDNDNDNDN/A
RASSFIAN/AN/AN/AN/AND
RB1NDNDNDNDN/A
TP53NDNDN/AN/AN/A
p16NDNDNDNDND
p27N/AN/AN/AN/AND