ESTDAB Cell Profile for ESTDAB-076 (WM-266-4)


Cell Source

Cell Type: MelanomaTissue of Origin: Skin
Ethnic Origin: UnknownGender: Female
Medium Used: RPMI 1640, 10% FBSMorphology: Epithelial
Mycoplasma: -  
Comments: Derived from metastastic lesion; same patient as ESTDAB-066

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Cell Image (200x Magnification)

Primary Search Determinants

High Resolution HLA Typing

High Resolution HLA Typing

High Resolution Typing of HLA and Related Genes (Help on HLA High Resolution Typing)
HLA-A2902, 0201/09/43N
HLA-B440301, 1302
HLA-C0602, 1601/0801
HLA-DRB10701
HLA-DRB3ND
HLA-DRB4ND
HLA-DRB5ND
HLA-DQA1ND
HLA-DQB10201/02
HLA-DPA1ND
HLA-DPB1020102

HLA and Related Genes, Surface Expression

HLA and Related Genes, Surface Expression

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
HLA-A++176ND0
HLA-A,B,C+6.12ND0
HLA-B++122ND0
HLA-Bw4+49ND0
HLA-Bw6-4ND0
HLA-CND0ND0
HLA-DP-4ND0
HLA-DQ-1ND0
HLA-DR+2.13ND0
HLA-END0ND0
HLA-GND0ND0
MICAND0ND0
MICA/BND0ND0
MICBND0ND0
B2mND0ND0

Tumour Assosciated Antigens

Tumour Assosciated Antigens

Tumour Assosciated Antigens (Help on Tumour Assosciated Antigens)
Marker Score Marker Score
BAGE-MAGE 6 ++ 
BUC1NDMart 1 +++ 
GAGE++MMP2 ND 
gp100+++MTA-1 ND 
MAGE 1++MUC-1 
MAGE 10+++NA17-A ND 
MAGE 12+++NY ESO 
MAGE 2+SURVIVIN ++ 
MAGE 3+++T128 ND 
MAGE 4+T17 ND 
MAGE 6++T21 ND 
Mart 1+++TACC1D ND 
MMP2NDTRAG 3 ++ 
MTA-1NDTRP 1 
Chromium Release Assay (Help on Chromium Release Assay)
Marker Specificity Ratio Lysis (%)
gp100 (154-162)   
gp100 (209-217)   
Mart1 (27-25)HLA-02.01 5:1 12 
Immunohistochemistry (Help on Immunohistochemistry)
Marker Score  
gp100 

Cytokine Secretions

Cytokine Secretions

Cytokine Secretion Data (Help on Cytokine Secretions)
Marker Substrate - Plastic Marker Substrate - Matrigel
Interferon Gamma Interferon Gamma 
Interleukin 10 866.3 Interleukin 10 622.4 
Interleukin 6  Interleukin 6  
Interleukin 8 2000 Interleukin 8 2000 
TGF Beta  TGF Beta 767.5 
TNF Alpha TNF Alpha 

Secondary Search Determinants

Antigen Processing

Antigen Processing

Western Blot Data (Help on Western Blot Data)
Marker Initial After 72hrs IFN-Gamma Induced
Heavy ChainNDND
LMP2NDND
LMP7NDND
MECL1NDND
TAP1NDND
TAP2NDND

Apoptosis

Apoptosis

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
B7H1ND0ND0
CD95+9.21ND0
CD95L-1.03ND0
PD-1ND0ND0
TRAIL-R1-1.12ND0
TRAIL-R2+2.68ND0
TRAIL-R3-1.25ND0
TRAIL-R4-1.09ND0
RNA Protection Assay Data (Help on RNA Protection Assay)
Marker Score Marker Score
caspase-8NDRIPND
cIAP1NDTNFRp55ND
cIAP2NDTRADDND
DR3 TRAMP APO3NDTRAF1ND
FADDNDTRAF2ND
FAFNDTRAF3ND
FAPNDTRAF4ND
FasNDTRAILND
FasLNDTRPM2ND
NAIPNDXIAPND
Western Blot Data (Help on Western Blot Data)
Marker Score Marker Score
APAF1NDBcl xLND
BAXNDBcl xSND
Bcl 2NDBidND
Bcl wNDFLIPND

Cytokine Polymorphisms

Cytokine Polymorphisms

Cytokine Polymorphism Data (Help on Cytokine Polymorphisms)
Marker Position Polymorphism Polymorphism
Interferon Gamma-333
Interferon Gamma874
Interleukin 10-1082
Interleukin 10-819
Interleukin 10-592
Interleukin 10571
Interleukin 6-174
TGF Beta-988
TGF Beta-800
TGF Beta-509  
TGF Betacodon 10
TGF Betacodon 25
TGF Beta652  
TGF Beta653  
TGF Beta713  
TGF Beta788  
TGF Beta869
TGF Beta915
TNF Alpha-1031
TNF Alpha-863
TNF Alpha-857
TNF Alpha-851
TNF Alpha-574
TNF Alpha-376
TNF Alpha-308
TNF Alpha-238
TNF Alpha488

DNA Profile

DNA Profile

DNA Profile (Help on DNA Profiles)
Marker Score Marker Score
AmelogeninXD7S820 
CSF1PO12FGA 21, 23 
D13S31712, 13 THO1 7, 9 
D3S135815, 18 TPOX 8, 11 
D5S81813vWA 15, 17 

Extra Cellular Matrix

Extra Cellular Matrix

Cell Adhesion (Help on Cell Adhesion)
Marker Score Marker Score
Collagen+Laminin 
Fibronectin+  
Glycosyltransferases (Help on Glycosyltransferases)
Marker Score Marker Score
FUT1+SIAT3 
FUT4+SIAT4C 
MGAT5+  
Lectins (Help on Lectins)
Mol. Weight (KDa) DSA GNA LTA NAA PHA-L SBA SNA
<30 1 1 - - - ND -
31-40 - 1 1 - - ND -
41-50 2 - 1 - - ND 1
51-60 3 2 2 - - ND -
61-70 - - 1 - - ND 1
71-80 - - 1 - - ND 2
81-90 - 1 1 - - ND 1
91-100 1 1 1 2 1 ND 1
101-110 - 1 1 1 - ND 1
111-120 - 1 - - - ND 1
121-130 1 - - 1 - ND -
131-140 - 1 1 - - ND -
141-150 - - - - 1 ND 1
151-160 - - - - - ND -
161-170 - - - - - ND 1
171-180 - - - - - ND -
181-190 1 1 1 1 - ND -
191-200 - - 1 - 1 ND -
>200 3 - 2 2 1 ND 3

Surface Markers

Surface Markers

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
CD112ND0ND0
CD11aND0ND0
CD11bND0ND0
CD155ND0ND0
CD40-1.07ND0
CD44++15.44ND0
CD48ND0ND0
CD49dND0ND0
CD54++25.47ND0
CD56ND0ND0
CD57ND0ND0
CD58+8.87ND0
CD80-1.13ND0
CD86-1.05ND0
CD95+9.21ND0
CD95L-1.03ND0
ULBP1ND0ND0
ULBP2ND0ND0
ULBP3ND0ND0
gp100ND0ND0
Immunohistochemistry (Help on Immunohistochemistry)
Marker Score Marker Score
P-Cadherin 
K-CadherinNDVE-Cadherin 
N-Cadherin+  
gp100+Survivin 

(Epi)Genetic Analysis

(Epi)Genetic Analysis

(Epi)Genetic Analysis (Help on (Epi)Genetic Analysis)
Marker Mutation Deletion Methylation
Presence Description Presence Description Presence
APCNDNDN/AN/AND
BRAFNDNDN/AN/AN/A
CDK4NDNDN/AN/AN/A
CTNNB1NDNDN/AN/AN/A
NRASNDNDN/AN/AN/A
PTENNDNDNDNDN/A
RASSFIAN/AN/AN/AN/AND
RB1NDNDNDNDN/A
TP53NDNDN/AN/AN/A
p16NDNDNDNDND
p27N/AN/AN/AN/AND